Abstract：
:Two of the six esterases identified in Cucurbita pepo cv. "Eskandrani" were purified to homogeneity using two chromatography steps: anion exchange and gel filtration. The molecular weights of C. pepo esterases EIc and EII were 50,000 +/- 1500 and 68,000 +/- 1900 Da from gel filtration and 47,000 and 66,000 Da from SDS/PAGE, respectively, suggesting a monomeric structure for both enzymes. Esterases EIc and EII had K(m) values of 1.22 and 1.56 mM and pH optima at 9.0 and 8.0, respectively. The substrate specificity of C. pepo esterases EIc and EII were determined for a number of p-nitrophenyl esters, where their affinity toward these substrates were decreased as carbon atom number increased. Esterases EIc and EII had the same temperature optima, 40 degrees C. Thermal stability studies of esterases EIc and EII indicated that half maximal activities of EIc and EII esterases were reached at 55 degrees C and 50 degrees C, while they lost 45%, 51% and 70%, 77% of their activities after 30 and 90 min of incubation at 40 degrees C, respectively. The effect of different metal cations and inhibitors were examined. The inhibition studies revealed that the active sites of the two esterases contain serine and cysteine residues. The characteristics of C. pepo esterases are closely similar to those of microbial esterases used in food processing and food industry.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Fahmy AS,Abo-Zeid AZ,Mohamed TM,Ghanem HM,Borai IH,Mohamed SAdoi
10.1016/j.biortech.2006.11.062subject
Has Abstractpub_date
2008-01-01 00:00:00pages
437-43issue
2eissn
0960-8524issn
1873-2976pii
S0960-8524(07)00002-8journal_volume
99pub_type
杂志文章abstract：:Microalgal biohydrogen (bioH2) has attracted global interest owing to its potential carbon-free source of sustainable renewable energy. Most of previous reviews which focused on microalgal bioH2, have shown unclear differentiation among the metabolic pathways. In this review, investigation of all different metabolic p...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2020.124253
更新日期：2020-12-01 00:00:00
abstract：:This study proposed a novel approach of cultivating aerobic granular sludge (AGS) using intertidal wetland sediment (IWS) as inoculant in MBR for saline wastewater treatment. Granulation was observed in IWS-MBR during start-up, with increased sludge particle size (3.1-3.3 mm) and improved settling property (23.8 ml/g)...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.123715
更新日期：2020-10-01 00:00:00
abstract：:Myxobacteria are soil microorganisms with the ability to break down biological macromolecules due to the secretion of a large number of extracellular enzymes, but there has been no research report on myxobacterial lytic polysaccharide monooxygenases (LPMOs). In this study, two LPMO10s, ViLPMO10A and ViLPMO10B, from my...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.124217
更新日期：2020-12-01 00:00:00
abstract：:A bio-cathode microbial fuel cell (MFC) with tubular membrane was integrated to construct a microbial fuel cell-tubular membrane bioreactor (MFC-TMBR) system, in which the bio-cathode MFC was developed as a biosensor for COD real-time monitoring in TMBR and the performance was analyzed in terms of its current variatio...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.08.033
更新日期：2014-10-01 00:00:00
abstract：:A biorefinery approach was implemented to produce a superantixoident, i.e., astaxanthin, and biofuels, i.e., ethanol and biogas, from the biomass of microalga Haematococcus pluvialis. The hydrolysis of residual biomass obtained from astaxanthin extraction was conducted using α-amylase and glucoamylase for hydrolysis o...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.123100
更新日期：2020-02-29 00:00:00
abstract：:The removal of native acetyl groups from hardwood O-acetyl-glucuronoxylan has a strong effect on physical characteristics, accessibility and structure of this polymer. The removal also has effects on the swelling and ion transport capacity of the cell wall of hardwoods. In this work, a kinetic expression for Eucalyptu...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2008.10.054
更新日期：2009-04-01 00:00:00
abstract：:The high cost of cellulase production presents biggest challenge in biomass deconstruction. Cellulase production by Trichoderma reesei using low cost carbon source is of great interest. In this study, an artificial transcription activator containing the Cre1 binding domain linked to the Xyr1 effector and binding domai...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.10.083
更新日期：2017-01-01 00:00:00
