Abstract:
:Acetate can be used as carbon feedstock for the production of 3-hydroxypropionic acid (3-HP), but the production level was low due to inefficient cell growth on acetate. To better utilize acetate, a two-stage strategy, whereby glucose is used for cell growth and acetate for 3-HP formation, was attempted. Dissected malonyl-CoA reductase of Chloroflexus aurantiacus, alone or along with acetyl-CoA carboxylase and/or transhydrogenase, was overexpressed, and by-products formation pathway, glyoxylate shunt (GS) and gluconeogenesis were modified. When GS or gluconeogenesis was disrupted, cell growth on glucose was not hampered, while on acetate it was completely abolished. Consequently, 3-HP production, at production stage, were low, though 3-HP yield on acetate was increased, especially in the case of aceA deletion. In two-stage bioreactor, strain with upregulated GS produced 7.3 g/L 3-HP with yield of 0.26 mol/mol acetate. This study suggests that two-stage cultivation is a good strategy for 3-HP production from acetate.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Lama S,Kim Y,Nguyen DT,Im CH,Sankaranarayanan M,Park Sdoi
10.1016/j.biortech.2020.124362subject
Has Abstractpub_date
2021-01-01 00:00:00pages
124362issue
Pt Aeissn
0960-8524issn
1873-2976pii
S0960-8524(20)31636-9journal_volume
320pub_type
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