Abstract:
:We investigated the effects of hyperthermia on the healing process of experimental Pseudomonas corneal ulceration (PCU). Hartley guinea pigs were used to develop animal models of PCU. As a heat source, disposable chemical pocket warmers were applied. The healing process of PCU was compared between the heat-treated corneas and the control corneas. The severity of infection and the degree of angiogenesis were classified by a clinical scoring system. The animals were euthanized 14 days after infection and the corneas were submitted for histopathological examination. The expression of vascular endothelial growth factor (VEGF) was examined immunohistochemically. Comparative reverse transcription polymerase chain reaction was performed to measure the expression level of VEGF in the cornea. Hyperthermia significantly promoted corneal epithelization and neovascularization in the PCU model. Heat treatment significantly decreased the number of viable Pseudomonas organisms present in PCU. On immunohistochemistry, the heated cornea demonstrated more intense staining for VEGF. Comparative reverse transcription polymerase chain reaction showed upregulation of the expression level of VEGF mRNA in the heat-treated cornea. Hyperthermia accelerated the healing process of PCU with increased corneal neovascularization. Angiogenesis may play an important role in the PCU healing process, which is enhanced by the heat treatment.
journal_name
Ophthalmic Resjournal_title
Ophthalmic researchauthors
Nanbu PN,Wakabayashi T,Yamashita R,Hayashi H,Hisano S,Oshika Tdoi
10.1159/000078780subject
Has Abstractpub_date
2004-07-01 00:00:00pages
218-25issue
4eissn
0030-3747issn
1423-0259pii
78780journal_volume
36pub_type
杂志文章abstract::The aim of the present study was to investigate biochemical and morphological changes in rat lenses following long-term UV B irradiation. After an irradiation period of 156 days with follow-up documentation by means of Scheimpflug photography, section-related biochemical analyses of the lenses as well as histological ...
journal_title:Ophthalmic research
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更新日期:1996-01-01 00:00:00
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更新日期:2020-10-07 00:00:00
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更新日期:2007-01-01 00:00:00
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