Abstract：
:Transgenic tobacco plants expressing the hyperthermostable beta-glucosidase BglB of Thermotoga maritima were generated with the goal of cost-effective production of the enzyme for the application in bioconversion of lignocellulosic biomass. The enzyme was targeted to the cytosol and chloroplasts, where it accumulated to level of 4.5% and 5.8% of total soluble protein, respectively. The optimal temperature and pH of the plant-expressed BglB was 80 degrees C and 4.5, respectively. BglB activity was preserved in leaves after lyophilization, but decreased by over 70% with drying at room temperature. When BglB was synergistically supplied in a 1% (w/v) rice straw with Cel5A for efficient cellulase conversion, a 37% increase in glucose was observed. This report demonstrates the potential of utilizing transgenic tobacco for mass production of BglB.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Jung S,Kim S,Bae H,Lim HS,Bae HJdoi
10.1016/j.biortech.2010.03.140subject
Has Abstractpub_date
2010-09-01 00:00:00pages
7155-61issue
18eissn
0960-8524issn
1873-2976pii
S0960-8524(10)00659-0journal_volume
101pub_type
杂志文章abstract：:The effect of a "controlled temperature" approach was investigated in the microwave-enhanced simultaneous extraction and transesterification of dry algae. Experimental runs were designed using a response surface methodology (RSM). The process parameters such as dry algae to methanol ratio, reaction time, and catalyst ...
journal_title：Bioresource technology
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/j.biortech.2007.09.092
更新日期：2008-09-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
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更新日期：2020-08-01 00:00:00
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更新日期：2018-02-01 00:00:00
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journal_title：Bioresource technology
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doi：10.1016/s0960-8524(03)00193-7
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journal_title：Bioresource technology
pub_type： 杂志文章,评审
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更新日期：2016-09-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.02.052
更新日期：2013-04-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.10.086
更新日期：2012-01-01 00:00:00
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journal_title：Bioresource technology
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doi：10.1016/j.biortech.2011.06.027
更新日期：2011-09-01 00:00:00
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journal_title：Bioresource technology
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更新日期：2016-11-01 00:00:00
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更新日期：2017-02-01 00:00:00
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更新日期：2009-06-01 00:00:00
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journal_title：Bioresource technology
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更新日期：2011-12-01 00:00:00
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更新日期：2017-01-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
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更新日期：2013-01-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
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更新日期：2021-01-01 00:00:00
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journal_title：Bioresource technology
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doi：10.1016/j.biortech.2020.124541
更新日期：2020-12-14 00:00:00
