Abstract：
:The high cost of cellulase production presents biggest challenge in biomass deconstruction. Cellulase production by Trichoderma reesei using low cost carbon source is of great interest. In this study, an artificial transcription activator containing the Cre1 binding domain linked to the Xyr1 effector and binding domains was designed and constitutively overexpressed in T. reesei RUT C30. The recombinant strain T. reesei zxy-2 displayed constitutive cellulase production using glucose as a sole carbon source, and the production titer was 12.75-fold of that observed with T. reesei RUT C30 in shake flask culture. Moreover, FPase and xylanase titers of 2.63 and 108.72IU/mL, respectively, were achieved using glucose as sole carbon source within 48h in a 7-L fermenter by batch fermentation using T. reesei zxy-2. The crude enzyme obtained was used to hydrolyze alkali pretreated corn stover, and a high glucose yield of 99.18% was achieved.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zhang X,Li Y,Zhao X,Bai Fdoi
10.1016/j.biortech.2016.10.083subject
Has Abstractpub_date
2017-01-01 00:00:00pages
317-322eissn
0960-8524issn
1873-2976pii
S0960-8524(16)31497-3journal_volume
223pub_type
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