Isomaltulose production from sucrose by Protaminobacter rubrum immobilized in calcium alginate.

Abstract:

:Different culture conditions for Protaminobacter rubrum and enzymatic reaction parameters were evaluated with the goal of improving isomaltulose production. P. rubrum was grown in a medium with 1% (w/v) cane molasses and 0.5% yeast extract and achieved a maximum cell yield Y(x/s) of 0.295 g of cells/g sucrose and a specific growth rate (mu) of 0.192 h(-1). The immobilization of P. rubrum cells was carried out with calcium alginate, glutaraldehyde and polyethyleneimine. Stabile immobilized cell pellets were obtained and used 24 times in batch processes. Enzymatic conversion was carried out at different sucrose concentrations and in pH 6 medium with 70% (w/v) sucrose at 30 degrees C an isomaltulose yield of 89-94% (w/v) was obtained. The specific activity of the P. rubrum immobilized pellets in calcium alginate at 30 degrees C ranged from 1.6 to 4.0 g isomaltulose g(-1) pellet h(-1), respectively with 70% and 65% sucrose solution, while in lower sucrose concentration had higher specific activities presumably due to substrate inhibition of the isomaltulose synthase in higher sucrose concentrations.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

de Oliva-Neto P,Menão PT

doi

10.1016/j.biortech.2009.03.060

subject

Has Abstract

pub_date

2009-09-01 00:00:00

pages

4252-6

issue

18

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(09)00317-4

journal_volume

100

pub_type

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