Abstract:
:This study analyzed the composition of a methane-generating microbial community and the corresponding active members during the transformation of three target substrates (food waste, cellulose or xylan) by barcoded 454 pyrosequencing of the bacterial and archaeal 16S rRNA genes in the DNA and RNA. The number of operational taxonomic units at 97% similarity for bacteria and archaea ranged from 162-261 and 31-166, respectively. Principal coordinates analysis and Venn diagram revealed that there were significant differences in the microbial community structure between the active members transforming each substrate and the inoculum. The active bacterial populations detected were those required for the hydrolysis of the amended substrate. The active archaeal populations were methanogens but the ratio of Methanosarcinales and Methanomicrobiales varied between the cultures. Overall, results of this study showed that a subset of the populations became active and altered in relative abundance during methane production according to the amended substrate.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Lu X,Rao S,Shen Z,Lee PKdoi
10.1016/j.biortech.2013.09.017subject
Has Abstractpub_date
2013-11-01 00:00:00pages
517-24eissn
0960-8524issn
1873-2976pii
S0960-8524(13)01440-5journal_volume
148pub_type
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