Abstract：
:Rhizopus chinensis produces two lipases that catalyze ester synthesis when cultured under solid-state fermentation. The Lip2 was purified to homogeneity by ammonium sulphate precipitation, hydrophobic interaction chromatography and gel filtration chromatography. It has an apparent molecular weight of 33 kDa estimated from SDS-PAGE and 32 kDa calculated from analytical gel permeation, with synthetic activity and purification fold of 96.8 U/mg and 138.3, respectively. Maximum hydrolytic activity was obtained at pH 8.0-8.5 and 40 degrees C using pNPP as substrate. Slight activation of the enzyme was observed when Mn(2+) is present. The enzyme was most active on p-nitrophenyl laurate (C12). The purified lipase exhibited maximum synthetic activity at pH memory of 6.0 and 30 degrees C. Most of ethyl esters synthesized by lyophilized enzyme achieved good yields (>90%), and caprylic acid served as the best acyl donor. The enzyme presented a particular affinity for ethanol, n-propanol and n-hexanol, with conversion of 92%, 93% and 92%, respectively, after 20 h incubation.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Sun SY,Xu Y,Wang Ddoi
10.1016/j.biortech.2008.11.006subject
Has Abstractpub_date
2009-05-01 00:00:00pages
2607-12issue
9eissn
0960-8524issn
1873-2976pii
S0960-8524(08)00962-0journal_volume
100pub_type
杂志文章abstract：:Sugarcane press mud was pretreated by liquid hot water (LHW) at different temperatures (140-210 °C) and pre-treatment times (5-20 min) in order to assess the effects on the chemical oxygen demand (COD) solubilisation, inhibitors formation and methane yield. The experimental results showed that a high degree of biomass...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.06.107
更新日期：2014-10-01 00:00:00
abstract：:In this study, the performance of Chlorococcum sp. GD in synthetic medium with different glucose concentrations (ranging from 1 to10 g/L) was investigated. Moreover, transcriptome sequencing was conducted to clarify the response of the microalga to glucose concentrations. High concentration of glucose (6-10 g/L) not o...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.121568
更新日期：2019-09-01 00:00:00
abstract：:Studies of the land disposal of biosolids and municipal sewage have focused largely on the potential pollution of the soil with pathogens, toxic compounds or heavy metals. Little is known about the impact of sludge amendment on carbon source and sink concentrations in soils. In this study gas concentrations in Scottis...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2004.10.006
更新日期：2005-07-01 00:00:00
abstract：:The inhibitory effect of high-strength NH(3)-N on anaerobic biodegradation of landfill leachates in an EGSB bioreactor has been investigated. The research compared start-up performance of the reactor treating the landfill leachate with NH(3)-N in 242-1200 mg/l to that treating the compost leachate with NH(3)-N in 38-4...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.11.114
更新日期：2012-06-01 00:00:00
abstract：:This study proposed a novel approach of cultivating aerobic granular sludge (AGS) using intertidal wetland sediment (IWS) as inoculant in MBR for saline wastewater treatment. Granulation was observed in IWS-MBR during start-up, with increased sludge particle size (3.1-3.3 mm) and improved settling property (23.8 ml/g)...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.123715
更新日期：2020-10-01 00:00:00
abstract：:The application of periphytic biofilm in removing nitrogen from water is limited by the fluctuating nitrogen concentration. Here, we delineate a novel approach to enhance periphytic biofilm performance in nitrogen removal via upconversion luminescence of upconversion phosphors (UCPs). Nitrogen removal rates (14 d) in ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.11.079
更新日期：2019-02-01 00:00:00
abstract：:Fungi belonging to Aspergillus, Mortierella, Paecilomyces, Penicillium, Pythium, Rhizopus and Trichoderma, isolated from serpentine soil of Andaman (India) were screened for cobalt-resistance. Eleven out of total 38 isolated fungi which tolerated > 6.0 mM Co(II) were evaluated for cobalt biosorption using dried myceli...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2005.01.043
更新日期：2006-07-01 00:00:00
abstract：:A novel method was developed for fractionating cellulose microfibrils from forest residue (tulip tree sawdust) to enhance cellulose digestibility, particularly at minimum enzyme loadings. This method involved three main stages: selective hemicellulose solubilization by subcritical water (SCW) pretreatment, delignifica...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.03.069
