Abstract：
:A Xylose reductase (XR) from the halotolerant yeast, Debaryomyces nepalensis NCYC 3413 was purified to apparent homogeneity. The enzyme has a molecular mass of 74 kDa with monomeric subunit of 36.4 kDa (MALDI-TOF/MS) and pI of 6.0. The enzyme exhibited its maximum activity at pH 7.0 and 45 °C (21.2U/mg). In situ gel digestion and peptide mass fingerprinting analysis showed 12-22% sequence homology with XR from other yeasts. Inhibition of the enzyme by DEPC (diethylpyrocarbonate) confirmed the presence of histidine residue in its active site. The enzyme exhibited high preference for pentoses over hexoses with greater catalytic efficiency for arabinose than xylose. The enzyme also showed absolute specificity with NADPH over NADH. The enzyme retained 90% activity with 100 mM of NaCl or KCl and 40% activity with 1 M KCl which suggest that the enzyme is moderately halotolerant and can be utilized for commercial production of xylitol under conditions where salts are present.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Kumar S,Gummadi SNdoi
10.1016/j.biortech.2011.07.030subject
Has Abstractpub_date
2011-10-01 00:00:00pages
9710-7issue
20eissn
0960-8524issn
1873-2976pii
S0960-8524(11)00956-4journal_volume
102pub_type
杂志文章abstract：:This study examine ways to make biorefinery lignin accessible for anaerobic digestion. The raw material was the residue after removing carbohydrates by Wet Explosion pretreatment at 190 °C and 7.5% O2 followed by enzymatic hydrolysis. The residual solid was mainly composed of lignin and was the raw material for a seco...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.122537
更新日期：2020-02-01 00:00:00
abstract：:This work presents a Life Cycle Assessment (LCA) of bioethanol (EtOH) from perennial Arundo donax L. feedstock. A "cradle-to-wheel" approach was applied considering primary data for the cultivation of dedicated crops on hilly marginal lands and innovative "second generation technologies" for feedstock conversion into ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.08.022
更新日期：2016-11-01 00:00:00
abstract：:Eleven test runs (including two replicates) were carried out to explore the interaction of shear stress and chlorine concentration on the growth of heterotrophic microorganisms. Experimental results revealed that influent chlorine concentration and shear stress had no interaction on biofilm formation. Biofilm bacteria...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2005.08.003
更新日期：2006-10-01 00:00:00
abstract：:Baffles are a key component of an airlift membrane bioreactor (MBR), which could enhance membrane surface shear for fouling control. In order to obtain an optimal hydraulic condition of the reactor, the effects of baffle location and size were systematically explored in this study. Computational fluid dynamics (CFD) w...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.10.133
更新日期：2015-01-01 00:00:00
abstract：:Sequential batch thermophilic (55°C) solid-state anaerobic digestion (SS-AD) of yard trimmings was evaluated at a total solids (TS) content of 22% via recirculating digestate as the inoculum. The substrate-to-inoculum (S/I) ratio of 1 (TS basis) was favored over 2 and 3 due to significantly higher methane yield and vo...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.03.136
更新日期：2017-07-01 00:00:00
abstract：:A model describing ibuprofen and ketoprofen biodegradation by activated sludge from three different wastewater treatment plants (WWTP) was developed in this study. This model successfully described the biodegradation profiles observed at two different initial concentrations of each compound, where a lag-phase was obse...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.01.035
更新日期：2013-04-01 00:00:00
abstract：:As antibiotic and heavy metals are over used in the livestock industry, animal manure is a reservoir of antibiotic resistance genes (ARGs). Anaerobic digestion has been reported to have the potential to reduce ARGs. However, few studies investigated whether reduction of ARGs would be affected by different external pre...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.03.134
更新日期：2017-08-01 00:00:00
abstract：:An A. nidulans strain with a pyridoxine marker was used for continuous production of aryl alcohol oxidase (AAO) in a trickle bed reactor (TBR). Modified medium with reduced zinc, no copper, and 5 g/L ascorbic acid that reduced melanin production and increased AAO productivity under growth limited conditions was used. ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.01.098
更新日期：2018-05-01 00:00:00
