Abstract：
:Phytase production by Aspergillus niger F3 by solid state fermentation (SSF) on citrus peel was evaluated at pilot scale under different aeration conditions. The best airflow intensity was 1 VkgM (Lair kg medium(-1) min(-1)), which allowed to produce 65 units of phytases per gram in dry basis (65 Ug(-1) d.b.) as it removed the metabolic heat generated by the microorganism, Agitation did not improve heat removal. Airflow intensity was considered as scale-up criterion. When the airflow intensity was maintained at 1 VkgM for SSF with 2 and 20 kg of medium, the kinetics parameters for biomass and enzyme concentration at the end of fermentation differed by less than 2. The air flow intensity was required to maintain the temperature and cool the SSF and to provide oxygen for microbial growth. Air flow intensity is a key a factor that must be considered when scale-up of SSF is attempted.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Rodríguez-Fernández DE,Rodríguez-León JA,de Carvalho JC,Karp SG,Sturm W,Parada JL,Soccol CRdoi
10.1016/j.biortech.2012.05.032subject
Has Abstractpub_date
2012-08-01 00:00:00pages
603-6eissn
0960-8524issn
1873-2976pii
S0960-8524(12)00776-6journal_volume
118pub_type
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