Studies on the production of enantioselective nitrilase in a stirred tank bioreactor by Pseudomonas putida MTCC 5110.

Abstract:

:Nitrilases constitute an important class of hydrolases, having numerous industrial applications. The present work aims to address the production of nitrile hydrolyzing enzymes from Pseudomonas putida MTCC 5110 in a 6l bioreactor. Effect of various physico-chemical conditions and process parameters like pH, temperature, aeration and agitation rates and inducer concentration was studied. Further, the enzyme activity was enhanced by adopting the inducer feeding strategy. Various biochemical engineering parameters pertaining to the cultivation of P. putida in different physico-chemical conditions were reported. Finally, segregation of growth phase from the enzyme production phase allowed significant reduction in total fermentation time.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Naik SC,Kaul P,Barse B,Banerjee A,Banerjee UC

doi

10.1016/j.biortech.2006.11.053

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

26-31

issue

1

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(06)00651-1

journal_volume

99

pub_type

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