abstract：:A FeCl3 pretreatment methodology was developed to convert raw sugarcane bagasse to highly digestible pretreated solid and selectively extract up to ∼100% of the hemicellulose from lignocellulosic biomass. FeCl3 pretreated solids yielded a quite high fermentable sugar yield compared to the native material. In addition,...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.01.013
更新日期：2017-04-01 00:00:00
abstract：:Optimum culture conditions which ease the synthesis of a novel exopolysaccharide (EPS) from a potent marine strain Streptococcus phocae was proposed in this study. The strain grows well at 35 °C, pH 7.0 and NaCl (2%) with lactose and yeast extract as best carbon and nitrogen sources. The maximum yield of EPS (11.75 an...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.12.118
更新日期：2011-04-01 00:00:00
abstract：:A process of intermittently cyclic activated sludge-membrane bioreactor (ICAS-MBR) was developed to enhance the performance of biological phosphorus removal (EBPR), which was constructed under sequencing anoxic/anaerobic/aerobic condition. The performance between ICAS-MBR and conventional anoxic/aerobic-membrane biore...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.02.054
更新日期：2009-09-01 00:00:00
abstract：:Rapid formation of denitrifying sulfide removal granules is of practical interest to start up an expanded granular sludge bed reactor for wastewater treatment. This study demonstrates that methanogenic granules can be easily acclimated into DSR granules in one day, removing all 1.30 kg m(-3) d(-1) sulfide and converti...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.01.049
更新日期：2011-04-01 00:00:00
abstract：:The aim of this study was to assess the potential of Allolobophora parva Eisen as a candidate for vermicomposting practices. Five organic waste mixtures: cow dung (CD), biogas plant slurry (BGS), cow dung+vegetable waste (CD+VW), BGS+VW and VW+Soil were vermicomposted using A. parva. Vermicomposting showed a decrease ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.07.004
更新日期：2009-12-01 00:00:00
abstract：:Production of polyhydroxyalkanoates (PHAs) in activated sludge treating wastewater represents an economical and environmental promising alternative to pure culture fermentations. A process for production of PHA from a paper mill wastewater was examined at laboratory scale. The three stage process examined consisted of...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.01.020
更新日期：2008-02-01 00:00:00
abstract：:The effect of electrodialytic treatment in terms of a current density, pH and Na(2)H(2)EDTA addition on the methanogenic activity of copper-amended anaerobic granular sludge taken from the UASB reactor from paper mill was evaluated. Moreover, the specific energy consumption and simplified operational and treatment cos...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.10.067
更新日期：2011-05-01 00:00:00
abstract：:In this study, two anaerobic membrane bioreactors (AnMBRs) were operated for 150days to treat bamboo industry wastewater (BIWW), and one of them was enhanced with bamboo charcoal (B-AnMBR). During the steady period, average chemical oxygen demand (COD) removal efficiencies of 94.5±2.9% and 89.1±3.1% were achieved in B...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.08.057
更新日期：2016-11-01 00:00:00
abstract：:Human milk fat substitutes (HMFs) with four kinds of n-3 fatty acid for infant formula were firstly synthesized using triacylglycerols (TAGs) from Nannochloropsis oculata rich in PA at the sn-2 position and free fatty acids (FFAs) from Isochrysis galbana rich in n-3 polyunsaturated fatty acids (n-3 PUFAs-ALA/SDA/DHA) ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.04.041
更新日期：2017-08-01 00:00:00
abstract：:This study investigated the performance of phosphogypsum and calcium magnesium phosphate fertilizer for nitrogen conservation during pig manure composting with cornstalk as the bulking agent. Results show that phosphogypsum increased nitrous oxide (N2O) emission, but significantly reduced ammonia (NH3) emission and th...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.07.172
更新日期：2018-02-01 00:00:00
abstract：:Streptomyces sp. GNDU 1 produced high levels of extra-cellular inulinase (0.552 IU/ml) after 24 h at pH 7.5, temperature 46 degrees C in the presence of 1% inulin. The optimum temperature and pH for enzyme activity were 60 degrees C and 5.5 respectively. Yeast extract as a nitrogen source was found to be most suitable...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/s0960-8524(02)00262-6
更新日期：2003-05-01 00:00:00
