Abstract：
:The extracellular nuclease, NucB, from Bacillus licheniformis, can digest extracellular DNA in biofilms, causing biofilm dispersal, and may therefore be used commercially to remove biofilms. However, producing quantities of this secreted peptide is difficult and our aim was therefore to improve its laboratory scale production. This study builds on our understanding of B. licheniformis physiology to enhance NucB production. The addition of manganese, which triggers sporulation and enhances NucB expression, lead to a 5-fold increase in NucB production. Optimisation via Placket-Burman design of experiments identified 3 significant medium components and a subsequent Central Composite Design, to determine the optimum levels of these components, resulted in a 10-fold increase to 471U/ml. The optimal phosphate concentration was less than 0.3mM as this is known to inhibit nuclease production. The use of physiologically relevant information combined with optimisation represents a promising approach to increased enzyme production, which may also be widely applicable.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Rajarajan N,Ward AC,Burgess JG,Glassey Jdoi
10.1016/j.biortech.2012.12.064subject
Has Abstractpub_date
2013-02-01 00:00:00pages
552-8eissn
0960-8524issn
1873-2976pii
S0960-8524(12)01922-0journal_volume
130pub_type
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