Abstract:
:A novel gene, EG encoding enzymes involved in carboxymethyl cellulose (CMC) degradation was isolated, sequenced from the filamentous fungus Rhizopus stolonifer var. reflexus TP-02, and expressed in Escherichia coli BL21. The results showed that the gene amplified from the cDNA of the strain could be classified as the family of endoglucanase. During the fermentation process, the maximum endoglucanase activity (i.e. 0.715 IU/ml) of the recombinant bacteria was obtained at 36 h. The SDS-PAGE analysis on purified samples showed that a band with apparent molecular weight of about 40 kDa was detected after staining with Coomassie brilliant blue.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Tang B,Pan H,Zhang Q,Ding Ldoi
10.1016/j.biortech.2009.06.091subject
Has Abstractpub_date
2009-12-01 00:00:00pages
6129-32issue
23eissn
0960-8524issn
1873-2976pii
S0960-8524(09)00692-0journal_volume
100pub_type
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