Abstract:
OBJECTIVE:In this study, we used a rat model of retinal detachment (RD) to investigate the effects of transient receptor potential mucolipin 1 (TRPML1) on photoreceptor cells and the underlying mechanism. METHODS:An RD model was established by subretinal injection of sodium hyaluronate, and mucolipin synthetic agonist 1 (ML-SA1) and dimethyl sulfoxide (DMSO) were subretinally injected after RD induction. Retinal morphology was observed using haematoxylin-eosin (HE) staining, and the apoptosis of photoreceptor cells was detected by transmission electron microscopy (TEM). Reactive oxygen species (ROS) were examined with a ROS detection kit. The retinal expression levels of TRPML1, the autophagy-related protein microtubule-associated protein 1 light chain 3 (LC3), Beclin1, and cleaved caspase3 were detected by western blotting. The Morris water maze was used to test vision-dependent behaviour. RESULTS:We found that retinal structure and the outer nuclear layer (ONL) were improved and that the apoptosis of photoreceptor cells was reduced after ML-SA1 injection. The expression of ROS was reduced, and the loss of TRPML1 was inhibited after ML-SA1 treatment. The LC3-II to LC3-I ratio and Beclin1 expression were enhanced, and cleaved caspase3 expression was decreased after ML-SA1 treatment. Treatment with ML-SA1 also improved vision-dependent behaviour. CONCLUSIONS:Our findings suggest that ML-SA1 attenuates photoreceptor apoptosis and improves vision-dependent behavior by activation of autophagy.
journal_name
Ophthalmic Resjournal_title
Ophthalmic researchauthors
Yan Y,Wang Y,Ding J,Lu L,Ke G,Dong Kdoi
10.1159/000512104subject
Has Abstractpub_date
2020-10-07 00:00:00eissn
0030-3747issn
1423-0259pii
000512104pub_type
杂志文章abstract::The tear levels of cholesterol of 17 patients with chronic blepharitis and 17 healthy subjects were determined by the photometric method. The mean cholesterol level of the patients was determined to be 56.1 +/- 8.9 (SEM) mg/dl. No statistically significant difference between the tear cholesterol levels of the two grou...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267034
更新日期:1990-01-01 00:00:00
abstract::Cannabinoid receptor involvement in the intraocular-pressure (IOP)-lowering effect of the cannabinoids has not been determined. These receptors bind four structural classes of agonists: the classical and the nonclassical cannabinoids, the aminoalkylindoles and the arachidonylethanolamides (the endogenous ligands). We ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267984
更新日期:1997-01-01 00:00:00
abstract::Small RNA (microRNA or miRNA) is a kind of small noncoding single-stranded RNA that regulates complementary mRNA at the posttranscriptional level in eukaryotic organisms. As important regulatory factors, miRNAs play an important role in the occurrence and development of glaucoma and widely participate in regulating bi...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000450957
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:To investigate whether different crush durations or a different fluorogold (FG) injection timing can affect the efficiency of FG retrograde labeling of retinal ganglion cells (RGCs) in the optic nerve (ON) crush model. METHODS:We performed the ON crush in rats with a clip at different durations or a jewel forc...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000357736
更新日期:2014-01-01 00:00:00
abstract::Local pO2 was measured close to the ciliary process of the rabbit eye using polarographic microelectrodes under microendoscopic observation. The experiments were performed in 5 anaesthetized New Zealand rabbits. Changes in local pO2, intraocular pressure (IOP) and blood flow velocity (BFV) were investigated after intr...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267324
更新日期:1993-01-01 00:00:00
abstract:PURPOSE:To investigate the role of integrin-linked kinase (ILK) in endothelial cell migration, proliferation and tube formation in vitro. MATERIALS AND METHODS:Cultured RF/6A cells were knocked down for ILK using small interfering RNA (siRNA). Cellular ILK expression was quantified by real-time quantitative PCR and We...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000232971
更新日期:2009-01-01 00:00:00
abstract::The eyes of 200 rats (Mol:SPRD, Moellegaard Ltd., Skensvet, Denmark), 8 weeks of age and of both sexes, were examined routinely with a photo-slitlamp microscope (Zeiss) and opthalmoscope (Heine) 3-4 times in the course of four different 12-week toxicity studies. The animals were kept under constant lighting conditions...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267492
更新日期:1994-01-01 00:00:00
