Differential effects of nutrient limitations on biochemical constituents and docosahexaenoic acid production of Schizochytrium sp.

Abstract:

:Four nutrient limitation cultures, namely monosodium glutamate (MSG-L), phosphate (P-L), ammonium sulfate (NH4(+)-L) and double (D-L, MSG and P limitation) limited, were designed to study how cell growth and biochemical components of Schizochytrium sp. were affected by nutrient limitations. All limited conditions caused decrease in biomass especially MSG-L and D-L conditions. MSG-L condition attained the highest lipid yield of 30.73 g/l but the lowest protein content. P-L condition shortened the fermentation time and obtained the highest DHA productivity of 291 mg/lh. D-L condition was the most cost-effective fermentation condition which gained the highest input-output ratio. NH4(+)-L condition got the highest squalene and DHA content in lipids. Meanwhile, nitrogen limited conditions promoted the accumulation of neutral lipids. All limited conditions benefit the PUFAs accumulation in the neutral lipids. In addition, the existence of NH4(+) or the absence of MSG and phosphate reduced the unsaponifiable matters content in lipid.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Sun L,Ren L,Zhuang X,Ji X,Yan J,Huang H

doi

10.1016/j.biortech.2014.02.106

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

199-206

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(14)00277-6

journal_volume

159

pub_type

杂志文章
  • Nitrogen stress triggered biochemical and morphological changes in the microalgae Scenedesmus sp. CCNM 1077.

    abstract::The aim of present study was to investigate the effects of nitrogen limitation as well as sequential nitrogen starvation on morphological and biochemical changes in Scenedesmus sp. CCNM 1077. The results revealed that the nitrogen limitation and sequential nitrogen starvation conditions significantly decreases the pho...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2014.01.025

    authors: Pancha I,Chokshi K,George B,Ghosh T,Paliwal C,Maurya R,Mishra S

    更新日期:2014-03-01 00:00:00

  • Co-pelletizing characteristics of torrefied wheat straw with peanut shell.

    abstract::The co-pelletizing characteristics of torrefied wheat straw and peanut shell with adding water were investigated. The physicochemical and friction characteristics of biochar were determined to investigate the mechanism of biochar inter-particle cohesive bonding. Results showed that optimized process conditions were ob...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2017.02.091

    authors: Bai X,Wang G,Gong C,Yu Y,Liu W,Wang D

    更新日期:2017-06-01 00:00:00

  • Enhancement of cell biomass and cell activity of astaxanthin-rich Haematococcus pluvialis.

    abstract::Fed-batch culture and the transformation conditions of Haematococcus pluvialis in a 5L photobioreactor were investigated. Methods of feeding model, low temperature at night and proper feeding time were used to increase both cell biomass and cell activity. Dry cell weight of 1.87 g/L which was 2.0-fold of batch culture...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2015.02.101

    authors: Sun H,Kong Q,Geng Z,Duan L,Yang M,Guan B

    更新日期:2015-06-01 00:00:00

  • Operation strategy for constructed wetlands in dry seasons with insufficient influent wastewater.

    abstract::Using vertical flow constructed wetlands (VFCWs) with different influent wastewater volumes and feeding modes, this study aimed to identify the optimal operation strategy for dry seasons under wastewater deficiency. Using half the influent wastewater volume (HIWV) did not necessarily improve the removal efficiency (RE...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2020.124049

    authors: Zhang M,Chen C,Zhou S,Yang J,Qiu H,Zhao D,An S

    更新日期:2020-12-01 00:00:00

  • Sub-supercritical liquefaction of rice stalk for the production of bio-oil: Effect of solvents.

    abstract::The effect of solvents (water and ethanol) on liquefaction characteristics of rice stalk (RS) was investigated in an autoclave. The highest conversion and liquid yield in water and ethanol were 84.95 wt%, 72.62 wt% and 78.93wt%, 63.84 wt%, respectively. FTIR and GC-MS of the bio-oils obtained from subcritical water (S...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2015.08.088

    authors: Li R,Li B,Yang T,Kai X,Wang W,Jie Y,Zhang Y,Chen G

    更新日期:2015-12-01 00:00:00

  • Mechanism and challenges in commercialisation of algal biofuels.

    abstract::Biofuels made from algal biomass are being considered as the most suitable alternative energy in current global and economical scenario. Microalgae are known to produce and accumulate lipids within their cell mass which is similar to those found in many vegetable oils. The efficient lipid producer algae cell mass has ...

    journal_title:Bioresource technology

    pub_type: 杂志文章,评审

    doi:10.1016/j.biortech.2010.06.057

    authors: Singh A,Nigam PS,Murphy JD

    更新日期:2011-01-01 00:00:00

  • Influence of organic loading rate and solid retention time on polyhydroxybutyrate production from hybrid poplar hydrolysates using mixed microbial cultures.

