Enhancing biomass and ethanol production by increasing NADPH production in Synechocystis sp. PCC 6803.

Abstract:

:This study demonstrates that increased NADPH production can improve biomass and ethanol production in cyanobacteria. We over-expressed the endogenous zwf gene, which encodes glucose-6-phosphate dehydrogenase of pentose phosphate pathway, in the model cyanobacterium Synechocystis sp. PCC 6803. zwf over-expression resulted in increased NADPH production, and promoted biomass production compared to the wild type in both autotrophic and mixotrophic conditions. Ethanol production pathway including NADPH-dependent alcohol dehydrogenase was also integrated with and without zwf over-expression. Excessive NADPH production by zwf over-expression could improve both biomass and ethanol production in the autotrophic conditions.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Choi YN,Park JM

doi

10.1016/j.biortech.2016.02.056

subject

Has Abstract

pub_date

2016-08-01 00:00:00

pages

54-57

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(16)30189-4

journal_volume

213

pub_type

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