abstract：:The green microalga Haematococcus pluvialis was cultured with different concentrations of NaNO(3) to determine the effect on cell growth and astaxanthin accumulation. The optimum nitrate concentration to obtain astaxanthin and to avoid the cessation of cell division was 0.15 g/l NaNO(3). The ratio chlorophyll a/total ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2004.04.006
更新日期：2005-02-01 00:00:00
abstract：:Five heavy metals namely, arsenic (As), cadmium (Cd), chromium (Cr), lead (Pb) and mercury (Hg) are carcinogenic and show toxicity even at trace amounts, posing threats to environmental ecology and human health. There is an emerging trend of employing microalgae in phycoremediation of heavy metals, due to several bene...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2020.122886
更新日期：2020-05-01 00:00:00
abstract：:Bacillus amyloliquefaciens SH-B10 isolated from deep-sea sediment produces two anti-fungal lipopeptides that were purified by bioactivity-guided fractionation based on acid precipitation, vacuum flash chromatography and semi-preparative HPLC. The two compounds were identified by tandem Q-TOF mass spectroscopy as C16 f...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.06.054
更新日期：2010-11-01 00:00:00
abstract：:Effects of NO3(-)/SO4(2-) ratio on denitrification and sulfate removal efficiency were investigated in model experiments applying haloalkaliphilic bioreactor. The reduction of both substrates performed well at different NO3(-)/SO4(2-) ratios ranging from 17.6 to l.5. The removal rates of nitrate and sulfate were 6 and...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.12.009
更新日期：2014-02-01 00:00:00
abstract：:Performance of a two-chamber microbial fuel cell (MFC) was evaluated with the influence of cathodic dissolved oxygen (DO). The maximum voltage, coulombic efficiency and maximum power density outputs of MFC decreased from 521 to 303 mV, 52.48% to 23.09% and 530 to 178 mW/m(2) with cathodic DO declining. Furthermore, a ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.05.002
更新日期：2014-07-01 00:00:00
abstract：:In order to explore the effects of different mechanical fragmentation on cellulose separation and cellulose polymorphic transformation of wheat straw during alkali treatment, one coarse milled (CM) and two ball milled wheat straw samples (BM30 and BM120) were treated with different NaOH concentrations (1%-10%), and th...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.123535
更新日期：2020-09-01 00:00:00
abstract：:To investigate the effects of viscosity on the mesophilic digestion of Maotai-flavored distiller's grains, a continuous experiment was conducted in a 70 L reactor at organic loading rates of 3, 4, 5, and 6 g VS/(L·d) with and without effluent recirculation. High organic loading rates and continuous effluent recirculat...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.122460
更新日期：2020-02-01 00:00:00
abstract：:1,3-Propanediol (1,3-PDO) production from crude glycerol, a byproduct from biodiesel manufacturing, by Clostridium beijerinckii DSM 791 was studied with corn steep liquor as an inexpensive nitrogen source replacing yeast extract in the fermentation medium. A stable, long-term 1,3-PDO production from glycerol was demon...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.04.020
更新日期：2016-07-01 00:00:00
abstract：:The larvicidal effects of the essential oils extracted from the leaves of Cryptomeria japonica at different ages (58, 42, and 26 years old) against 2 mosquito species, Aedes aegypti and Aedes albopictus, were studied. The analysis of major constituents of these essential oils was also investigated. Results obtained fr...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.11.060
更新日期：2009-01-01 00:00:00
abstract：:This study aims to efficiently use hemicellulose-based biomass for ABE (acetone-butanol-ethanol) production by co-fermentation with starch-based biomass. Two processes were investigated: (I) co-fermentation of sugars derived from hemicellulose and starch in a mixture of barley straw and grain that was pretreated with ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.12.005
更新日期：2015-03-01 00:00:00
abstract：:Lignocellulosic biomass is a sustainable source of renewable substrate to produce low carbon footprint energy and materials. Biomass conversion is usually performed in two steps: a biomass pretreatment for improving cellulose accessibility followed by enzymatic hydrolysis of cellulose. In this study we investigated th...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.03.045
更新日期：2019-06-01 00:00:00
abstract：:Biochemical properties of granules are of vital importance to UASB performance. This study characterized the granules cultivated at different COD/SO42- ratios to elucidate the influence of sulfidogenesis on starch wastewater (1000 mg-COD L-1) biodegradation kinetics and process stability. Suitable sulfate addition enr...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.02.055