    abstract::The aim of this study was to investigate the potential of using wood hydrolysates (enzymatically hydrolyzed from hybrid poplar) as substrate to produce polyhydroxybutyrate (PHB) using mixed microbial cultures. The optimal operational conditions for fed-batch bioreactors were 4d solid retention time with an organic loa...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2014.10.049

    authors: Dai J,Gliniewicz K,Settles ML,Coats ER,McDonald AG

    更新日期:2015-01-01 00:00:00

  • Process enhancement of supercritical methanol biodiesel production by packing beds.

    abstract::Continuous fixed bed reactors filled by three kinds of packing which were glass bead, glass spring and Dixon rings were investigated. The effect of temperature, pressure, the molar ratio of methanol to oil, flow rate, the size and shape of the packing were researched. The highest yield 90.84% of FAME was obtained by f...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.12.085

    authors: Qiao BQ,Zhou D,Li G,Yin JZ,Xue S,Liu J

    更新日期:2017-03-01 00:00:00

  • Optimization of microwave-assisted calcium chloride pretreatment of corn stover.

    abstract::A 62.5% (w/w) CaCl(2) solution was used in the microwave pretreatment of corn stover. The central composite design (CCD) of response surface methodology (RSM) was employed to design and optimize the CaCl(2)-assisted microwave pretreatment (CaCl(2)-pretreatment). Temperature and time were the main factors affecting the...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2012.09.114

    authors: Li H,Xu J

    更新日期:2013-01-01 00:00:00

  • Purification and characterization of angiotensin I converting enzyme inhibitory peptides from the rotifer, Brachionus rotundiformis.

    abstract::Angiotensin I converting enzyme (ACE) inhibitory peptide was isolated from the marine rotifer, Brachionus rotundiformis. ACE inhibitory peptides were separated from rotifer hydrolysate prepared by Alcalase, alpha-chymotrypsin, Neutrase, papain, and trypsin. The Alcalase hydrolysate had the highest ACE inhibitory activ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2009.05.057

    authors: Lee JK,Hong S,Jeon JK,Kim SK,Byun HG

    更新日期:2009-11-01 00:00:00

  • Fatty acid ethyl esters production in aqueous phase by the oleaginous yeast Rhodosporidium toruloides.

    abstract::Fatty acid ethyl esters (FAEEs) are attractive biofuel molecules. Conventional FAEEs production process uses triglycerides and ethanol as feedstocks and is sensitive to water contents. In this work, we show that the oleaginous yeast Rhodosporidium toruloides cells are capable of converting lipids into FAEEs intracellu...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2013.10.023

    authors: Jin G,Zhang Y,Shen H,Yang X,Xie H,Zhao ZK

    更新日期:2013-12-01 00:00:00

  • Biosurfactant production by Aureobasidium pullulans in stirred tank bioreactor: New approach to understand the influence of important variables in the process.

    abstract::Surfactants are amphiphilic molecules with large industrial applications produced currently by chemical routes mainly derived from oil industry. However, biotechnological process, aimed to develop new sustainable process configurations by using favorable microorganisms, already requires investigations in more details....

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2017.06.088

    authors: Brumano LP,Antunes FAF,Souto SG,Dos Santos JC,Venus J,Schneider R,da Silva SS

    更新日期:2017-11-01 00:00:00

  • Streptomyces griseorubens mediated delignification of paddy straw for improved enzymatic saccharification yields.

    abstract::Biological pretreatment of paddy straw was carried out using an actinomycete isolate, identified as Streptomyces griseorubens ssr38, for delignification under solid state fermentation and enhanced sugar recovery by enzymatic saccharification. After 10 days incubation, the inoculated paddy straw was extracted with mild...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2012.11.040

    authors: Saritha M,Arora A,Singh S,Nain L

    更新日期:2013-05-01 00:00:00

  • Assessing the efficiency of urban waste biocomposting by analytical pyrolysis (Py-GC/MS).

    abstract::Analytical pyrolysis (Py-GC/MS) was used to study complex composting processes. The technique was first validated for reproducibility and finally applied to assess the efficiency of a microbial bio-accelerator product (CBB) in composting organic residues with different composition. Fresh lignocellulosic and urban wast...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2008.06.067

    authors: González-Vila FJ,González-Pérez JA,Akdi K,Gómis MD,Pérez-Barrera F,Verdejo T

    更新日期:2009-02-01 00:00:00

  • Bioremediation of pulp and paper mill effluent by a novel fungal consortium isolated from polluted soil.

