Novel minor lipase from Rhizopus chinensis during solid-state fermentation: biochemical characterization and its esterification potential for ester synthesis.


:Rhizopus chinensis produces two lipases that catalyze ester synthesis when cultured under solid-state fermentation. The Lip2 was purified to homogeneity by ammonium sulphate precipitation, hydrophobic interaction chromatography and gel filtration chromatography. It has an apparent molecular weight of 33 kDa estimated from SDS-PAGE and 32 kDa calculated from analytical gel permeation, with synthetic activity and purification fold of 96.8 U/mg and 138.3, respectively. Maximum hydrolytic activity was obtained at pH 8.0-8.5 and 40 degrees C using pNPP as substrate. Slight activation of the enzyme was observed when Mn(2+) is present. The enzyme was most active on p-nitrophenyl laurate (C12). The purified lipase exhibited maximum synthetic activity at pH memory of 6.0 and 30 degrees C. Most of ethyl esters synthesized by lyophilized enzyme achieved good yields (>90%), and caprylic acid served as the best acyl donor. The enzyme presented a particular affinity for ethanol, n-propanol and n-hexanol, with conversion of 92%, 93% and 92%, respectively, after 20 h incubation.


Bioresour Technol


Bioresource technology


Sun SY,Xu Y,Wang D




Has Abstract


2009-05-01 00:00:00














  • Microbial oil produced from biodiesel by-products could enhance overall production.

    abstract::Glycerol and rapeseed meal, two major by-products of biodiesel production, have been tested for possible use as low-cost raw materials for the production of microbial bio-oil using the oleaginous yeast Rhodosporidium toruloides. Using fed-batch fermentation with crude glycerol and a novel nitrogen rich nutrient source...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Uçkun Kiran E,Trzcinski A,Webb C

    更新日期:2013-02-01 00:00:00

  • Sporulation and spore stability of Bacillus megaterium enhance Ketogulonigenium vulgare propagation and 2-keto-L-gulonic acid biosynthesis.

    abstract::Bacillus spp. is widely used as the companion bacterium in the two-step biosynthesis of 2-keto-L-gulonic acid (2-KLG), which is the direct precursor in the production of vitamin C by Ketogulonigenium vulgare. To understand the effects of sporulation and spore stability on 2-KLG production, the spo0A and spoVFA deletio...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Zhu Y,Liu J,Du G,Zhou J,Chen J

    更新日期:2012-03-01 00:00:00

  • Isolation and characterization of bacterium producing lipid from short-chain fatty acids.

    abstract::Anaerobic fermentation generates propionic acid, which inhibits microbial growth and accumulates in wastewater containing increased amounts of organic matter. We therefore isolated a propionic acid-assimilating bacterium that could produce triacylglycerol, for use in wastewater treatment. Nitratireductor sp. strain OM...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Okamura Y,Nakai S,Ohkawachi M,Suemitsu M,Takahashi H,Aki T,Matsumura Y,Tajima T,Nakashimada Y,Matsumoto M

    更新日期:2016-02-01 00:00:00

  • Comprehensive modeling and investigation of the effect of iron on the growth rate and lipid accumulation of Chlorella vulgaris cultured in batch photobioreactors.

    abstract::Recent works have shown that specific strains of microalgae are capable to simultaneously increase their growth rate and lipid content when cultured under suitable concentrations of iron. While these results are promising in view of the exploitation of microalgae for producing biofuels, to the best of our knowledge, n...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Concas A,Steriti A,Pisu M,Cao G

    更新日期:2014-02-01 00:00:00

  • Alkali/urea pretreatment of rice straw at low temperature for enhanced biological hydrogen production.

    abstract::A pretreatment process using alkali/urea solution at low temperature was proposed for enhanced cellulosic biohydrogen production. Different alkaline solutions with both presence and absence of urea were studied. It can be found NaOH/Urea pretreatment exhibited excellent pretreatment performance at temperature from -8 ...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Dong L,Cao G,Zhao L,Liu B,Ren N

    更新日期:2018-11-01 00:00:00

  • Co-gasification of tire and biomass for enhancement of tire-char reactivity in CO2 gasification process.

    abstract::In this investigation, palm empty fruit bunch (EFB) and almond shell (AS) were implemented as two natural catalysts rich in alkali metals, especially potassium, to enhance the reactivity of tire-char through co-gasification process. Co-gasification experiments were conducted at several blending ratios using isothermal...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Lahijani P,Zainal ZA,Mohamed AR,Mohammadi M