更新日期：2018-05-01 00:00:00
abstract：:Multi-electrode bioelectrochemical treatment system (ME-BET; membrane less) consisting of six electrode assemblies (E1-E6) was designed and fabricated for the treatment of complex chemical based wastewater with high salt concentration. The performance was compared with single electrode assembly BET reactor (SE-BET). E...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.05.048
更新日期：2017-10-01 00:00:00
abstract：:Fermentation of sulfuric acid treated corn fiber hydrolysate (SACFH) inhibited cell growth and butanol production (1.7+/-0.2g/L acetone butanol ethanol or ABE) by Clostridium beijerinckii BA101. Treatment of SACFH with XAD-4 resin removed some of the inhibitors resulting in the production of 9.3+/-0.5 g/L ABE and a yi...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.09.087
更新日期：2008-09-01 00:00:00
abstract：:Wood catalytic pyrolysis using calcium-based materials was studied in an auger reactor at 450°C. Two different catalysts, CaO and CaO·MgO were evaluated and upgraded bio-oils were obtained in both cases. Whilst acidity and oxygen content remarkable decrease, both pH and calorific value increase with respect to the non...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.03.146
更新日期：2014-06-01 00:00:00
abstract：:This paper will consider the influence of the temperature of autohydrolysis or hydrothermal process from Paulownia fortunei L. to obtain a valuable liquid phase and a suitable solid phase to produce pulp. The solid phase resulting of autohydrolysis was subjected to organosolv pulping process and formed paper sheets, a...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.01.028
更新日期：2008-03-01 00:00:00
abstract：:The compositions of food wastes and their co-gasification producer gas were compared with the existing data of sewage sludge. Results showed that food wastes are more favorable than sewage sludge for co-gasification based on residue generation and energy output. Two decentralized gasification-based schemes were propos...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.07.017
更新日期：2016-10-01 00:00:00
abstract：:The global economy is threatened by the depletion of fossil resources and fluctuations in fossil fuel prices, and thus it is necessary to exploit sustainable energy sources. Carbon-neutral fuels including bio-oil obtained from biomass pyrolysis can act as alternatives to fossil fuels. Co-pyrolysis of lignocellulosic b...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2020.123473
更新日期：2020-08-01 00:00:00
abstract：:A bench scale biofilter packed with compost and wood chips seeded with potential DMS degrading culture (Bacillus sphaericus) could efficiently remove DMS from ambient air with removal efficiency (RE%) of 71 ± 11 at an effective bed contact time (EBCT) of 360 ± 20s with loading rate in the range of 4-28 gDMS/m(3)/h. Fu...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.04.100
更新日期：2013-08-01 00:00:00
abstract：:Constructing anti-fouling and self-cleaning membrane surfaces based on covalent attachment of trypsin on poly(methacrylic acid)-graft-polyethersulfone (PMAA-g-PES) membrane was reported. The carboxylic acid groups enriched on asymmetric PMAA-g-PES membrane surface were activated with 1-ethyl-(3-3-dimethylaminopropyl)-...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.08.030
更新日期：2011-01-01 00:00:00
abstract：:p-Chloronitrobenzene (p-CNB) is a persistent refractory and toxic pollutant with a concentration up to 200 mg/L in industrial wastewater. Here, a super-fast removal rate was found at 0.2-0.8 V of external voltage over a p-CNB concentration of 40-120 mg/L when a bioelectrochemical technology is used comparing to the na...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.10.068
更新日期：2018-02-01 00:00:00
abstract：:Strain HNR, isolated from a Membrane Bioreactor (MBR), demonstrates a surprising ability to convert ammonium to nitrogen gas under aerobic conditions while growing heterotrophically. On the basis of phylogenetic analysis of the 16S rRNA gene sequence, strain HNR was related to Acinetobacter calcoaceticus (98.9% identi...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.02.043
更新日期：2010-07-01 00:00:00
abstract：:Lignin is an important raw material for the sustainable biorefineries and also the forerunner of high-value added products, such as biocomposite for chemical, pharmaceutical and cement industries. Oil palm empty fruit bunches (OPEFB) were used for lignin preparation by successive treatment with 1% (w/w) H2SO4 at 121°C...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.07.018
更新日期：2015-10-01 00:00:00
