Abstract:
:Xylooligosaccharides (XOS) are high value-added ingredients for functional foods and they have potential use as prebiotics. In order to reduce the production cost of XOS, we constructed a fusion enzyme consisting of expansin and endo-xylanase from B. subtilis Lucky9 to produce high-pure XOS with affinity adsorption-enzymatic reaction integrated approach. By optimization of inserting linker, the specific xylanase activity of fusion enzyme with R2 linker was increased 1.28-fold than that of native xylanase. Electrophoretic pure fusion enzyme was separated from crude enzyme extracts by affinity adsorption on corncob substrate, with 90.3% recovery in optimized conditions. Then, the fusion enzyme-corncob mixture was directly hydrolyzed. The yield of XOS reached 6.91mg/mL. The production of XOS with high purity of 91.7% was obtained by one step of centrifugation. This affinity adsorption-enzymatic reaction integrated XOS production approach provides a green route to alternative XOS traditional enzymatic production.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Chang S,Chu J,Guo Y,Li H,Wu B,He Bdoi
10.1016/j.biortech.2017.06.002subject
Has Abstractpub_date
2017-10-01 00:00:00pages
1043-1049eissn
0960-8524issn
1873-2976pii
S0960-8524(17)30889-1journal_volume
241pub_type
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