Effect of pretreatment and enzymatic hydrolysis of wheat straw on cell wall composition, hydrophobicity and cellulase adsorption.

Abstract:

:The present study aimed to determine the impact of cell wall composition and lignin content on enzyme adsorption and degradability. Thioacidolysis analysis of residual lignins in wheat straw after steam-explosion or organosolv pretreatment revealed an increase in lignin condensation degree of 27% and 33%, respectively. Surface hydrophobicity assessed through wettability tests decreased after the pretreatments (contact angle decrease of 20-50%), but increased with enzymatic conversion (30% maximum contact angle increase) and correlatively to lignin content. Adsorption of the three major cellulases Cel7A, Cel6A and Cel7B from Trichoderma reesei decreased with increasing hydrolysis time, down to 7%, 31% and 70% on the sample with the highest lignin content, respectively. The fraction of unspecifically bound enzymes was dependent both on the enzyme and the lignin content. Adsorption and specific activity were shown to be inversely proportional to lignin content and hydrophobicity, suggesting that lignin is one of the factors restricting enzymatic hydrolysis.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Heiss-Blanquet S,Zheng D,Lopes Ferreira N,Lapierre C,Baumberger S

doi

10.1016/j.biortech.2011.03.011

subject

Has Abstract

pub_date

2011-05-01 00:00:00

pages

5938-46

issue

10

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(11)00353-1

journal_volume

102

pub_type

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