    更新日期:2013-06-01 00:00:00

  • Nutrient changes and biodynamics of epigeic earthworm Perionyx excavatus (Perrier) during recycling of some agriculture wastes.

    abstract::Potential of an oriental composting earthworm: Perionyx excavatus (Perrier) to decompose waste resources generated from agricultural practices (crop residues, farm yard manure, and cattle dung) was studied for 150days under laboratory conditions. At the end of experiment, all vermibeds showed significant decrease in t...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Suthar S

    更新日期:2007-05-01 00:00:00

  • Effects of temperature, oxygen and steam on pore structure characteristics of coconut husk activated carbon powders prepared by one-step rapid pyrolysis activation process.

    abstract::Activated carbon powders made from coconut husk (CHCs) were prepared by one-step rapid pyrolysis activation process. Effects of temperature, oxygen and steam on the pore structure of CHCs were investigated. Results showed that high temperature, oxygen and steam all motivated the development of the CHCs pore structure....

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Fu J,Zhang J,Jin C,Wang Z,Wang T,Cheng X,Ma C

    更新日期:2020-08-01 00:00:00

  • Oil extraction from Scenedesmus obliquus using a continuous microwave system--design, optimization, and quality characterization.

    abstract::A 1.2 kW, 2450 MHz resonant continuous microwave processing system was designed and optimized for oil extraction from green algae (Scenedesmus obliquus). Algae-water suspension (1:1 w/w) was heated to 80 and 95°C, and subjected to extraction for up to 30 min. Maximum oil yield was achieved at 95°C and 30 min. The micr...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Balasubramanian S,Allen JD,Kanitkar A,Boldor D

    更新日期:2011-02-01 00:00:00

  • High quality fuel gas from biomass pyrolysis with calcium oxide.

    abstract::The removal of CO2 and tar in fuel gas produced by biomass thermal conversion has aroused more attention due to their adverse effects on the subsequent fuel gas application. High quality fuel gas production from sawdust pyrolysis with CaO was studied in this paper. The results of pyrolysis-gas chromatography/mass spec...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Zhao B,Zhang X,Chen L,Sun L,Si H,Chen G

    更新日期:2014-03-01 00:00:00

  • Effects of substrate moisture content, log weight and filter porosity on shiitake (Lentinula edodes) yield.

    abstract::Production costs for shiitake (Lentinula edodes) are on the rise in the United States due to increasing expenses including materials, labor and energy. Increased yield and improved bioconversion of raw materials may improve grower profit margins and may help reduce the cost of shiitake to the consumer. Two crops (Crop...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Shen Q,Liu P,Wang X,Royse DJ

    更新日期:2008-11-01 00:00:00

  • Role of NH3 recycling on nitrogen fractions during sludge composting.

    abstract::The aim of this study is to reduce nitrogen (N) loss and investigate the role of ammonia (NH3) recycling on N fractions, environmental factors and bacterial communities. In this study, collected NH3 from composting and recycled in it. The results showed that NH3 recycling affected N-cycling processes such as nitrifica...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Wang R,Zhao Y,Xie X,Mohamed TA,Zhu L,Tang Y,Chen Y,Wei Z

    更新日期:2020-01-01 00:00:00

  • Effect of phenol addition on COD and nitrate removal in an anoxic suspension reactor.

    abstract::In this study, a denitrifying culture was enriched in a continuously re-circulated anoxic suspension reactor fed with glucose and nitrate for about 8 months (stage I) under different organic loading rates (OLR). At the end of stage I, the removal efficiency for NO(3)(-)-N was 80% with 93% COD (5 g/l) removal at an OLR...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Bajaj M,Gallert C,Winter J

    更新日期:2010-07-01 00:00:00

  • Production of oligosaccharides and biofuels from Miscanthus using combinatorial steam explosion and ionic liquid pretreatment.

    abstract::Pretreatment strategies are fundamental to effectively deconstruct lignocellulosic biomass and economically produce biofuels, biomaterials and bio-based chemicals. This study evaluated individual and combinatorial steam explosion (SE) and ionic liquid (IL) pretreatments for production of high-value oligosaccharides fr...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Bhatia R,Lad JB,Bosch M,Bryant DN,Leak D,Hallett JP,Franco TT,Gallagher JA

