Abstract:
PURPOSE:The traditional medium for storing corneas in European Eye Banks is Gibco's MEM (Eagle's) with Earle's salts and Hepes containing 2% fetal calf serum, glutamine and antimycotics. Although serum-free MegaCell MEM has been reported to be more suitable for this purpose, it contains components of animal origin that potentially pose health risks to corneal recipients. The possibility of removing or replacing these components has therefore been investigated. METHODS:A MegaCell basal medium (DME) and a formulation of this (MegaCell DCS), which contains no components of animal origin, have been prepared. The viability of the endothelial, epithelial and stromal cells of corneas held in these media has been assessed, their stress levels monitored and water content determined. RESULTS:The endothelial cell count and morphology of corneas held in MegaCell DME and DCS for 30 days remained little changed. Their epithelial and stromal cells also retained their ability to proliferate in culture. Neither DME nor DCS prevented corneal swelling but the lack of endogenous MMP-2 activation indicated that the corneas were not subject to metabolic stress. CONCLUSION:MegaCell DCS is an animal product-free medium formulated for corneal storage. The quality of corneas held in this medium is similar to those held in MegaCell MEM.
journal_name
Ophthalmic Resjournal_title
Ophthalmic researchauthors
Smith VA,Johnson Tdoi
10.1159/000246576subject
Has Abstractpub_date
2010-01-01 00:00:00pages
33-42issue
1eissn
0030-3747issn
1423-0259pii
000246576journal_volume
43pub_type
杂志文章abstract::Topcon Tokyo manufactured the first series of SL-45 in 1980 and a second in 1982/83, which were completed in Bonn with all technical improvements developed so far. In 1987, the SL-45B was manufactured which included all previously added improvements. To compare both camera types, we carried out a test with 10 voluntee...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267057
更新日期:1990-01-01 00:00:00
abstract:PURPOSE:In this investigation the authors studied the effect of dapiprazole, an alpha-adrenergic blocking agent, on aqueous humor dynamics in patients affected with pigmentary glaucoma. METHODS:Dapiprazole 0.5% eye drops were administered 3 times daily as adjunctive therapy to 16 pigmentary glaucoma patients in additi...
journal_title:Ophthalmic research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000267920
更新日期:1996-01-01 00:00:00
abstract:AIMS:The purpose of this paper was to determine the lacrimal concentration of IL-1α and MMP-9 in patients with active ocular rosacea before and after systemic treatment with azithromycin or doxycycline. METHODS:After 4 weeks of therapy with azithromycin (500 mg/day, 3 days a week PO) or doxycycline (200 mg/day PO), la...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000489092
更新日期:2018-01-01 00:00:00
abstract:AIM/BACKGROUND:A 1-bp deletion (1147delA) in the SAG (also known as arrestin or S-antigen) gene is the most frequently seen mutation in Japanese patients suffering from Oguchi disease, a recessively inherited stationary night blindness. We investigated macular function in a patient with Oguchi disease with the 1147delA...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000325024
更新日期:2011-10-01 00:00:00
abstract::Substance P (SP) immunoreactivity was demonstrated using the indirect immunofluorescence technique in one normal and one retinoblastomatous human retina. In the normal retina SP immunoreaction was located in nerve fibres but not in the neurons in the inner plexiform layer. A similar location was observed in the histol...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265276
更新日期:1983-01-01 00:00:00
abstract:INTRODUCTION:This study aimed to evaluate the longitudinal changes in retinal layers thickness in patients treated with hydroxychloroquine and no signs of retinal toxicity. METHODS:Longitudinal retrospective study of patients taking hydroxychloroquine followed in a hydroxychloroquine retinal toxicity screening program...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000511592
更新日期:2020-09-15 00:00:00
abstract::We investigated the effects of hyperthermia on the healing process of experimental Pseudomonas corneal ulceration (PCU). Hartley guinea pigs were used to develop animal models of PCU. As a heat source, disposable chemical pocket warmers were applied. The healing process of PCU was compared between the heat-treated cor...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000078780
更新日期:2004-07-01 00:00:00