    abstract::Two basidiomycetous fungi (Merulius aureus syn. Phlebia sp. and an unidentified genus) and a deuteromycetous fungus (Fusarium sambucinum Fuckel MTCC 3788) were isolated from soils affected with effluents of a pulp and paper mill over several years. These isolates were immobilized on nylon mesh and the consortium was u...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2006.11.021

    authors: Malaviya P,Rathore VS

    更新日期:2007-12-01 00:00:00

  • Characterization of changes in viscosity and insoluble solids content during enzymatic saccharification of pretreated corn stover slurries.

    abstract::Viscosity trends in pretreated corn stover slurries undergoing enzymatic saccharification were characterized for a range of initial insoluble solids concentrations from 10% to 25% and correlated with total glucose released and changes in insoluble solids concentration throughout a 7day period. Viscosity trends are def...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2009.12.071

    authors: Dunaway KW,Dasari RK,Bennett NG,Eric Berson R

    更新日期:2010-05-01 00:00:00

  • Comparative studies on removal of Congo red by native and modified mycelial pellets of Trametes versicolor in various reactor modes.

    abstract::Aerated and rotated mode adsorption experiments were carried out for the removal of Congo red from aqueous solution using native and pre-treated mycelial pellets/biomass of Trametes versicolor. The effect of process parameters like contact time, dosage of adsorbent, adsorbate concentration and pH on adsorption was inv...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2007.02.022

    authors: Binupriya AR,Sathishkumar M,Swaminathan K,Kuz CS,Yun SE

    更新日期:2008-03-01 00:00:00

  • Performance of a fungal monolith bioreactor for the removal of styrene from polluted air.

    abstract::Gas-phase styrene removal using the fungus, Sporothrix variecibatus was evaluated in a novel monolith bioreactor, receiving a continuous supply of nutrients from the trickling liquid phase. During the start-up process, the monolith reactor was operated for 22 days with relatively low styrene concentrations in the gas-...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2009.10.060

    authors: Rene ER,López ME,Veiga MC,Kennes C

    更新日期:2010-04-01 00:00:00

  • The significance of biomass in a circular economy.

    abstract::A circular economy relies on the value of resources being maximised indefinitely, requiring that virtually no unrecoverable waste occurs. Biomass is highly significant in a circular economy in terms of material products and the provision of energy. To establish a circular bioeconomy, the practical implications of biom...

    journal_title:Bioresource technology

    pub_type: 杂志文章,评审

    doi:10.1016/j.biortech.2020.122755

    authors: Sherwood J

    更新日期:2020-03-01 00:00:00

  • New functional biocarriers for enhancing the performance of a hybrid moving bed biofilm reactor-membrane bioreactor system.

    abstract::In this study, new sponge modified plastic carriers for moving bed biofilm reactor (MBBR) was developed. The performance and membrane fouling behavior of a hybrid MBBR-membrane bioreactor (MBBR-MBR) system were also evaluated. Comparing to the MBBR with plastic carriers (MBBR), the MBBR with sponge modified biocarrier...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.02.057

    authors: Deng L,Guo W,Ngo HH,Zhang X,Wang XC,Zhang Q,Chen R

    更新日期:2016-05-01 00:00:00

  • Synergistic biorefinery of Scenedesmus obliquus and Ulva lactuca in poultry manure towards sustainable bioproduct generation.

    abstract::Exploiting solar energy for growing algal biomass in waters enriched with farm manures is a holistic method of waste management. The proposed cultivation strategy termed SAR'CENA ('Synergistic Algal Refinery for Circular Economy using Nutrient Analogues), involves integrated cultivation of microalga, Scenedesmus obliq...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2019.122462

    authors: Agarwal A,Mhatre A,Pandit R,Lali AM

    更新日期:2020-02-01 00:00:00

  • Cloning and expression of cellulase gene EG1 from Rhizopus stolonifer var. reflexus TP-02 in Escherichia coli.

    abstract::A novel gene, EG encoding enzymes involved in carboxymethyl cellulose (CMC) degradation was isolated, sequenced from the filamentous fungus Rhizopus stolonifer var. reflexus TP-02, and expressed in Escherichia coli BL21. The results showed that the gene amplified from the cDNA of the strain could be classified as the ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2009.06.091

    authors: Tang B,Pan H,Zhang Q,Ding L

    更新日期:2009-12-01 00:00:00

  • Maximizing enzymatic hydrolysis efficiency of bamboo with a mild ethanol-assistant alkaline peroxide pretreatment.