更新日期：2013-10-01 00:00:00
abstract：:In the present study, castor oil with high free fatty acid was used for biodiesel production using heterogeneous Ni doped ZnO nanocatalyst. Ni doped ZnO nanocomposite calcinated at 800 °C has shown better catalytic activity. Process parameters on heterogeneous catalysis of castor oil into biodiesel were optimized usin...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.12.010
更新日期：2018-02-01 00:00:00
abstract：:Acidithiobacillus ferrooxidans strain SRDSM2 was isolated from silica containing soil sample collected at a Rajpardi lignite mine. The strain responded to the addition of 0.5 g/L peptone and 1.0 g/L tryptone soya broth in the ferrous sulphate tryptone soya broth (ITSB) medium with 35.3% and 29.6% increase in iron oxid...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.08.115
更新日期：2011-01-01 00:00:00
abstract：:In this study, a novel combinatorial control strategy was developed to guarantee a stable mainstream deammonification process, with three critical steps including (a) upflow airwater washing, (b) short-term increased nitrogen loading rate (NLR), and (c) low oxygen supply. Results showed that two upflow double-blanket ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.124275
更新日期：2020-12-01 00:00:00
abstract：:An anaerobic multichamber bed reactor (AMCBR) was effective in removing both molasses-chemical oxygen demand (COD), and the antibiotic oxytetracycline (OTC). The maximum COD and OTC removals were 99% in sequential AMCBR/completely stirred tank reactor (CSTR) at an OTC concentration of 300 mg L(-1). 51%, 29% and 9% of ...
journal_title：Bioresource technology
pub_type： 杂志文章,收录出版
doi：10.1016/j.biortech.2011.10.056
更新日期：2012-01-01 00:00:00
abstract：:This study investigates the application of a novel anaerobic hybrid reactor (AHR) configuration, incorporating support media for biomass immobilisation and biogas recirculation for improved mixing towards the anaerobic treatment of complex phenolic wastewater. Synthetic coal wastewater with an average phenolics and CO...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.11.035
更新日期：2013-02-01 00:00:00
abstract：:A microbial fuel cell (MFC) has great potential for treating wastewater containing azo dyes for decolourization, and simultaneous production of electricity with the help of microorganisms as biocatalysts. The concept of MFC has been already well established for the production of electricity; however, not much work has...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2012.12.063
更新日期：2013-03-01 00:00:00
abstract：:This study compared the upgrading of sugar bagasse by thermochemical and dry torrefaction methods and their corresponding combustion behavior relative to raw bagasse. The combustion reactivities were examined by non-isothermal thermogravimetric analysis. Thermochemical torrefaction was carried out by chemical pre-trea...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.10.053
更新日期：2017-01-01 00:00:00
abstract：:A pilot-scale mobile biofilter was developed where two types of wood chips (western cedar and 2 in. hardwood) were examined to treat odor emissions from a deep-pit swine finishing facility in central Iowa. The biofilters were operated continuously for 13 weeks at different air flow rates resulting in a variable empty ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2008.01.085
更新日期：2008-11-01 00:00:00
abstract：:Membrane bioreactors produce high quality effluents that could be suitable for reuse. However, when treating high strength wastewater subject to a salinity increase, a modification of biomass characteristics may occur. This circumstance is of importance, since it can have a significant impact in terms of biokinetic as...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.08.025
更新日期：2013-11-01 00:00:00
abstract：:This work investigated the effect of culture medium composition on a biosurfactant production and their total fatty acids content, as well as the surface tension of media, and biomass production by Candida ingens. A factorial experimental design was used to evaluate the combined effect of C/P, C/N(inorganic), C/Fe, C/...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2005.11.025
更新日期：2007-01-01 00:00:00
abstract：:This study aims to develop a novel technology for actual municipal wastewater treatment to achieve rapid sludge sedimentation and high pollutants removal efficiency. The SBRs were modified and operated with periodic addition of 20 μL·L-1 nanofloc®. Results revealed that NH4+-N and chemical oxygen demand (COD) was effi...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2021.124675
