Abstract：
:In this work, a simple, inexpensive, and efficient method of preparing immobilized lipase is presented. The lipase originating from a newly isolated indigenous strain Burkholderia sp. C20 was immobilized onto cellulose nitrate (CN) membrane via filtration. The CN-immobilized lipase was able to retain 60% of its original activity after repeated uses for nine times. The thermal stability of the lipase was also slightly improved after immobilization. The optimal reaction conditions of CN-lipase were pH 9.0 and 55 degrees C, which are similar to those for the suspended lipase. Both suspended and immobilized lipase could hydrolyze the six oil substrates examined, while immobilized lipase displayed less specificity over the oil substrates. Kinetic analysis shows that the dependence of lipolytic activity of both suspended and immobilized lipase on oil substrate concentration can be described by Michaelis-Menten model with good agreement. The estimated kinetic constants for suspended lipase (v(max)=243.9 U/mg, K(m)=0.024 mM) and immobilized lipase (v(max)=32.8 U/mg, K(m)=5.61 mM) were quite different. Employment of immobilization seemed to result in a decrease in v(max) and an increase in K(m), most likely due to the mass transfer resistance arising from formation of micelles during the lipase immobilization process.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Liu CH,Chang JSdoi
10.1016/j.biortech.2007.04.011subject
Has Abstractpub_date
2008-04-01 00:00:00pages
1616-22issue
6eissn
0960-8524issn
1873-2976pii
S0960-8524(07)00329-Xjournal_volume
99pub_type
杂志文章abstract：:The objective of this study was to determine the specific energy requirements for the compression of fractionated sun-cured and dehydrated alfalfa chops, when subjected to different pressures and holding times. The compression behavior of fractionated sun-cured and dehydrated alfalfa chops was studied using a single c...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2005.11.032
更新日期：2007-01-01 00:00:00
abstract：:This study investigated the feasibility of using recovered nutrients from hydrothermal carbonization (HTC) for cultivation of microalga Chlorella vulgaris. Different dilution multiples of 50, 100 and 200 were applied to the recycled process water from HTC and algal growth was compared among these media and a standard ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.09.062
更新日期：2012-12-01 00:00:00
abstract：:Pavlova sp. was employed to evaluate the efficiency of different lipid extraction methods. The microalgal crude lipids content determined using the mixed solvent with ultrasonic method was 44.7 wt.%. The triglyceride content obtained by the mixed solvent method was 15.6 wt.%. The extraction yield was the FAME yield di...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.08.064
更新日期：2011-11-01 00:00:00
abstract：:This paper reports accurate measurements of the radiation characteristics and optical properties of Nannochloropsis oculata in the photosynthetically active radiation (PAR) region. These marine microalgae were grown in 2 cm thick culture bottles with vented caps exposed, on one side, to either white fluorescent light ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.03.058
更新日期：2013-06-01 00:00:00
abstract：:This study examined the production of cellulose nanocrystals from microcrystalline wood cellulose, Avicel and recycled pulp of wood pulp using sono-chemical-assisted hydrolysis. Two hydrolysis systems: deionized water and maleic acid were evaluated. In deionized water, Avicel produced cellulose nanocrystals with avera...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2008.09.062
更新日期：2009-04-01 00:00:00
abstract：:Sludge is produced during wastewater treatment as a residue containing most insoluble and adsorbed soluble impurities in wastewaters. This paper summarized the currently available review papers on sludge treatments and proposed the research trends based on the points raised therein. On partition aspect, sludge product...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2017.07.070
更新日期：2017-11-01 00:00:00
abstract：:The major operational problem associated with membrane bioreactors (MBR) is membrane fouling, for which extracellular polymeric substances (EPS) are primarily responsible. In this work both the soluble and bound EPS (i.e. SMP and EPS) produced in an MBR system operating under sludge retention times (SRT) of 10, 15, 20...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.01.053
更新日期：2009-07-01 00:00:00
