Abstract:
:Different culture conditions for Protaminobacter rubrum and enzymatic reaction parameters were evaluated with the goal of improving isomaltulose production. P. rubrum was grown in a medium with 1% (w/v) cane molasses and 0.5% yeast extract and achieved a maximum cell yield Y(x/s) of 0.295 g of cells/g sucrose and a specific growth rate (mu) of 0.192 h(-1). The immobilization of P. rubrum cells was carried out with calcium alginate, glutaraldehyde and polyethyleneimine. Stabile immobilized cell pellets were obtained and used 24 times in batch processes. Enzymatic conversion was carried out at different sucrose concentrations and in pH 6 medium with 70% (w/v) sucrose at 30 degrees C an isomaltulose yield of 89-94% (w/v) was obtained. The specific activity of the P. rubrum immobilized pellets in calcium alginate at 30 degrees C ranged from 1.6 to 4.0 g isomaltulose g(-1) pellet h(-1), respectively with 70% and 65% sucrose solution, while in lower sucrose concentration had higher specific activities presumably due to substrate inhibition of the isomaltulose synthase in higher sucrose concentrations.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
de Oliva-Neto P,Menão PTdoi
10.1016/j.biortech.2009.03.060subject
Has Abstractpub_date
2009-09-01 00:00:00pages
4252-6issue
18eissn
0960-8524issn
1873-2976pii
S0960-8524(09)00317-4journal_volume
100pub_type
杂志文章abstract::The binary sorption of Pb(II) and Cu(II) onto calcium alginate, algal biomass and algal/glutaraldehyde-crosslinked polyethyleneimine (PEI) composite beads was studied in the absence and presence of Ca(II). Different competitive models were compared for predicting the equilibrium data. Results show that all the sorbent...
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更新日期:2008-01-01 00:00:00
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abstract::Two sulfated zirconias were synthesized and characterized by X-ray diffraction and infrared spectroscopy. They were used as catalysts in the alcoholysis of soybean oil and in the esterification of oleic acid. Using sulfated zirconia prepared by the solvent-free method (S-ZrO(2)) as catalyst, the alcoholysis conversion...
journal_title:Bioresource technology
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doi:10.1016/j.biortech.2007.09.092
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