abstract::Animal models are indispensable for pharmaceutical investigations. However, investigators often have difficulty choosing the appropriate modal for their research. To provide a comprehensive and convenient source of information about animal models of proliferative vitreoretinopathy (PVR), the current review sorted and ...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000488492
更新日期:2018-01-01 00:00:00
abstract:PURPOSE:To analyze chloroquine-induced morphological changes in the retinal pigment epithelium (RPE) and Bruch's membrane (BM). METHODS:Retina-choroid complexes of chloroquine-treated Long-Evans rats were analyzed by electron microscopy. RESULTS:Intercellular spaces between the RPE cells and BM were enlarged. Residua...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000090268
更新日期:2006-01-01 00:00:00
abstract:PURPOSE:To evaluate the long-term outcome of trabeculectomy with intra- and postoperative 5-fluorouracil (5-FU) application in glaucoma. METHODS:Eighty-six patients with glaucoma planned for primary trabeculectomy with 5-FU and a minimum follow-up of 3 years were retrospectively analyzed. Success rates, postsurgical 5...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000464446
更新日期:2017-01-01 00:00:00
abstract::A method for microscopical observation and quantification of corneal neovascularization in the living mouse is described. Measurements of the corneal vasculature were made on still-mode video-pictures of the cornea. The validity of the method was shown by the high correlation between the morphometric measurements and ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267097
更新日期:1991-01-01 00:00:00
abstract::The gut microbiome has important physiological functions and plays an indispensable role in the human body. Currently, there are an increasing number of studies revealing the close correlation between dysbiosis of the gut microbiome and a variety of autoimmune diseases, including autoimmune uveitis. This brief review ...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000510212
更新日期:2020-07-16 00:00:00
abstract:AIMS:To investigate whether allogeneic limbal mesenchymal stem cell (LMSC) therapy affects corneal healing after a severe chemical burn and whether the route of administration of LMSCs differs in its therapeutic effect in this respect. METHODS:A total of 60 Sprague-Dawley rats with clinically proven alkali injury were...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000368659
更新日期:2015-01-01 00:00:00
abstract::This study examined and characterized the distribution and kinetics of the inflammatory cell response to pseudomonas ocular challenge in susceptible C57BL/6J and resistant DBA/2J mice. Initially, in the cornea, the number of neutrophilic leukocytes (PMNs), macrophages/monocytes or lymphocytes was 2-2.5 times greater i...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267142
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:We assessed bleb morphology and the intraocular pressure (IOP)-lowering effect of trabeculectomy with ologen compared to mitomycin C (MMC) in juvenile open-angle glaucoma (JOAG). METHODS:This is a prospective interventional comparative study conducted on 40 eyes (20 patients) with medically uncontrolled JOA...
journal_title:Ophthalmic research
pub_type: 杂志文章,随机对照试验
doi:10.1159/000456719
更新日期:2017-01-01 00:00:00
abstract::Ocular pneumoplethysmography was performed before and after 1,737 carotid endarterectomies, 82% of which were performed for carotid lesions of hemodynamic consequence. Preoperative ocular hypoperfusion without ischemia was associated with at least transient postoperative ocular hyperemia. A difference in ocular volume...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000055558
更新日期:1999-01-01 00:00:00
abstract:OBJECTIVES:To evaluate the efficacy of postenucleation saline wash and the effect of different antimicrobial agents on the microbial contamination of donor eyes. METHODS:Two hundred donor eyes were given saline wash and treated with any one of the following 5 randomly selected antimicrobial solutions: 1% povidone-iodi...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000095772
更新日期:2006-01-01 00:00:00
abstract::In order to know the correlation of a drug with high affinity to melanin in the iris ciliary body with the amount of melanin contained in the iris ciliary tissues, an in vivo study was performed on pigmented rabbits with brown or blue irises and albino rabbits. The melanin concentrations in the iris ciliary bodies of ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267404
更新日期:1994-01-01 00:00:00
abstract::HY-1 genotype chick lenses display epithelial hyperplasia and associated lens opacities. Membrane proteins from HY-1 and normal chick lenses were separated by isoelectric focusing, and 45Ca2+ binding capacities were determined. Markedly higher (congruent to 900-fold) binding of 45Ca2+ was observed in high molecular we...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265397