abstract：:Air pressure pulsation solid state fermentation (APP-SSF) was applied to produce feruloyl esterase (FAE) by Aspergillus niger. With the optimization of some variables by orthogonal design, the optimal condition obtained was 0.2 MPa (gauge pressure) of high pressure intensity, 30 min of low pressure duration and 20s of...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2008.08.032
更新日期：2009-02-01 00:00:00
abstract：:Direct hydrogenolysis of Kraft lignin was catalyzed over a series of supported Ni or Re catalysts in ethanol solvent. The best results showed that the oil yield of 96.70 wt% was obtained with less char formation at 330 °C for 3 h over 5Ni-5Re/Nb2O5 catalyst. Product analysis demonstrated that the monomer yield of 35.4...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.122582
更新日期：2020-03-01 00:00:00
abstract：:Bioelectrochemical treatment system (BET) with anoxic anodic microenvironment was studied with chemical wastewater (CW) in comparison with anoxic treatment (AxT, sequencing batch reactor (SBR)) with same parent anaerobic consortia. BET system documented relatively higher treatment efficiency at higher organic load (5....
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.10.028
更新日期：2014-01-01 00:00:00
abstract：:Rape straw pretreated by a continuous twin screw-driven reactor (CTSR) with hot water presented a distinctive particle-size distribution profile as a function of the operating temperature. The relative amount of finer particle size dramatically increased as the ratio of solid to liquid was increased. Size reduction th...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.12.077
更新日期：2013-02-01 00:00:00
abstract：:The Cel-BL11 gene from Paenibacillus campinasensis BL11 was cloned and expressed in Escherichia coli as a His-tag fusion protein. Zymographic analysis of the recombinant protein revealed cellulase activity corresponding to a protein with a 38-kDa molecular weight. The optimum temperature and pH for purified cellulase ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.05.043
更新日期：2010-10-01 00:00:00
abstract：:The present study evaluates the feasibility of increased power generation in microbial fuel cells (MFCs) coupled with acid elutriation fermentation. Raw piggery waste (RPW) and acid elutriation effluents (AEE) of piggery waste were used to generate bioelectricity in single-chambered air-cathode MFCs. RPW-fed MFCs exhi...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.08.021
更新日期：2017-11-01 00:00:00
abstract：:To investigate the existence of the asynchronism during the anaerobic co-digestion of different substrates, two typical substrates of food waste and corn stover were anaerobically digested with altering organic loadings (OL). The results indicated that the biodegradability of food waste and corn stover was calculated ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.04.074
更新日期：2014-08-01 00:00:00
abstract：:In order to explore changes in microbial enzyme activity and bacterial community, a 60-day composting experiment was conducted using cattle manure and straw under aeration rates of 0.45, 0.68, and 0.90 L min-1 kg-1 fresh weight. High aeration rate increased the cellulase, urease, alkaline and acid phosphatase activiti...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.122928
更新日期：2020-05-01 00:00:00
abstract：:Dissolved oxygen (DO) concentrations have often been shown to be important to decomposition rates of plant litter and thus may be a key factor in determining the supply of dissolved organic carbon (DOC) and carbon-dependent denitrification in wetlands. During the 2 months operation, DOC accumulation in anaerobic condi...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.10.122
更新日期：2011-02-01 00:00:00
abstract：:The study concerned the thermal oxidative degradation kinetics of agricultural residues, peanut shell (PS) and sunflower shell (SS). The thermal behaviors were evaluated via thermogravimetric analysis and the kinetic parameters were determined by using distributed activation energy model (DAEM) and global kinetic mode...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.04.047
更新日期：2018-08-01 00:00:00
abstract：:The progress of the conversion, the yield, the structure and the morphology of the produced carbonaceous materials as a function of time were systematically studied with pyrolysis-GC/FID and FESEM microscope. The conversion of galactoglucomannan, bleached kraft pulp and TEMPO oxidized cellulose nanofibrils followed th...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.04.061
更新日期：2016-07-01 00:00:00
