当前位置： SCI文献检索 > BIORESOURCE TECHNOLOGY期刊下所有文献 > Novel minor lipase from Rhizopus chinensis during solid-state fermentation: biochemical characterization and its esterification potential for ester synthesis.

Novel minor lipase from Rhizopus chinensis during solid-state fermentation: biochemical characterization and its esterification potential for ester synthesis.

Abstract：

:Rhizopus chinensis produces two lipases that catalyze ester synthesis when cultured under solid-state fermentation. The Lip2 was purified to homogeneity by ammonium sulphate precipitation, hydrophobic interaction chromatography and gel filtration chromatography. It has an apparent molecular weight of 33 kDa estimated from SDS-PAGE and 32 kDa calculated from analytical gel permeation, with synthetic activity and purification fold of 96.8 U/mg and 138.3, respectively. Maximum hydrolytic activity was obtained at pH 8.0-8.5 and 40 degrees C using pNPP as substrate. Slight activation of the enzyme was observed when Mn(2+) is present. The enzyme was most active on p-nitrophenyl laurate (C12). The purified lipase exhibited maximum synthetic activity at pH memory of 6.0 and 30 degrees C. Most of ethyl esters synthesized by lyophilized enzyme achieved good yields (>90%), and caprylic acid served as the best acyl donor. The enzyme presented a particular affinity for ethanol, n-propanol and n-hexanol, with conversion of 92%, 93% and 92%, respectively, after 20 h incubation.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Sun SY,Xu Y,Wang D

doi

10.1016/j.biortech.2008.11.006

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

2607-12

issue

9

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(08)00962-0

journal_volume

100

pub_type

杂志文章

相关文献

BIORESOURCE TECHNOLOGY文献大全
  • Nitrogen or phosphorus repletion strategies for enhancing lipid or carotenoid production from Tetraselmis marina.

    abstract：:The objective of this study was to investigate the accumulation of lipid and photosynthetic pigments from Tetraselmis marina. When the cells were grown in F/2-medium for seven days in the first stage, the carotenoid and lipid contents, and productivity were 44g/kg (DW), 27% and 31mg/L/d, respectively. After second sta...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2017.04.008

    authors： Dahmen-Ben Moussa I,Chtourou H,Karray F,Sayadi S,Dhouib A

    更新日期：2017-08-01 00:00:00

  • Multiple reuses of Rhodococcus ruber TH3 free cells to produce acrylamide in a membrane dispersion microreactor.

    abstract：:In this work, multiple reuses of Rhodococcus ruber TH3 free cells for the hydration of acrylonitrile to produce acrylamide in a membrane dispersion microreactor were carried out. Through using a centrifuge, the reactions reached 39.9, 39.5, 38.6 and 38.0wt% of the final acrylamide product concentration respectively wi...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2015.03.134

    authors： Li J,Liu J,Chen J,Wang Y,Luo G,Yu H

    更新日期：2015-01-01 00:00:00

  • Biodiesel production in a membrane reactor using MCM-41 supported solid acid catalyst.

    abstract：:Production of biodiesel from the transesterification between soybean oil and methanol was conducted in this study by a membrane reactor, in which ceramic membrane was packed with MCM-41 supported p-toluenesulfonic acid (PTSA). Box-Behnken design and response surface methodology (RSM) were used to investigate the effec...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2014.03.004

    authors： Xu W,Gao L,Wang S,Xiao G

    更新日期：2014-05-01 00:00:00

  • Modification of paper properties by the pretreatment of wastepaper pulp with Graphiumputredinis, Trichodermaharzianum and fusant xylanases.

    abstract：:Graphiumputredinis, Trichodermaharzianum and fusant were used in the present study to produce extracellular xylanases, an important industrial enzyme used in pulp and paper industry produced in a minimal medium supplemented with oat spelt xylan (1%, w/v) pH 7.0 at 27+/-2 degrees C. The enzyme was purified to homogenei...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2008.07.014

    authors： Savitha S,Sadhasivam S,Swaminathan K

    更新日期：2009-01-01 00:00:00

  • Impact of wet aerobic pretreatments on cellulose accessibility and bacterial communities in rape straw.

    abstract：:A new pretreatment method of lignocellulosic biomass was explored by using a wet aerobic process with an alkaline lignin and a mineral salt solution. This treatment significantly improved structural modification of rape straw used as substrate model in this study. Change in cellulose accessibility to cellulase of rape...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2017.03.142

    authors： Tian JH,Pourcher AM,Bize A,Wazeri A,Peu P

    更新日期：2017-08-01 00:00:00

  • Studies on the production of enantioselective nitrilase in a stirred tank bioreactor by Pseudomonas putida MTCC 5110.

