Abstract：
:In this study, a novel two-step enzymatic process was firstly established to produce microalgae biodiesel using wet Chlorella biomass. In the first hydrolysis step, to reduce energy consumption and effectively disrupt microalgal cell wall, among cellulase, hemicellulase, papain, lysozyme and pectinase, the highest hydrolysis efficiency (67.52%) was obtained by cellulase at pH 5.0 with enzyme dosage of 200 U/g dry biomass at 40 °C for 12 h. In the second transesterification step, compared with liquid CAL-A/B from Candida antarctica and PLA from Aspergillus oryzae, liquid lipase TL from Thermomyces lanuginosus achieved the highest biodiesel conversion at 81.15:1 (v/w) ethanol/g TFAs ratio in 78-83% water content with 100 PLU/g TFAs lipase loading at 25 °C for 48 h. Moreover, similar results were obtained with three Chlorella species by this process. Overall, this two-step enzymatic process was a green, low-energy and efficient method for cost-effective biodiesel production using wet microalgal biomass.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
He Y,Wu T,Wang X,Chen B,Chen Fdoi
10.1016/j.biortech.2018.08.038subject
Has Abstractpub_date
2018-11-01 00:00:00pages
583-591eissn
0960-8524issn
1873-2976pii
S0960-8524(18)31141-6journal_volume
268pub_type
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