Abstract：
:Microbial generation of nocathiacin acid from nocathiacin I by manipulating enzymatic activities related to C-terminal dehydroalanine hydrolysis in Amycolatopsis fastidiosa LCB1001 was investigated by comparing the effects of various factors including metal ions, amino acid precursors and starting pH on the transformation. CuCl(2) and cysteine significantly increased bioconversion yields, and starting pH and time were also major factors affecting the product yield. Under optimal conditions, the yield of nocathiacin acid was increased from 10.4mg/L to greater than 45.0mg/L after 6days of fermentation. The use of protease inhibitors indicated that enzymes such as peptidylglycine monooxygenase and alcylase I are likely involved in the bioconversion. This is the first report on the generation of nocathiacin acid through microbial conversion in situ. Compared to chemical methods, the present approach is more effective and could be broadly applied in the production of novel derivatives of microbial secondary metabolites.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Wei M,Wang S,Fang Y,Chen Ydoi
10.1016/j.biortech.2009.12.060subject
Has Abstractpub_date
2010-05-01 00:00:00pages
3617-22issue
10eissn
0960-8524issn
1873-2976pii
S0960-8524(09)01725-8journal_volume
101pub_type
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pub_type： 杂志文章
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更新日期：2012-09-01 00:00:00
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