abstract：:Three models (blocking laws, combined and resistance-in-series) were applied to identify the prevailing fouling mechanisms in a submerged membrane in an up-flow anaerobic sludge blanket reactor treating municipal wastewater. Experimental runs were carried out at lab-scale with filtration periods of 4 and 10 min, follo...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.08.017
更新日期：2018-11-01 00:00:00
abstract：:This study evaluates the use of engineered biochar as a heterogeneous solid acid catalyst for transesterification of algal oil derived from a native microalgal consortium. Biochar derived from sugarcane bagasse, coconut shell, corncob and peanut shell were evaluated for catalytic activity following surface modificatio...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.123392
更新日期：2020-08-01 00:00:00
abstract：:The continuous fast pyrolysis of soybean oil in a pilot plant was investigated. The experimental runs were carried out according to an experimental design alternating the temperature (from 450 to 600 degrees C) and the concentration of water (from 0% to 10%). The liquid products were analyzed by gas chromatography and...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.07.059
更新日期：2009-12-01 00:00:00
abstract：:In order to evaluate the hydrothermal treatment (HTT) in enhancing compost stability and maturity of lignocellulosic agricultural residues, a bin-scale (90 L) composting of rice straw with and without "HTT" was performed. The rice straw compost product with "HTT" after 6 weeks of composting can be considered stable an...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.10.083
更新日期：2014-01-01 00:00:00
abstract：:Microbial fuel cell (MFC) cathodes rapidly foul when treating domestic wastewater, substantially reducing power production over time. Here a wipe separator was chemically bonded to an activated carbon air cathode using polyvinylidene fluoride (PVDF) to mitigate cathode fouling and extend cathode performance over time....
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.10.109
更新日期：2018-02-01 00:00:00
abstract：:In this work, multiple reuses of Rhodococcus ruber TH3 free cells for the hydration of acrylonitrile to produce acrylamide in a membrane dispersion microreactor were carried out. Through using a centrifuge, the reactions reached 39.9, 39.5, 38.6 and 38.0wt% of the final acrylamide product concentration respectively wi...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.03.134
更新日期：2015-01-01 00:00:00
abstract：:A process for the fermentative production of propionic acid from whole wheat flour using starch and gluten as nutrients is presented. Hydrolysis of wheat flour starch using amylases was optimized. A batch fermentation of hydrolysate supplemented with various nitrogen sources using Propionibacterium acidipropionici NRR...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.12.154
更新日期：2013-02-01 00:00:00
abstract：:Fatty acid ethyl esters (FAEEs) are attractive biofuel molecules. Conventional FAEEs production process uses triglycerides and ethanol as feedstocks and is sensitive to water contents. In this work, we show that the oleaginous yeast Rhodosporidium toruloides cells are capable of converting lipids into FAEEs intracellu...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.10.023
更新日期：2013-12-01 00:00:00
abstract：:Efficient nitrogen removal from domestic sewage was obtained in a sequencing batch biofilm reactor (SBBR) operated in alternate anaerobic/oxic/anoxic (A/O/A) mode. For the influent total nitrogen (TN) ranging from 30.0 to 63.3 mg N·L-1, the average nitrogen removal efficiency was 90.3 ± 2.9% with removal rate of 42.3-...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.121665
更新日期：2019-10-01 00:00:00
abstract：:One way envisioned to overcome part of the issues biodiesel production encounters today is to develop a simple, economically viable and eco-friendly process for the extraction of lipids from microalgae. This study investigates the lipid extraction efficiency from the microalga Chlamydomonas reinhardtii as well as the ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.01.139
更新日期：2018-06-01 00:00:00
abstract：:Biochar is traditionally used to improve soil properties in arable land and as adsorbent or precursor of activated carbon in wastewater treatment. Recent advances have shown biochar potentials in enhancing productions of biofuels and chemicals such as bio-ethanol, butanol, methane, hydrogen, bio-diesel, hydrocarbons a...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2019.122252
更新日期：2020-01-01 00:00:00
abstract：:Considering the variations of optimal light intensity required by microalgae cells along with growth phases, growth-phase light-feeding strategies were proposed and verified in this paper, aiming at boosting microalgae lipid productivity from the perspective of light conditions optimization. Experimental results demon...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.02.045
