Cloning and expression of cellulase gene EG1 from Rhizopus stolonifer var. reflexus TP-02 in Escherichia coli.

Abstract:

:A novel gene, EG encoding enzymes involved in carboxymethyl cellulose (CMC) degradation was isolated, sequenced from the filamentous fungus Rhizopus stolonifer var. reflexus TP-02, and expressed in Escherichia coli BL21. The results showed that the gene amplified from the cDNA of the strain could be classified as the family of endoglucanase. During the fermentation process, the maximum endoglucanase activity (i.e. 0.715 IU/ml) of the recombinant bacteria was obtained at 36 h. The SDS-PAGE analysis on purified samples showed that a band with apparent molecular weight of about 40 kDa was detected after staining with Coomassie brilliant blue.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Tang B,Pan H,Zhang Q,Ding L

doi

10.1016/j.biortech.2009.06.091

subject

Has Abstract

pub_date

2009-12-01 00:00:00

pages

6129-32

issue

23

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(09)00692-0

journal_volume

100

pub_type

杂志文章
  • Thermal pyrolysis characteristics of macroalgae Cladophora glomerata.

    abstract::The Cladophora glomerata (C. glomerata) is a kind of widely distributed macroalgae in the freshwater ecosystems. It primarily consists of carbohydrates that can be converted into biofuel by pyrolysis. In this study, thermogravimetric analysis (TGA) was used to investigate the thermal behavior and kinetics of C. glomer...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2017.06.041

    authors: Gao W,Chen K,Zeng J,Xu J,Wang B

    更新日期:2017-11-01 00:00:00

  • Hydrothermal carbonization of Opuntia ficus-indica cladodes: Role of process parameters on hydrochar properties.

    abstract::Opuntia ficus-indica cladodes are a potential source of solid biofuel from marginal, dry land. Experiments assessed the effects of temperature (180-250°C), reaction time (0.5-3h) and biomass to water ratio (B/W; 0.07-0.30) on chars produced via hydrothermal carbonization. Multivariate linear regression demonstrated th...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2017.09.072

    authors: Volpe M,Goldfarb JL,Fiori L

    更新日期:2018-01-01 00:00:00

  • Upgrading the rice husk char obtained by flash pyrolysis for the production of amorphous silica and high quality activated carbon.

    abstract::The overall valorization of rice husk char obtained by flash pyrolysis in a conical spouted bed reactor (CSBR) has been studied in a two-step process. Thus, silica has been recovered in a first step and the remaining carbon material has been subjected to steam activation. The char samples used in this study have been ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2014.07.073

    authors: Alvarez J,Lopez G,Amutio M,Bilbao J,Olazar M

    更新日期:2014-10-01 00:00:00

  • Symbiotic relationship between hydrothermal carbonization technology and anaerobic digestion for food waste in China.

    abstract::Food waste (FW) is traditionally disposed through landfills and incineration in China. Nowadays, there are some promising methods, such as anaerobic digestion (AD) or feeding and composting, which are being applied in pilot cities. However, the inherent characteristics of Chinese FW may be regarded as a double-edged s...

    journal_title:Bioresource technology

    pub_type: 杂志文章,评审

    doi:10.1016/j.biortech.2018.03.102

    authors: Zhou Y,Engler N,Nelles M

    更新日期:2018-07-01 00:00:00

  • A novel assay for rapid in vivo determination of phenotypic stability of recombinant ethanol-producing microorganisms.

    abstract::A rapid empirical assay is presented for assessing the phenotypic stability of continuous cultures of recombinant bacteria containing transposed pdc and adh genes for ethanol production. The method measures spectrophotometrically the rate of colour formation when cells oxidize added ethanol to acetaldehyde in the pres...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2009.01.013

    authors: Zhou B,Martin GJ,Pamment NB

    更新日期:2009-06-01 00:00:00

  • Co-digestion of food and garden waste with mixed sludge from wastewater treatment in continuously stirred tank reactors.

    abstract::Co-digestions of urban organic waste were conducted to investigate the effect of the mixing ratio between sludge, food waste, grass clippings and green waste at different hydraulic retention times (HRTs). Compared to the digestion of 100% sludge, the methane yield increased by 48% and 35%, when co-digesting sludge wit...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.01.085

    authors: Fitamo T,Boldrin A,Boe K,Angelidaki I,Scheutz C

    更新日期:2016-04-01 00:00:00

  • Eco-efficiency assessment of farm-scaled biogas plants.