    更新日期:2020-12-28 00:00:00

  • Semi-continuous biohydrogen production as an approach to generate electricity.

    abstract::In this work, a semi-continuous biological system was established to produce hydrogen and generate electricity by coupling the bioreactor to a fuel cell. Heat and acid pretreatments (at 35 and 55 degrees C) of a seed sludge used as inoculum were performed in order to increase hydrogen producers. Different initial gluc...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: García-Peña EI,Guerrero-Barajas C,Ramirez D,Arriaga-Hurtado LG

    更新日期:2009-12-01 00:00:00

  • The comparison of the migration and transformation behavior of heavy metals during pyrolysis and liquefaction of municipal sewage sludge, paper mill sludge, and slaughterhouse sludge.

    abstract::Municipal sewage sludge, paper mill sludge, and slaughterhouse sludge were pyrolyzed and liquefied for the production of bio-char. The migration and transformation behavior of Cu, Cr, and Zn during pyrolysis and liquefaction of these sludges were studied. Pyrolysis and liquefaction promoted mobile fraction (F1 and F2)...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Shao J,Yuan X,Leng L,Huang H,Jiang L,Wang H,Chen X,Zeng G

    更新日期:2015-12-01 00:00:00

  • Understanding the impact of influent nitrogen concentration on granule size and microbial community in a granule-based enhanced biological phosphorus removal system.

    abstract::To better understand the effect of influent nitrogen concentration on granule size and microbial community in a granule-based enhanced biological phosphorus removal system, three influent nitrogen concentrations were tested while carbon concentration was an unlimited factor. The results show that although ammonium and...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Zou J,Li Y,Zhang L,Wang R,Sun J

    更新日期:2015-02-01 00:00:00

  • Modelling the biodegradation of non-steroidal anti-inflammatory drugs (NSAIDs) by activated sludge and a pure culture.

    abstract::A model describing ibuprofen and ketoprofen biodegradation by activated sludge from three different wastewater treatment plants (WWTP) was developed in this study. This model successfully described the biodegradation profiles observed at two different initial concentrations of each compound, where a lag-phase was obse...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Almeida B,Oehmen A,Marques R,Brito D,Carvalho G,Crespo MT

    更新日期:2013-04-01 00:00:00

  • Use of rice husk for the adsorption of congo red from aqueous solution in column mode.

    abstract::A continuous fixed bed study was carried out by using rice husk as a biosorbent for the removal of congo red (CR) from aqueous solution. The effects of important factors, such as the value of initial pH, existing salt, the flow rate, the influent concentration of CR and bed depth, were studied. Data confirmed that the...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Han R,Ding D,Xu Y,Zou W,Wang Y,Li Y,Zou L

    更新日期:2008-05-01 00:00:00

  • Bioleaching of rare earth and radioactive elements from red mud using Penicillium tricolor RM-10.

    abstract::The aim of this work is to investigate biological leaching of rare earth elements (REEs) and radioactive elements from red mud, and to evaluate the radioactivity of the bioleached red mud used for construction materials. A filamentous, acid-producing fungi named RM-10, identified as Penicillium tricolor, is isolated f...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Qu Y,Lian B

    更新日期:2013-05-01 00:00:00

  • Catalytic pyrolysis of sugarcane bagasse by using microwave heating.

    abstract::The aim of this study was to research the catalytic effects on the microwave pyrolysis of sugarcane bagasse and thus to discuss the reaction performance, product distribution, and kinetic analysis. With the addition of metal-oxides served as catalysts, reaction results such as mass reduction ratio and reaction rate in...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Kuan WH,Huang YF,Chang CC,Lo SL

    更新日期:2013-10-01 00:00:00

  • Decisive role of vacuum-assisted carbonization in valorization of lignin-enriched (Juglans regia-shell) biowaste.

    abstract::Bioenergy is considered a sustainable substitute to fossil-fuel resources and the development of a prudent combination of renewable and innovative conversion technologies are essential for the valorization and effective conversion of biowaste to value-added commodities. Here, a negative pressure-induced carbonization ...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Yousaf B,Liu G,Ubaid Ali M,Abbas Q,Liu Y,Ullah H,Imtiyaz Cheema A

    更新日期:2020-12-14 00:00:00

  • Effect of light conditions on mixotrophic cultivation of green microalgae.