    abstract::To overcome the delignification saturation point in traditional alkaline hydrogen peroxide pretreatment (AHP), a powerful modified AHP delignification methodology was established by introducing ethanol into the system. The pretreatment caused significant lignin removal of bamboo at elevated pretreatment temperature wi...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2019.122568

    authors: Huang C,Fang G,Yu L,Zhou Y,Meng X,Deng Y,Shen K,Ragauskas AJ

    更新日期:2020-03-01 00:00:00

  • Engineering aspects of hydrothermal pretreatment: From batch to continuous operation, scale-up and pilot reactor under biorefinery concept.

    abstract::Different pretreatments strategies have been developed over the years mainly to enhance enzymatic cellulose degradation. In the new biorefinery era, a more holistic view on pretreatment is required to secure optimal use of the whole biomass. Hydrothermal pretreatment technology is regarded as very promising for lignoc...

    journal_title:Bioresource technology

    pub_type: 杂志文章,评审

    doi:10.1016/j.biortech.2019.122685

    authors: Ruiz HA,Conrad M,Sun SN,Sanchez A,Rocha GJM,Romaní A,Castro E,Torres A,Rodríguez-Jasso RM,Andrade LP,Smirnova I,Sun RC,Meyer AS

    更新日期:2020-03-01 00:00:00

  • Influence of the reaction conditions on the enzyme catalyzed transesterification of castor oil: A possible step in biodiesel production.

    abstract::The identification of the influence of the reaction parameters is of paramount importance when defining a process design. In this work, non-edible castor oil was reacted with methanol to produce a possible component for biodiesel blends, using liquid enzymes as the catalyst. Temperature, alcohol-to-oil molar ratio, en...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2017.06.118

    authors: Andrade TA,Errico M,Christensen KV

    更新日期:2017-11-01 00:00:00

  • Arabinose substitution degree in xylan positively affects lignocellulose enzymatic digestibility after various NaOH/H2SO4 pretreatments in Miscanthus.

    abstract::Xylans are the major hemicelluloses in grasses, but their effects on biomass saccharification remain unclear. In this study, we examined the 79 representative Miscanthus accessions that displayed a diverse cell wall composition and varied biomass digestibility. Correlation analysis showed that hemicelluloses level has...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2012.12.107

    authors: Li F,Ren S,Zhang W,Xu Z,Xie G,Chen Y,Tu Y,Li Q,Zhou S,Li Y,Tu F,Liu L,Wang Y,Jiang J,Qin J,Li S,Li Q,Jing HC,Zhou F,Gutterson N,Peng L

    更新日期:2013-02-01 00:00:00

  • Influence of airflow intensity on phytase production by solid-state fermentation.

    abstract::Phytase production by Aspergillus niger F3 by solid state fermentation (SSF) on citrus peel was evaluated at pilot scale under different aeration conditions. The best airflow intensity was 1 VkgM (Lair kg medium(-1) min(-1)), which allowed to produce 65 units of phytases per gram in dry basis (65 Ug(-1) d.b.) as it re...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2012.05.032

    authors: Rodríguez-Fernández DE,Rodríguez-León JA,de Carvalho JC,Karp SG,Sturm W,Parada JL,Soccol CR

    更新日期:2012-08-01 00:00:00

  • A microalgae residue based carbon solid acid catalyst for biodiesel production.

    abstract::Biodiesel production from microalgae is recognized as one of the best solutions to deal with the energy crisis issues. However, after the oil extraction from the microalgae, the microalgae residue was generally discarded or burned. Here a novel carbon-based solid acid catalyst derived from microalgae residue by in sit...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2013.07.117

    authors: Fu X,Li D,Chen J,Zhang Y,Huang W,Zhu Y,Yang J,Zhang C

    更新日期:2013-10-01 00:00:00

  • Chemical, structural and combustion characteristics of carbonaceous products obtained by hydrothermal carbonization of palm empty fruit bunches.

    abstract::A carbon-rich solid product, denoted as hydrochar, was synthesized by hydrothermal carbonization (HTC) of palm oil empty fruit bunch (EFB), at different pre-treatment temperatures of 150, 250 and 350 °C. The conversion of the raw biomass to its hydrochar occurred via dehydration and decarboxylation processes. The hydr...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2012.09.042

    authors: Parshetti GK,Kent Hoekman S,Balasubramanian R

    更新日期:2013-05-01 00:00:00

  • Biotechnological applications of inulin-rich feedstocks.

    abstract::Inulin is a naturally occurring second largest storage polysaccharide with a wide range of applications in pharmaceutical and food industries. It is a robust polysaccharide which consists of a linear chain of β-2, 1-linked-d-fructofuranose molecules terminated with α-d-glucose moiety at the reducing end. It is present...

    journal_title:Bioresource technology

    pub_type: 杂志文章,评审

    doi:10.1016/j.biortech.2018.11.031

    authors: Singh RS,Singh T,Larroche C

    更新日期:2019-02-01 00:00:00