    abstract::The aim of this study was to analyse the eco-efficiency of 15 agricultural biogas plants located in Northern Italy. For this, the combination of life cycle assessment (LCA) and data envelopment analysis (DEA) methodologies was considered with the purpose of identifying efficient operational plants and proposing improv...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2017.01.055

    authors: Lijó L,Lorenzo-Toja Y,González-García S,Bacenetti J,Negri M,Moreira MT

    更新日期:2017-08-01 00:00:00

  • Comparison of nitrite accumulation performance and microbial community structure in endogenous partial denitrification process with acetate and glucose served as carbon source.

    abstract::Although the combination of endogenous partial denitrification (EPD) and Anammox (EPD-AMX) were developed for deep-level nitrogen removal, the effects of different carbon source were not clear. In this study, the EPD performance was investigated comparatively with acetate (EPDA) and glucose (EPDG). Results revealed th...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2020.124405

    authors: Chu G,Yu D,Wang X,Wang Q,He T,Zhao J

    更新日期:2021-01-01 00:00:00

  • Improvement of biomass properties by pretreatment with ionic liquids for bioconversion process.

    abstract::Cassava pulp residue and rice straw were used as a precursor for pretreatment with ionic liquids to study the effects of pretreatment conditions on product yield and properties. Cassava pulp residue is a potential biomass in the bioconversion process due to it requiring mild pretreatment conditions while providing a h...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2012.02.023

    authors: Weerachanchai P,Leong SS,Chang MW,Ching CB,Lee JM

    更新日期:2012-05-01 00:00:00

  • Metabolic engineering of Klebsiella oxytoca M5a1 to produce optically pure D-lactate in mineral salts medium.

    abstract::Klebsiella oxytoca strains were constructed to produce optical pure d-lactate by pH-controlled batch fermentation in mineral salts medium. The alcohol dehydrogenase gene, adhE, and the phospho-transacetylase/acetate kinase A genes, pta-ackA, were deleted from the wild type. KMS002 (ΔadhE) and KMS004 (ΔadhE Δpta-ackA) ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2012.05.114

    authors: Sangproo M,Polyiam P,Jantama SS,Kanchanatawee S,Jantama K

    更新日期:2012-09-01 00:00:00

  • An efficient production of high-pure xylooligosaccharides from corncob with affinity adsorption-enzymatic reaction integrated approach.

    abstract::Xylooligosaccharides (XOS) are high value-added ingredients for functional foods and they have potential use as prebiotics. In order to reduce the production cost of XOS, we constructed a fusion enzyme consisting of expansin and endo-xylanase from B. subtilis Lucky9 to produce high-pure XOS with affinity adsorption-en...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2017.06.002

    authors: Chang S,Chu J,Guo Y,Li H,Wu B,He B

    更新日期:2017-10-01 00:00:00

  • Production of 2-keto-L-gulonic acid by metabolically engineered Escherichia coli.

    abstract::The 2-keto-L-gulonic acid (2-KLG) is the direct precursor for industrial vitamin C production. The main biosynthetic method for 2-KLG production is the classical two-step fermentation route. However, disadvantages of this method are emerging, including high consumption of energy, difficulties in strain screening, comp...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2020.124069

    authors: Zeng W,Wang P,Li N,Li J,Chen J,Zhou J

    更新日期:2020-12-01 00:00:00

  • Formation and long-term stability of nitrifying granules in a sequencing batch reactor.

    abstract::The formation and long-term stability of nitrifying granules in a sequencing batch reactor was investigated in this study. The results showed that nitrifying granules with a size of 240 microm and SVI of 40 ml g(-1) were formed on day 21 at a settling time of 10 min. Maintaining settling time at 15 min from day 57 to ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2007.07.041

    authors: Liu YQ,Wu WW,Tay JH,Wang JL

    更新日期:2008-06-01 00:00:00

  • Conversion of waste produced by the deodorization of palm oil as feedstock for the production of biodiesel using a catalyst prepared from waste material.

    abstract::The distillate produced by deodorization of palm oil (DDPO) is a waste that corresponds to 4% of the product formed in this process. DDPO is 83% free of fatty acids (FFA), making it a good material for biodiesel production. In this paper, a catalyst prepared from a waste material, Amazon flint kaolin, was used for the...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2011.06.004

    authors: do Nascimento LA,Angélica RS,da Costa CE,Zamian JR,da Rocha Filho GN

    更新日期:2011-09-01 00:00:00

  • Membrane fouling mechanism in ultrafiltration of succinic acid fermentation broth.