    abstract：:Nitrilases constitute an important class of hydrolases, having numerous industrial applications. The present work aims to address the production of nitrile hydrolyzing enzymes from Pseudomonas putida MTCC 5110 in a 6l bioreactor. Effect of various physico-chemical conditions and process parameters like pH, temperature...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2006.11.053

    authors： Naik SC,Kaul P,Barse B,Banerjee A,Banerjee UC

    更新日期：2008-01-01 00:00:00

  • Simulation analysis and ternary diagram of municipal solid waste pyrolysis and gasification based on the equilibrium model.

    abstract：:A self-sustained municipal solid waste (MSW) pyrolysis-gasification process with self-produced syngas as heat source was proposed and an equilibrium model was established to predict the syngas reuse rate considering variable MSW components. Simulation results indicated that for constant moisture (ash) content, with th...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2017.03.072

    authors： Deng N,Zhang A,Zhang Q,He G,Cui W,Chen G,Song C

    更新日期：2017-07-01 00:00:00

  • Plasmid as a measure of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid.

    abstract：:The purpose of this research was to pursuit the quantification of microbial degradation capacity for 2,4-dichlorophenoxyacetic acid (2,4-D) by detecting and quantifying a prominent 2,4-D degradation encoding plasmid. Batch reactor acclimation, de-acclimation, and re-acclimation tests were conducted during which period...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2008.09.016

    authors： Chong NM,Chang HW

    更新日期：2009-02-01 00:00:00

  • Enhanced Coulombic efficiency in glucose-fed microbial fuel cells by reducing metabolite electron losses using dual-anode electrodes.

    abstract：:Glucose-fed microbial fuel cells (MFCs) have displayed low Coulombic efficiency (CE); one reason for a low CE is metabolite generation, causing significant electron loss within MFC systems. In the present study, notable electron loss (15.83%) is observed in glucose-fed MFCs due to residual propionate, a glucose metabo...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2010.12.036

    authors： Kim KY,Chae KJ,Choi MJ,Ajayi FF,Jang A,Kim CW,Kim IS

    更新日期：2011-03-01 00:00:00

  • Determination of aeration rate and kinetics of composting some agricultural wastes.

    abstract：:This study aimed to determine the aeration rate and its kinetics in aerobic composting of agricultural wastes. For this aim compost materials were prepared by mixing grass trimmings, tomato, pepper, and eggplant wastes. Four vertical forced aeration type reactors and one vertical natural convection type reactor were m...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2003.10.007

    authors： Kulcu R,Yaldiz O

    更新日期：2004-05-01 00:00:00

  • Effect of microwave irradiated Methanosarcina barkeri DSM-804 on biomethanation.

    abstract：:Methanosarcina barkeri DSM-804, a methanogenic bacterium, when exposed to microwave radiation of frequencies ranging from 13.5 to 36.5 GHz, showed faster growth in comparison to the unirradiated bacterial culture. Methane concentration in the biogas generated from the irradiated culture was higher than that from unirr...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2005.04.032

    authors： Banik S,Bandyopadhyay S,Ganguly S,Dan D

    更新日期：2006-04-01 00:00:00

  • Advanced nitrogen removal from wastewater by combining anammox with partial denitrification.

    abstract：:The anammox (anaerobic ammonium oxidation) process has attracted much attention for its cost-saving. However, excess nitrate is usually produced which should be further treated. In this study, an innovative process combined anammox with partial denitrification (nitrate→nitrite) was proposed for advanced nitrogen remov...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2014.12.043

    authors： Du R,Peng Y,Cao S,Wang S,Wu C

    更新日期：2015-03-01 00:00:00

  • Role of macrophyte and effect of supplementary aeration in up-flow constructed wetland-microbial fuel cell for simultaneous wastewater treatment and energy recovery.

    abstract：:This study investigates the role of plant (Elodea nuttallii) and effect of supplementary aeration on wastewater treatment and bioelectricity generation in an up-flow constructed wetland-microbial fuel cell (UFCW-MFC). Aeration rates were varied from 1900 to 0mL/min and a control reactor was operated without supplement...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2016.10.079

    authors： Oon YL,Ong SA,Ho LN,Wong YS,Dahalan FA,Oon YS,Lehl HK,Thung WE,Nordin N

    更新日期：2017-01-01 00:00:00

  • Xylo-oligosaccharides are competitive inhibitors of cellobiohydrolase I from Thermoascus aurantiacus.

    abstract：:The effects of xylo-oligosaccharides (XOS) and xylose on the hydrolytic activities of cellulases, endoglucanase II (EGII, originating from Thermoascus aurantiacus), cellobiohydrolase I (CBHI, from T. aurantiacus), and cellobiohydrolase II (CBHII, from Trichoderma reesei) on Avicel and nanocellulose were investigated. ...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2012.04.072

    authors： Zhang J,Viikari L

    更新日期：2012-08-01 00:00:00

  • Bioleaching of chalcopyrite by defined mixed moderately thermophilic consortium including a marine acidophilic halotolerant bacterium.

