Abstract:
:To prepare fermentable hydrolysate from corncob residue (CCR), Trichoderma strain G26 was cultured on medium containing CCR for production of cellulolytic enzymes through solid-state fermentation (SSF), resulting in 71.3 IU/g (FPA), 136.2 IU/g (CMCase), 85.1 IU/g (β-glucosidase) and 11,344 IU/g (xylanase), respectively. Through a three-stage saccharification strategy, CCR was hydrolyzed by the enzymatic solution (6.5 FPU/ml) into fermentable hydrolysate containing 60.1g/l glucose (81.2% cellulose was converted at solid loading of 12.5%), 21.4% higher than that by the one-stage method. And then the hydrolysate was used to produce L-lactic acid by a previous screened strain Bacillus coagulans ZX25 in the submerged fermentation. 52.0 g/l L-lactic acid was obtained after fermentation for 44 h, with 86.5% glucose being converted to L-lactic acid. The results indicate that the strains and the hydrolysis strategy are promising for commercial production of L-lactic acid from CCR and other biomass.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Xie L,Zhao J,Wu J,Gao M,Zhao Z,Lei X,Zhao Y,Yang W,Gao X,Ma C,Liu H,Wu F,Wang X,Zhang F,Guo P,Dai Gdoi
10.1016/j.biortech.2015.06.101subject
Has Abstractpub_date
2015-10-01 00:00:00pages
331-6eissn
0960-8524issn
1873-2976pii
S0960-8524(15)00892-5journal_volume
193pub_type
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