    abstract::Current research aimed to increase mixotrophic biomass from various organic carbon sources by exploring best light conditions. Three substrates glucose, acetic acid and glycerol were studied for their effects on mixotrophic microalgae cultivation under four light conditions. Light irradiance exhibited variability in g...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Patel AK,Joun JM,Hong ME,Sim SJ

    更新日期:2019-06-01 00:00:00

  • The distribution of Pb(II)/Cd(II) adsorption mechanisms on biochars from aqueous solution: Considering the increased oxygen functional groups by HCl treatment.

    abstract::The adsorption mechanisms of Pb(II) and Cd(II) in aqueous solution using camellia seed husk biochars pyrolyzed at different temperatures were studied. The adsorption of Pb(II) and Cd(II) on biochars are mainly controlled by ion exchange, oxygen functional groups (OFGs) complexation, Pb(II)/Cd(II)-π interactions, and p...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Wu J,Wang T,Zhang Y,Pan WP

    更新日期:2019-11-01 00:00:00

  • Nitritation-denitritation in landfill leachate with glycerine as a carbon source.

    abstract::The effects of limited oxygen concentration (0.7 mg O2/L) in the aeration phase of the SBR cycle and glycerine as an additional carbon source on the effectiveness of nitritation-denitritation and sludge production during municipal landfill leachate treatment were examined. As carbon sources, sodium acetate (Ac) and so...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Kulikowska D,Bernat K

    更新日期:2013-08-01 00:00:00

  • Denitrification performance and mechanism of a novel isolated Acinetobacter sp. FYF8 in oligotrophic ecosystem.

    abstract::The main purpose of this study is to isolate and purify oligotrophic denitrifying bacteria, Acinetobacter sp. FYF8, so as to study the denitrification capacity and characteristics in response to oligotrophic ecosystem. The RSM showed that the best denitrification efficiency was 97.90% under 7.58 pH, 20.69 °C temperatu...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Fan Y,Su J,Zheng Z,Gao J,Ali A

    更新日期:2021-01-01 00:00:00

  • Characteristics of wastewater and mixed liquor and their role in membrane fouling.

    abstract::Effects of wastewater and mixed liquor characteristics on membrane fouling in both a submerged anaerobic membrane bioreactor and a thermophilic submerged aerobic membrane bioreactor were studied with four types of industrial wastewaters. Significant differences in particle size distribution, colloidal content, the pro...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Gao WJ,Han MN,Qu X,Xu C,Liao BQ

    更新日期:2013-01-01 00:00:00

  • Long-term effect of set potential on biocathodes in microbial fuel cells: electrochemical and phylogenetic characterization.

    abstract::The long-term effect of set potential on oxygen reducing biocathodes was investigated in terms of electrochemical and biological characteristics. Three biocathodes were poised at 200, 60 and -100 mV vs. saturated calomel electrode (SCE) for 110 days, including the first 17 days for startup. Electrochemical analyses sh...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Xia X,Sun Y,Liang P,Huang X

    更新日期:2012-09-01 00:00:00

  • Microbial community acclimatization for enhancement in the methane productivity of anaerobic co-digestion of fats, oil, and grease.

    abstract::The methane productivity and long chain fatty acids (LCFAs) degradation capability of unacclimatized seed sludge (USS) and acclimatized seed sludge (ASS) at different substrate ratios of fats oil and grease (FOG) and mixed sewage sludge were investigated in this study. Biogas produced in ASS in initial phase of anaero...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Kurade MB,Saha S,Kim JR,Roh HS,Jeon BH

    更新日期:2020-01-01 00:00:00

  • Enhanced isophthalonitrile complexation-reduction removal using a novel anaerobic fluidized bed reactor in a bioelectrochemical system based on electric field activation (AFBR-EFA).

    abstract::On account of the recalcitrant and highly toxicity of organonitrile substrates, traditional processes are limited by HCN poisoning thus inefficient. This article proposed a novel anaerobic fluidized bed reactor with electric field activation (AFBR-EFA) which had a 260-day continuous operation. The operation aims to ex...

    journal_title:Bioresource technology

    pub_type: 杂志文章


    authors: Cui T,Wang Y,Wang X,Zhang Y,Han W,Li J,Sun X,Shen J,Wang L

    更新日期:2020-03-03 00:00:00