    abstract：:A defined mixed moderately thermophilic consortium including three terrestrial microorganisms (Leptospirillum ferriphilum, Acidithiobacillus caldus and Ferroplasma thermophilum) and a marine acidophilic halotolerant bacterium (Sulfobacillus sp. TPY) was constructed to evaluate its ability for bioleaching of chalcopyri...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2012.06.114

    authors： Wang Y,Su L,Zhang L,Zeng W,Wu J,Wan L,Qiu G,Chen X,Zhou H

    更新日期：2012-10-01 00:00:00

  • Lignin materials for adsorption: Current trend, perspectives and opportunities.

    abstract：:Lignin is a highly aromatic low value biomass residue, which can be utilized for chemicals, fuels and materials production. In recent years significant attention has focused on adsorbent materials from lignin. However, only 5% of available lignin is exploited worldwide, thus significant opportunities still exist for m...

    journal_title：Bioresource technology

    pub_type： 杂志文章,评审

    doi：10.1016/j.biortech.2018.09.139

    authors： Supanchaiyamat N,Jetsrisuparb K,Knijnenburg JTN,Tsang DCW,Hunt AJ

    更新日期：2019-01-01 00:00:00

  • Comparison of kinetic analysis methods in thermal decomposition of cattle manure by themogravimetric analysis.

    abstract：:The thermogravimetric (TG) experiments of cattle manure were carried out from room temperature to 900°C at five different heating rates (10, 20, 30, 40 and 50°C/min) and the kinetics of the main decomposition process were analyzed with different methods. TG curves indicate that the major decomposition process of cattl...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2017.06.007

    authors： Chen G,He S,Cheng Z,Guan Y,Yan B,Ma W,Leung DYC

    更新日期：2017-11-01 00:00:00

  • Gasification of torrefied Miscanthus × giganteus in an air-blown bubbling fluidized bed gasifier.

    abstract：:Torrefaction is suggested to be an effective method to improve the fuel properties of biomass and gasification of torrefied biomass should provide a higher quality product gas than that from unprocessed biomass. In this study, both raw and torrefied Miscanthus × giganteus (M×G) were gasified in an air-blown bubbling f...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2014.02.094

    authors： Xue G,Kwapinska M,Horvat A,Kwapinski W,Rabou LP,Dooley S,Czajka KM,Leahy JJ

    更新日期：2014-05-01 00:00:00

  • Dominant thermogravimetric signatures of lignin in cashew shell as compared to cashew shell cake.

    abstract：:Dominant thermogravimetric signatures related to lignin were observed in cashew shell as compared to these signatures in cashew shell cake. The phenomenon of weakening of lignin from cashew shell to cashew shell cake was explained on the basis of changes in the activation energies. The pertinent temperature regimes re...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2013.12.087

    authors： Gangil S

    更新日期：2014-03-01 00:00:00

  • Cost-effective biodiesel production from wet microalgal biomass by a novel two-step enzymatic process.

    abstract：:In this study, a novel two-step enzymatic process was firstly established to produce microalgae biodiesel using wet Chlorella biomass. In the first hydrolysis step, to reduce energy consumption and effectively disrupt microalgal cell wall, among cellulase, hemicellulase, papain, lysozyme and pectinase, the highest hyd...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2018.08.038

    authors： He Y,Wu T,Wang X,Chen B,Chen F

    更新日期：2018-11-01 00:00:00

  • Carbon and nutrient removal from centrates and domestic wastewater using algal-bacterial biofilm bioreactors.

    abstract：:The mechanisms of carbon and nutrient removal in an open algal-bacterial biofilm reactor and an open bacterial biofilm reactor were comparatively evaluated during the treatment of centrates and domestic wastewater. Comparable carbon removals (>80%) were recorded in both bioreactors, despite the algal-bacterial biofilm...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2013.04.008

    authors： Posadas E,García-Encina PA,Soltau A,Domínguez A,Díaz I,Muñoz R

    更新日期：2013-07-01 00:00:00

  • Bioleaching of copper sulfides using mixed microorganisms and its community structure succession in the presence of seawater.

    abstract：:The bacterial diversity and dynamics in the leaching solution were analyzed during bioleaching of low-grade copper sulfide ore in the presence of seawater in this study. The results indicated a promoting response of appropriate-proportion seawater to bioleaching with improved copper recoveries. A maximum of 84.70% cop...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2019.122453

    authors： Chen W,Yin S,Wu A,Wang L,Chen X

    更新日期：2020-02-01 00:00:00

  • A techno-economic evaluation of anaerobic biogas producing systems in developing countries.

    abstract：:Biogas production has been the focus of many individuals in the developing world; there have been several investigations that focus on improving the production process and product quality. In the developing world the lack of advanced technology and capital has hindered the development of energy production. Renewable e...