    abstract::The membrane fouling mechanism was studied in treating succinic acid fermentation broth during dead-end ultrafiltration. Different membranes were used and two models were applied to analyze the fouling mechanism. Resistance-in-series model was applied to determine the main factor that caused the operation resistance. ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2012.03.099

    authors: Wang C,Li Q,Tang H,Yan D,Zhou W,Xing J,Wan Y

    更新日期:2012-07-01 00:00:00

  • Control of partial nitrification using pulse aeration for treating digested effluent of swine wastewater.

    abstract::Three sequencing batch reactors (SBRs) were used to investigate the influence of pulse frequencies on the partial nitrification (PN) process in this study. At a total aeration time of 6 min each hour, the aerated frequencies of R1, R2 and R3 were 6, 3 and 2 time h-1. During the steady period (117-143d), the nitrite ac...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2018.04.084

    authors: Wang S,Deng L,Zheng D,Wang L,Zhang Y,Yang H,Jiang Y,Huang F

    更新日期:2018-08-01 00:00:00

  • Thermo- and mesophilic anaerobic digestion of wheat straw by the upflow anaerobic solid-state (UASS) process.

    abstract::In this experimental work, the feasibility of wheat straw as a feedstock for biogas production is investigated using the newly developed upflow anaerobic solid-state (UASS) process. With the analytical emphasis placed on methane and metabolite production, both mesophilic and thermophilic 39 L UASS reactors were operat...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2012.08.063

    authors: Pohl M,Mumme J,Heeg K,Nettmann E

    更新日期:2012-11-01 00:00:00

  • Production and recovery of an alkaline exo-polygalacturonase from Bacillus subtilis RCK under solid-state fermentation using statistical approach.

    abstract::The empirical models developed through two independent RSM (RSM-I, 2(3); RSM-II, 2(5)) in terms of effective operational factors of inoculum age, inoculum volume, wheat bran-to-moisture ratio (RSM-I) and contact time, extraction temperature, agitation, fermented bran-to-solvent ratio and SDS (RSM-II) were found adequa...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2007.03.009

    authors: Gupta S,Kapoor M,Sharma KK,Nair LM,Kuhad RC

    更新日期:2008-03-01 00:00:00

  • Impact of copper on the diazotroph abundance and community composition during swine manure composting.

    abstract::Biological nitrogen fixation is a major pathway in ecosystems. This study investigated the effects of adding Cu at different levels (0, 200, and 2000 mg kg-1) on the diazotroph community during swine manure composting. Quantitative PCR and high-throughput sequencing were used to analyze the abundances of diazotrophs a...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2018.01.120

    authors: Yin Y,Gu J,Wang X,Zhang K,Hu T,Ma J,Wang Q

    更新日期:2018-05-01 00:00:00

  • Electro-biocatalytic conversion of carbon dioxide to alcohols using gas diffusion electrode.

    abstract::Impact of gas diffusion electrodes (GDEs) was evaluated in enhancing the CO2 bio-availability for its transformation to C4-organics, especially to alcohols using selective mixed culture. Observed current density was more stable (9-11 A/m2) than submerged experiments reported and significantly varied with pH and respec...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2018.02.058

    authors: Srikanth S,Singh D,Vanbroekhoven K,Pant D,Kumar M,Puri SK,Ramakumar SSV

    更新日期:2018-10-01 00:00:00

  • Substrate induced emergence of different active bacterial and archaeal assemblages during biomethane production.

    abstract::This study analyzed the composition of a methane-generating microbial community and the corresponding active members during the transformation of three target substrates (food waste, cellulose or xylan) by barcoded 454 pyrosequencing of the bacterial and archaeal 16S rRNA genes in the DNA and RNA. The number of operat...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2013.09.017

    authors: Lu X,Rao S,Shen Z,Lee PK

    更新日期:2013-11-01 00:00:00

  • Hydrophilicity/hydrophobicity of anaerobic granular sludge surface and their causes: An in situ research.

    abstract::The pursuit of high-rate anaerobic reactor has powerfully promoted the studies of anaerobic granular sludge (AnGS) recently. In this work, Methylene Blue and Rhodamine B were used as hydrophilic/hydrophobic indicators to investigate the hydrophilicity/hydrophobicity of AnGS, and several analysis methods were applied t...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.08.012

    authors: Li Y,Zheng P,Zhang M,Zeng Z,Wang Z,Ding A,Ding K

    更新日期:2016-11-01 00:00:00

  • Stability of autotrophic nitrogen removal system under four non-steady operations.

