Thermo-acido-tolerant phytase production from a soil bacterium in a medium containing rice bran and soybean meal extract.

Abstract:

:A bacterial strain capable of producing a thermo-acido-tolerant phytase was isolated from soil around haystacks and designated as strain PH01. The phytase produced was purified to homogeneity as determined by native PAGE. From SDS-PAGE, it was 30 kDa in size. The purified phytase was a thermo-acido-tolerant enzyme. A complex medium for the PH01 phytase production was developed. The medium, "PheB", was composed of 2% glucose, 0.2% CaCl(2), 0.5% NH(4)NO(3), 0.05% KCl, 0.05% MgSO(4).7H(2)O, 0.001% FeSO(4).7H(2)O, 0.001% MnSO(4).H(2)O in rice bran plus soybean meal extract containing 3% (v/v) phosphate solution (7.3% NaHPO(4)+3.2%KH(2)PO(4), pH 7.2). Cultivation was done at 37 degrees C with aeration for 48 h which produced phytase at 10 U/ml. Exposure of the phytase to 1% bile salt; i.e., taurocholate or deoxycholate, caused less than 15% reduction of activity. Potential application of PH01 phytase as a feed supplement was suggested.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Popanich S,Klomsiri C,Dharmsthiti S

doi

10.1016/s0960-8524(02)00236-5

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

295-8

issue

3

eissn

0960-8524

issn

1873-2976

pii

S0960852402002365

journal_volume

87

pub_type

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