abstract:INTRODUCTION:Retinitis pigmentosa (RP) is an inherited retinal disorder, characterized by photoreceptor degeneration inducing progressive vision loss. This study evaluates its impact on quality of life (QOL) and emotional states of patients affected by RP. METHODS:A cross-sectional study was conducted on 60 RP patient...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000435886
更新日期:2015-01-01 00:00:00
abstract::Adult human lenses with focal opacities were processed for normal as well as freeze fracture electron microscopy. Cholesterol was demonstrated using filipin cytochemistry. Filipin cytochemistry in combination with freeze fracture revealed that the amount of cholesterol in the normal fibre membranes was fully comparabl...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000055627
更新日期:2000-11-01 00:00:00
abstract::Previous research from this laboratory showed that human lacrimal fluid peroxidase has cyclic variations during the menstrual cycle, correlated with plasma levels of 17beta-oestradiol. In the present investigation, variations of enzyme activity and total protein content during the oestrous cycle of young adult female ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000055666
更新日期:2001-05-01 00:00:00
abstract:AIMS:Experimental retinal research has gained great importance due to the ophthalmic pharmacotherapy era. An increasing number of drugs are constantly released into the market for the treatment of retinal diseases. In this review, animal species, animal models and toxicity assays in retinal research are discussed. MET...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000312817
更新日期:2010-01-01 00:00:00
abstract::A method for microscopical observation and quantification of corneal neovascularization in the living mouse is described. Measurements of the corneal vasculature were made on still-mode video-pictures of the cornea. The validity of the method was shown by the high correlation between the morphometric measurements and ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267097
更新日期:1991-01-01 00:00:00
abstract:PURPOSE:To propose a new noncontact tonometer based on corneal photoelasticity. METHOD:In this study, we experimented with 18 enucleated porcine eyes. The anterior chamber was infused with a physiological solution. A circular polarizing filter was attached to an ophthalmic surgical microscope. Color fringe changes at ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000320600
更新日期:2011-01-01 00:00:00
abstract::In order to know the correlation of a drug with high affinity to melanin in the iris ciliary body with the amount of melanin contained in the iris ciliary tissues, an in vivo study was performed on pigmented rabbits with brown or blue irises and albino rabbits. The melanin concentrations in the iris ciliary bodies of ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267404
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:The aim of this study is to provide a quantitative evaluation of the blood-aqueous barrier (BAB) in patients with ocular blunt trauma and evaluate its association with intraocular pressure (IOP) elevation. METHODS:This is a prospective case-control study, and the following 3 groups were included: elevated I...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000445352
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVE:Transient receptor potential canonical (TRPC) channels are involved in neovascularization repairing after vascular injury in many tissues. However, whether TRPCs play a regulatory role in the development of diabetic retinopathy (DR) has rarely been reported. In the present study, we selected TRPC1, 3, and 6 t...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000503724
更新日期:2020-01-01 00:00:00
abstract:PURPOSE:To compare tear film osmolarity (TFO) between patients with diabetes mellitus (DM) and normal healthy individuals. METHODS:In this prospective case-controlled study, the TFO in 46 normal subjects (control group) and 55 patients with DM (study group) was evaluated. TFO in milliosmole (mOsm) was measured by usin...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000345770
更新日期:2013-01-01 00:00:00
abstract::VEGF is considered as an important factor in the pathogenesis of macular edema. VEGF induces the rupture of the blood retinal barrier and may also influence the retinal pigment epithelial (RPE) outer retinal barrier. The aim of this work was to analyze the influence of the VEGF receptor pathways in the modulation of t...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000119877
更新日期:2008-01-01 00:00:00
abstract::The influence of nonselective and selective alpha-adrenergic agonists on intraocular pressure (IOP) was studied in isolated arterially perfused rabbit eyes. Drugs were given intra-arterially or topically. With either route of administration, no initial hypertensive phase was observed after l-epinephrine, l-norepinephr...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265400