    journal_title：Bioresource technology

    pub_type： 杂志文章,评审

    doi：10.1016/j.biortech.2017.12.013

    authors： Morgan HM Jr,Xie W,Liang J,Mao H,Lei H,Ruan R,Bu Q

    更新日期：2018-02-01 00:00:00

  • Partial nitrification and denitrification of mature landfill leachate using a pilot-scale continuous activated sludge process at low dissolved oxygen.

    abstract：:Controlling of low dissolved oxygen (DO) levels (0.1-0.5mg/L), a cost-effective strategy, was applied to a pilot-scale anoxic-oxic-oxic-anoxic process for partial nitrification and denitrification of mature landfill leachate. High ammonium removal efficiency, stable nitrite accumulation rate and total nitrogen removal...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2016.07.008

    authors： Chen Z,Wang X,Yang Y,Mirino MW Jr,Yuan Y

    更新日期：2016-10-01 00:00:00

  • Purification and biochemical characterization of extracellular laccase from the ascomycete Paraconiothyrium variabile.

    abstract：:An extracellular laccase-producing ascomycete was isolated from soil and identified as Paraconiothyrium variabile using rDNA sequence analysis. Typical laccase substrates including 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS), 2,6-dimethoxyphenol (DMP), and guaiacol were oxidized by the purified enzyme (d...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2010.09.043

    authors： Forootanfar H,Faramarzi MA,Shahverdi AR,Yazdi MT

    更新日期：2011-01-01 00:00:00

  • A novel two-phase bioprocess for the production of Arthrospira (Spirulina) maxima LJGR1 at pilot plant scale during different seasons and for phycocyanin induction under controlled conditions.

    abstract：:A two-phase outdoor cultivation bioprocess for Arthrospira maxima LJGR1 combined with phycocyanin induction in concentrated cultures under controlled conditions was evaluated using a modified low-cost Zarrouk medium. Growth was monitored during 4 cycles in 2018 and 4 cycles in 2019. Biomass was harvested and concentra...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2019.122548

    authors： García-López DA,Olguín EJ,González-Portela RE,Sánchez-Galván G,De Philippis R,Lovitt RW,Llewellyn CA,Fuentes-Grünewald C,Parra Saldívar R

    更新日期：2020-02-01 00:00:00

  • A comparative account of glucose yields and bioethanol production from separate and simultaneous saccharification and fermentation processes at high solids loading with variable PEG concentration.

    abstract：:A process strategy to aid in optimal enzymatic hydrolysis through the addition of polyethylene glycol (PEG6000) was tested for separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF). Pretreated wheat straw at 30% solids (w/w) loading was enzymatically hydrolyzed with 0, 0.5...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2019.03.060

    authors： Kadhum HJ,Mahapatra DM,Murthy GS

    更新日期：2019-07-01 00:00:00

  • Effect of pH on growth and lipid accumulation kinetics of the microalga Chlorella vulgaris grown heterotrophically under sulfur limitation.

    abstract：:The goal of the present study was to investigate the pH range that can support the growth of C. vulgaris, and, more specifically, to identify the optimal pH for the microalga's growth, under heterotrophic conditions. Furthermore, the effect of pH on the accumulation of intracellular lipids was studied. A wide range of...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2016.08.033

    authors： Sakarika M,Kornaros M

    更新日期：2016-11-01 00:00:00

  • Influence of alcohol treatments on the activity of lipases immobilized on methyl-modified silica aerogels.

    abstract：:The effects of alcohol treatment on the activity and loading amount of Candida rugosa lipase (CRL), Candida Antarctica lipase B (CALB) and Porcine Pancreas lipase (PPL) immobilized on methyl-modified silica aerogels were investigated, and the fluorescent analysis was used to explore the change of lipase hydrophobicity...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2010.04.089

    authors： Gao S,Wang W,Wang Y,Luo G,Dai Y

    更新日期：2010-10-01 00:00:00

  • Comparison of sodium hydroxide and calcium hydroxide pretreatments on the enzymatic hydrolysis and lignin recovery of sugarcane bagasse.

    abstract：:Sodium hydroxide (NaOH) and calcium hydroxide (Ca(OH)2) respectively dissolved in water and 70% glycerol were applied to treat sugarcane bagasse (SCB) under the condition of 80°C for 2h. NaOH solutions could remove more lignin and obtain higher enzymatic hydrolysis efficiency of SCB than Ca(OH)2 solutions. Compared wi...

    journal_title：Bioresource technology

    pub_type： 杂志文章

    doi：10.1016/j.biortech.2017.08.101

    authors： Chang M,Li D,Wang W,Chen D,Zhang Y,Hu H,Ye X

    更新日期：2017-11-01 00:00:00