    abstract::Stable nitrogen removal from the digester supernatant for sludge via the nitritation-anammox process under steady operations of ammonium concentration and flow rate has been often reported. In this study, the effects of four non-steady operations, intentional fluctuations of influent concentration from 890 to 650 mg-N...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2013.03.130

    authors: Kimura Y,Itokawa H,Noto K,Murakami T,Isaka K

    更新日期:2013-06-01 00:00:00

  • Application of calcined waste fish (Labeo rohita) scale as low-cost heterogeneous catalyst for biodiesel synthesis.

    abstract::This paper explores the feasibility of converting waste Rohu fish (Labeo rohita) scale into a high-performance, reusable, low-cost heterogeneous catalyst for synthesis of biodiesel from soybean oil. The thermo-gravimetric analysis (TGA) and X-ray diffraction (XRD) analysis revealed that a significant portion of the ma...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2010.10.123

    authors: Chakraborty R,Bepari S,Banerjee A

    更新日期:2011-02-01 00:00:00

  • Bioethanol fermentation of concentrated rice straw hydrolysate using co-culture of Saccharomyces cerevisiae and Pichia stipitis.

    abstract::Rice straw is one of the abundant lignocellulosic feed stocks in the world and has been selected for producing ethanol at an economically feasible manner. It contains a mixture of sugars (hexoses and pentoses). Biphasic acid hydrolysis was carried out with sulphuric acid using rice straw. After acid hydrolysis, the su...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2011.03.019

    authors: Yadav KS,Naseeruddin S,Prashanthi GS,Sateesh L,Rao LV

    更新日期:2011-06-01 00:00:00

  • Multiple linear regression model for predicting biomass digestibility from structural features.

    abstract::A total of 147 model lignocellulose samples with a broad spectrum of structural features (lignin contents, acetyl contents, and crystallinity indices) were hydrolyzed with a wide range of cellulase loadings during 1-, 6-, and 72-h hydrolysis periods. Carbohydrate conversions at 1, 6, and 72 h were linearly proportiona...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2009.11.034

    authors: Zhu L,O'Dwyer JP,Chang VS,Granda CB,Holtzapple MT

    更新日期:2010-07-01 00:00:00

  • Phosphoric acid-activated wood biochar for catalytic conversion of starch-rich food waste into glucose and 5-hydroxymethylfurfural.

    abstract::The catalytic activity of engineered biochar was scrutinized for generation of glucose and hydroxymethylfurfural (HMF) from starch-rich food waste (bread, rice, and spaghetti). The biochar catalysts were synthesized by chemical activation of pinewood sawdust with phosphoric acid at 400-600 °C. Higher activation temper...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2018.07.048

    authors: Cao L,Yu IKM,Tsang DCW,Zhang S,Ok YS,Kwon EE,Song H,Poon CS

    更新日期:2018-11-01 00:00:00

  • Improving docosahexaenoic acid productivity of Schizochytrium sp. by a two-stage AEMR/shake mixed culture mode.

    abstract::In this work, an aeration-enhanced membrane reactor (AEMR) was built to control dissolved oxygen in Schizochytrium sp. broth. The effect of culture modes, i.e. single shake and AEMR mode, on the docosahexaenoic acid (DHA) productivity of Schizochytrium sp. was investigated. Experimental results showed that the biomass...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2013.05.072

    authors: Zhang L,Zhao H,Lai Y,Wu J,Chen H

    更新日期:2013-08-01 00:00:00

  • Enrichment of high activity nitrifers to enhance partial nitrification process.

    abstract::Partial nitrification (PN) process is an essential pre-treatment to Anammox process for nitrogen removal from ammonium-rich wastewaters. High activity nitrifiers (HANs) are considered as the key to high-rate PN process. The HANs could be successfully enriched at free ammonia (FA) concentrations of 10-20 mg NH(3)-NL(-1...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2010.04.080

    authors: Chen J,Zheng P,Yu Y,Mahmood Q,Tang C

    更新日期:2010-10-01 00:00:00

  • Effective harvesting of the marine microalga Thalassiosira pseudonana by Marinobacter sp. FL06.

    abstract::In this study, Marinobacter sp. FL06 was used to effectively harvest the energy-producing microalga Thalassiosira pseudonana through direct flocculation. Strain FL06 showed 92.7% flocculating efficiency against T. pseudonana, and no metal ion was added for the flocculation process, resulting in a more environmentally ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2018.08.077

    authors: Lei X,Zheng W,Ding H,Zhu X,Chen Q,Xu H,Zheng T,Tian Y

    更新日期:2018-12-01 00:00:00