更新日期:1985-01-01 00:00:00
abstract::Vitamin E (d-alpha-tocopherol) and aspirin were evaluated for their effects on the phase separation temperature, Tc, of calf lens cytoplasm. Vitamin E and aspirin were added to nuclear homogenate and the change in Tc was measured. Aspirin had no measurable effect on Tc. Vitamin E lowered Tc at a rate of -22 degrees C ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265475
更新日期:1987-01-01 00:00:00
abstract::The polyamines putrescine, spermidine and spermine have been proposed to be a part of the acute phase inflammatory response. They have been shown to be useful markers for cellular kinetics and change with various pathological conditions. The hypothesis that aqueous humor polyamines could be used to follow the time cou...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267165
更新日期:1992-01-01 00:00:00
abstract:PURPOSE:To evaluate the role of initial visual acuity (VA) as a potential prognostic factor for final VA in patients with branch retinal vein occlusion (BRVO). METHODS:A retrospective data analysis involving 163 patients with macular edema secondary to BRVO treated according to the recommendations of the Branch Vein O...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000321701
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:To investigate the association between retinal neovascularization and the CC chemokine receptor-3 (CCR3) in a mouse model of oxygen-induced retinopathy (OIR). METHODS:An OIR model in C57BL/6J mice was used as a retinal neovascularization model. An enzyme-linked immunosorbent assay was performed to evaluate the...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000463238
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:Single-field non-mydriatic fundus photography (NMFP) has been used to detect diabetic retinopathy (DR) in many studies; however, its value in a general clinical setting has not been established. Here we performed a meta-analysis to evaluate its diagnostic effectiveness. METHOD:We systematically searched PubMed...
journal_title:Ophthalmic research
pub_type: meta分析
doi:10.1159/000499106
更新日期:2019-01-01 00:00:00
abstract::Although it is essential that intraocular pressure (IOP) be reduced in glaucoma treatment, it is also vitally important to provide sufficient blood flow to eye tissues so that healthy visual field is maintained. It is possible for an agent to reduce IOP and blood supply to the eye. In that case, glaucoma appears to be...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265445
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND:The aim of this work was to compare the efficacy of intravitreal dexamethasone implant (Ozurdex) and intravitreal ranibizumab (Lucentis) in the treatment of macular edema (ME) caused by retinal vein occlusion (RVO). METHODS:Thirty-two ME cases treated with Ozurdex and 32 ME cases treated with ranibizumab we...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000458534
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:The aim of the present study was to characterize the development of after-cataract in the rabbit by measuring its wet weight, protein, DNA and glycosaminoglycan (GAG) contents and using Scheimpflug and slitlamp analysis. Further, aqueous humor (AqH) leukocytes, protein and lens epithelial cell proliferation act...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000048320
更新日期:2002-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the feasibility of a new technique for the implantation of ultrathin substrates containing stem cell-derived retinal pigment epithelium (RPE) cells into the subretinal space of retina-degenerate Royal College of Surgeon (RCS) rats. METHODS:A platform device was used for the implantation of 4-µm-t...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000338749
更新日期:2012-01-01 00:00:00
abstract:PURPOSE:To analyze a multiflash multifocal electroretinogram in 20 patients with open-angle glaucoma (OAG). METHODS:The stimulation sequence consisted of a binary m-sequence (L(max) 200 cd/m(2), L(min) <1 cd/m(2)). Each m-sequence stimulus was followed by three global flashes (luminance: 400 cd/m(2)) at an interval of...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000048333
更新日期:2002-03-01 00:00:00
abstract:INTRODUCTION:Amblyopia is an important public health problem and standard screening is quite necessary for early diagnosis and treatment especially for the remote areas. As the place of the largest Tibetan population, the Tibetan Plateau has special geographical characteristics such as high altitude, time zone, and eth...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000511264
更新日期:2020-12-04 00:00:00