Abstract:
:Heterotrophic microalgae, Aurantiochytrium sp. KRS101 had a large amount of lipid (56.8% total lipids). The cells in the culture medium were easily ruptured due to thin cell wall of Aurantiochytrium sp., which facilitated in-situ fatty acid methyl esters (FAMEs) production directly from biomass. The harvested biomass had a high content of free fatty acids (FFAs), which was advantageous for glycerol-free FAMEs production. FAMEs were directly produced from Aurantiochytrium sp. KRS101 biomass (48.4% saponifiable lipids) using Novozyme 435-catalyzed in-situ esterification in dimethyl carbonate (DMC). DMC was used as a lipid extraction reagent, acyl acceptor and reaction medium. A 433.09mg FAMEs/g biomass was obtained with 89.5% conversion under the optimal condition: DMC to biomass ratio of 5:1 (v/w) and enzyme to biomass ratio of 30% (w/w) at 50°C for 12h. Glycerol could not be detected in the produced FAMEs.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Kim KH,Lee OK,Kim CH,Seo JW,Oh BR,Lee EYdoi
10.1016/j.biortech.2016.03.092subject
Has Abstractpub_date
2016-07-01 00:00:00pages
472-7eissn
0960-8524issn
1873-2976pii
S0960-8524(16)30395-9journal_volume
211pub_type
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