Abstract:
:The objective of this study was to use glycerol generated from the synthesis of biodiesel to study the oleaginous potential of wild yeasts. An initial selection was performed via a rapid and qualitative technique by staining with Sudan Black B. Initially 129 yeasts were present, from which 5 were selected and cultivated in liquid medium containing pure or raw glycerol. The yeast LEB-M3, isolated from the Pantanal, presented lipid content of 20.46% and 56.58% for cultivation in pure and raw glycerol, respectively. This strain was genotypically identified as Candida sp. The fatty acid profile showed predominance of oleic acid (C18:1), 57.35% for cultivation in pure glycerol, and in raw glycerol linoleic acid (C18:2) was predominant (46.0%). It was possible to select a yeast with high lipid concentrations 9.14 g/L and fatty acid profile similar to vegetable oils commonly used in the synthesis of biodiesel.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Duarte SH,de Andrade CC,Ghiselli G,Maugeri Fdoi
10.1016/j.biortech.2013.04.004subject
Has Abstractpub_date
2013-06-01 00:00:00pages
377-81eissn
0960-8524issn
1873-2976pii
S0960-8524(13)00608-1journal_volume
138pub_type
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