更新日期:1985-01-01 00:00:00
abstract::The purpose of this study was to determine the expression of metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in the aqueous humour of patients with exfoliation syndrome (XFS). XFS and control samples were analysed for their MMP content by zymography and for their tissue inhibitors by ELISA. In XFS eyes, ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000063661
更新日期:2002-05-01 00:00:00
abstract::Cataractous lens images taken with different camera types for retroillumination photography were compared. Images taken by conventional photo slitlamp and fundus cameras were not suitable for image analysis. Retroillumination photography with a conventional fundus camera with additional polarizing filters seemed to be...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267063
更新日期:1990-01-01 00:00:00
abstract::Correlation proton nuclear magnetic resonance spectroscopy was performed on light- and dark-adapted rabbit retinas to elucidate real-time hexose monophosphate shunt (HMPS) activity. Light significantly stimulated retinal HMPS initially, which consumed 17% more glucose than that in dark-adapted retinas. The glucose con...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265511
更新日期:1987-01-01 00:00:00
abstract:PURPOSE:Single-field non-mydriatic fundus photography (NMFP) has been used to detect diabetic retinopathy (DR) in many studies; however, its value in a general clinical setting has not been established. Here we performed a meta-analysis to evaluate its diagnostic effectiveness. METHOD:We systematically searched PubMed...
journal_title:Ophthalmic research
pub_type: meta分析
doi:10.1159/000499106
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:In this study, we used a rat model of retinal detachment (RD) to investigate the effects of transient receptor potential mucolipin 1 (TRPML1) on photoreceptor cells and the underlying mechanism. METHODS:An RD model was established by subretinal injection of sodium hyaluronate, and mucolipin synthetic agonist...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000512104
更新日期:2020-10-07 00:00:00
abstract:BACKGROUND:The aim of this work was to compare the efficacy of intravitreal dexamethasone implant (Ozurdex) and intravitreal ranibizumab (Lucentis) in the treatment of macular edema (ME) caused by retinal vein occlusion (RVO). METHODS:Thirty-two ME cases treated with Ozurdex and 32 ME cases treated with ranibizumab we...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000458534
更新日期:2017-01-01 00:00:00
abstract::To evaluate the possible role of nitric oxide in the pathogenesis of exogenous prostaglandin (PG) induced aqueous flare elevation, we examined the effect of NG-nitro-L-arginine methyl ester (L-NAME), an L-arginine analogue acting as a specific inhibitor of nitric oxide synthase, on the elevation of aqueous flare in pi...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267912
更新日期:1996-01-01 00:00:00
abstract::Autofluorescence and transmission values of the lens for blue-green light were determined at two locations with the use of identical fluorophotometers: in Coimbra, Portugal, in 44 healthy controls, 15 haemodialysis and 11 renal failure patients, and in Leiden, The Netherlands, in 57 healthy controls, 23 haemodialysis ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000266736
更新日期:1988-01-01 00:00:00
abstract::The influence of nonselective and selective alpha-adrenergic agonists on intraocular pressure (IOP) was studied in isolated arterially perfused rabbit eyes. Drugs were given intra-arterially or topically. With either route of administration, no initial hypertensive phase was observed after l-epinephrine, l-norepinephr...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265400
更新日期:1985-01-01 00:00:00
abstract::Cellular immune responses in retinal autoimmunity have scarcely been studied in rabbits. An effective isolation of rabbit peripheral blood lymphocytes is difficult to achieve and requires specific conditions. This hampers especially the assay of antigen-induced lymphocyte transformation. We have improved the lymphocyt...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000266934
更新日期:1989-01-01 00:00:00
abstract:PURPOSE:To investigate therapeutic ocular surface medium (TOSM), a potential physiological tear replacement therapy, for persistent epithelial defect (PED). METHODS:11 eyes of 10 patients with PED for ≥ 2 weeks without improvement despite conventional treatment were enrolled in a prospective pilot study of TOSM over 1...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000355065
更新日期:2014-01-01 00:00:00