Abstract:
:Bioaugmentation with hydrolytic microbes was applied to improve the methane yield of bioreactors fed with agricultural wastes. The efficiency of Clostridium thermocellum and Melioribacter roseus to degrade lignocellulosic matter was evaluated in batch and continuously stirred tank reactors (CSTRs). Results from batch assays showed that C. thermocellum enhanced the methane yield by 34%. A similar increase was recorded in CSTR during the bioaugmentation period; however, at steady-state the effect was noticeably lower (7.5%). In contrast, the bioaugmentation with M. roseus did not promote markedly the anaerobic biodegradability, as the methane yield was increased up to 10% in batch and no effect was shown in CSTR. High-throughput 16S rRNA amplicon sequencing was used to assess the effect of bioaugmentation strategies on bacterial and archaeal populations. The microbial analysis revealed that both strains were not markedly resided into biogas microbiome. Additionally, the applied strategies did not alter significantly the microbial communities.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Tsapekos P,Kougias PG,Vasileiou SA,Treu L,Campanaro S,Lyberatos G,Angelidaki Idoi
10.1016/j.biortech.2017.03.043subject
Has Abstractpub_date
2017-06-01 00:00:00pages
350-359eissn
0960-8524issn
1873-2976pii
S0960-8524(17)30313-9journal_volume
234pub_type
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