更新日期：2018-05-01 00:00:00
abstract：:1-Alkenes are traditionally used as basic chemicals with great importance. Biosynthetic 1-alkenes also have the potential to serve as biofuels. In this study, we engineered a Pseudomonas lipolytic microbial platform for 1-alkene production using hydrophobic substrate as sole carbon source. Fatty acid decarboxylase Und...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.04.119
更新日期：2018-09-01 00:00:00
abstract：:Clostridium, Tetrathiobacter and Desulfovibrio species are identified as suitable biocatalysts for treating organic-rich and sulfate-laden wastewater. Results from this study show that the power generation was much higher under alkaline conditions, i.e., pH of 8 when compared to neutral and acidic conditions. The effe...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.123110
更新日期：2020-03-02 00:00:00
abstract：:Decolorization and degradation of polyazo dye Direct Black 22 was carried out by distillery spent wash degrading mixed bacterial consortium, DMC. Response surface methodology (RSM) involving a central composite design (CCD) in four factors was successfully employed for the study and optimization of decolorization proc...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2006.12.033
更新日期：2008-02-01 00:00:00
abstract：:Knowledge of the microbiology of dinitrogen (N2)-fixing bacteria in compost rich in de-inking paper sludge (DPS) is limited. Dinitrogen (N2)-fixing bacteria from DPS composts were isolated and studied for their N2-fixing activity in vitro and in vivo. Two Gram-negative N2-fixing isolates were identified as Pseudomonas...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2005.04.041
更新日期：2006-05-01 00:00:00
abstract：:This study examine ways to make biorefinery lignin accessible for anaerobic digestion. The raw material was the residue after removing carbohydrates by Wet Explosion pretreatment at 190 °C and 7.5% O2 followed by enzymatic hydrolysis. The residual solid was mainly composed of lignin and was the raw material for a seco...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.122537
更新日期：2020-02-01 00:00:00
abstract：:A genetic algorithm has been used to optimize the composition of the culture medium for growing the microalga Nannochloropsis gaditana, based on the nutrients composition of the commercial medium ALGAL. This strategy was carried out through the implementation of 270 experiments spread over nine generations, which allo...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.11.057
更新日期：2015-02-01 00:00:00
abstract：:The effect of dosing a metal limited anaerobic sludge blanket (UASB) reactor with a metal pulse on the methanogenic activity of granular sludge has thus far not been successfully modeled. The prediction of this effect is crucial in order to optimize the strategy for metal dosage and to prevent unnecessary losses of re...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.07.053
更新日期：2010-12-01 00:00:00
abstract：:Cross-linked enzyme aggregates (CLEAs) are reported for the first time for a recombinant acetyl xylan esterase (AXE) from Bacillus pumilus. With this enzyme, CLEAs production was most effective using 3.2M (80%-saturation) ammonium sulfate, followed by cross-linking for 3h with 1% (v/v) glutaraldehyde. Particle size wa...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.08.016
更新日期：2010-01-01 00:00:00
abstract：:The composting of chicken manure and sawdust has been investigated in forced-aeration composting systems at aeration rates of 0.3, 0.5, and 0.7 l min(-1)kg(-1) organic matter (OM), corresponding to pile A, pile B, and pile C. The temperature exceeding 55 degrees C in pile A and pile B remained more than three consecut...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.10.027
更新日期：2010-03-01 00:00:00
abstract：:The effect of mesophilic anaerobic digestion (AD) on the resistome profile of manures from two different dairy farms was evaluated using a metagenomic approach. A total of 187 unique Antibiotic resistance genes (ARGs) for 17 different classes of antibiotics were detected in raw (undigested) manures. The results indica...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.123889
更新日期：2020-11-01 00:00:00
abstract：:In this study, a pilot-scale anaerobic dynamic membrane bioreactor was operated for 142days for treating landfill leachate. Under stable operation, average COD removal efficiency of 62.2% was achieved when the reactor was fed with the raw leachate containing total ammonium concentration above 3000mg/L and COD above 13...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.03.014
更新日期：2014-06-01 00:00:00
