Enhancement of lipid accumulation by oleaginous yeast through phosphorus limitation under high content of ammonia.

Abstract:

:Low concentrations of acetic acid were used as carbon source to cultivate Cryptococcus curvatus MUCL 29819 for lipid production under high content of ammonia. Phosphorus limitation combined with initial pH regulation (pH = 6) weakened inhibition of free ammonia and promoted lipid accumulation. In batch cultivation, the produced lipid content and yield was 30.3% and 0.92 g/L, higher than those under unlimited condition (18.3% and 0.64 g/L). The content of monounsaturated fatty acid also increased from 37.3% (unlimited condition) to 45.8% (phosphorus-limited condition). During sequencing batch cultivation (SBC), the lipid content reached up to 51.02% under phosphorus-limited condition while only 31.88% under unlimited condition, which can be explained by the higher conversion efficiency of the carbon source to lipid. The total energy consumption including lipid extraction, transesterification and purification was 7.47 and 8.33 GJ under phosphorus-limited and unlimited condition, respectively.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Huang X,Luo H,Mu T,Shen Y,Yuan M,Liu J

doi

10.1016/j.biortech.2018.04.063

subject

Has Abstract

pub_date

2018-08-01 00:00:00

pages

9-14

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(18)30585-6

journal_volume

262

pub_type

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