Abstract:
:Nitrilases constitute an important class of hydrolases, having numerous industrial applications. The present work aims to address the production of nitrile hydrolyzing enzymes from Pseudomonas putida MTCC 5110 in a 6l bioreactor. Effect of various physico-chemical conditions and process parameters like pH, temperature, aeration and agitation rates and inducer concentration was studied. Further, the enzyme activity was enhanced by adopting the inducer feeding strategy. Various biochemical engineering parameters pertaining to the cultivation of P. putida in different physico-chemical conditions were reported. Finally, segregation of growth phase from the enzyme production phase allowed significant reduction in total fermentation time.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Naik SC,Kaul P,Barse B,Banerjee A,Banerjee UCdoi
10.1016/j.biortech.2006.11.053subject
Has Abstractpub_date
2008-01-01 00:00:00pages
26-31issue
1eissn
0960-8524issn
1873-2976pii
S0960-8524(06)00651-1journal_volume
99pub_type
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