更新日期：2021-03-01 00:00:00
abstract：:In the present study, ethanol production from polysaccharides or wood chips was conducted in a single reactor under anaerobic conditions using the white rot fungus Schizophyllum commune NBRC 4928, which produces enzymes that degrade lignin, cellulose and hemicellulose. The ethanol yields produced from glucose and xylo...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.08.031
更新日期：2015-12-01 00:00:00
abstract：:This paper studies integrated simultaneous carbon and nitrogen removal as well as worm predation, in a circulating fluidized bed biofilm reactor (CFBBR) operated with an anoxic-aerobic bioparticle recirculation. A lab-scale CFBBR with a 8.5-liter reaction zone comprising 2L anoxic and 6.5L aerobic compartments was des...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.10.069
更新日期：2013-01-01 00:00:00
abstract：:The anaerobic co-digestion of organic fraction of municipal solid waste (OFMSW) and fruit and vegetable waste (FVW) was evaluated in terms of biogas and methane yield, volatile solids (VS) removal rate and stability of the process. The batch experiment was conducted in mesophilic conditions (35°C), with four different...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.01.003
更新日期：2017-03-01 00:00:00
abstract：:In situ cell separation and immobilization of bacterial cells for biodesulfurization were developed by using superparamagnetic Fe(3)O(4) nanoparticles (NPs). The Fe(3)O(4) NPs were synthesized by coprecipitation followed by modification with ammonium oleate. The surface-modified NPs were monodispersed and the particle...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.05.064
更新日期：2009-11-01 00:00:00
abstract：:Impact of gas diffusion electrodes (GDEs) was evaluated in enhancing the CO2 bio-availability for its transformation to C4-organics, especially to alcohols using selective mixed culture. Observed current density was more stable (9-11 A/m2) than submerged experiments reported and significantly varied with pH and respec...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.02.058
更新日期：2018-10-01 00:00:00
abstract：:Continuous fixed bed reactors filled by three kinds of packing which were glass bead, glass spring and Dixon rings were investigated. The effect of temperature, pressure, the molar ratio of methanol to oil, flow rate, the size and shape of the packing were researched. The highest yield 90.84% of FAME was obtained by f...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.12.085
更新日期：2017-03-01 00:00:00
abstract：:To improve the production of glucose from cellulose, a simple and effective route was developed. This process uses a combination of a step of cellulose dissolution in aqueous NaOH/urea solution and then regeneration with water, followed by an acid hydrolysis step under microwave irradiation. The method is effective to...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.05.045
更新日期：2015-09-01 00:00:00
abstract：:After extensive analysis, Ulva lactuca dried algae, collected from the Monastir coastal zone, was proven to be successful as an adsorbent for the removal of certain inorganic pollutants. The main objective of this study was the nonlinear modeling of heavy metal removal from an aqueous solution, using a freely availabl...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.08.107
更新日期：2011-01-01 00:00:00
abstract：:In this work, multiple reuses of Rhodococcus ruber TH3 free cells for the hydration of acrylonitrile to produce acrylamide in a membrane dispersion microreactor were carried out. Through using a centrifuge, the reactions reached 39.9, 39.5, 38.6 and 38.0wt% of the final acrylamide product concentration respectively wi...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.03.134
更新日期：2015-01-01 00:00:00
abstract：:Fe is widely used as an additive in anaerobic digestion, but its bioavailability and the mechanism by which it enhances digestion are unclear. In this study, sequential extraction was used to measure Fe bioavailability, while biochemical parameters, kinetics model and Q-PCR (fluorescence quantitative PCR) were used to...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.07.151
更新日期：2018-02-01 00:00:00
abstract：:A kinetic model is devised, from the reaction mechanism steps, to predict the rate of reducing sugar production by hydrolysis of two types of cellulose, namely, amorphous carboxymethylcellulose (CMC) and highly crystalline wood shavings, using Aspergillus niger cellulase. Experimental results in a stirred batch reacto...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.09.003
更新日期：2008-07-01 00:00:00
