Abstract:
:A proteomic approach was used to identify proteins involved in Cr(VI) stress response of Pseudomonas aeruginosa to toxic Cr(VI). Cytosolic and membrane fractions from bacteria exposed to 300 mg l(-1) Cr(VI) were prepared, 2D gel electrophoresis in combination with MALDI-TOF MS and LC-MS/MS was used to identify proteins whose expression level increased or decreased upon exposure to Cr(VI). Overexpressed proteins include stress proteins, proteins involved in protein biosynthesis, proteins responsible for energy production, proteins involved in free radicals detoxification by the glutathione system, outer membrane proteins, MucD, while downregulated proteins were isocitrate dehydrogenase and 30S ribosomal protein S1. Under Cr(VI) exposure, upregulation of MucD (role in exopolysaccharide production) and outer membrane proteins concluded that bacteria have access to more than one resistance mechanism against toxic metal ions. We propose that the mechanisms of Cr(VI) resistance include production of exopolysaccharide and complexing of metal ions outside the cell.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Kiliç NK,Stensballe A,Otzen DE,Dönmez Gdoi
10.1016/j.biortech.2009.11.008subject
Has Abstractpub_date
2010-04-01 00:00:00pages
2134-40issue
7eissn
0960-8524issn
1873-2976pii
S0960-8524(09)01512-0journal_volume
101pub_type
杂志文章abstract::Sulfate-reducing fluidized-bed bioreactor (FBR) fed with ethanol-lactate mixture was operated at 35 degrees C for 540 days to assess mine wastewater treatment, biological hydrogen sulfide production capacity and acetate oxidation kinetics. During the mine wastewater treatment period with synthetic wastewater, the sulf...
journal_title:Bioresource technology
pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
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journal_title:Bioresource technology
pub_type: 杂志文章,收录出版
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journal_title:Bioresource technology
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doi:10.1016/j.biortech.2010.05.021
更新日期:2011-01-01 00:00:00
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journal_title:Bioresource technology
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doi:10.1016/j.biortech.2018.07.110
更新日期:2018-11-01 00:00:00
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journal_title:Bioresource technology
pub_type: 杂志文章
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更新日期:2011-04-01 00:00:00
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