Abstract：
:The aim of this study was to investigate the potential of using wood hydrolysates (enzymatically hydrolyzed from hybrid poplar) as substrate to produce polyhydroxybutyrate (PHB) using mixed microbial cultures. The optimal operational conditions for fed-batch bioreactors were 4d solid retention time with an organic loading rate of 2.5g/Ld. The maximum PHB accumulated was 27% of cell dry weight with a yield of 0.32g/g (g PHB produced per g sugars consumed). Microbial community analysis was done at the genus level by 16S rRNA sequencing on an Illumina system and community evolution was observed among different samples and initial seed. Actinobacteria, Alpha- and Beta-proteobacteria were found to be the dominant groups in all the bioreactors. Several PHB-storing microorganisms were characterized belonging to Alpha- and Beta-proteobacteria.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Dai J,Gliniewicz K,Settles ML,Coats ER,McDonald AGdoi
10.1016/j.biortech.2014.10.049subject
Has Abstractpub_date
2015-01-01 00:00:00pages
23-33eissn
0960-8524issn
1873-2976pii
S0960-8524(14)01471-0journal_volume
175pub_type
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