Abstract:
PURPOSE:To investigate whether different crush durations or a different fluorogold (FG) injection timing can affect the efficiency of FG retrograde labeling of retinal ganglion cells (RGCs) in the optic nerve (ON) crush model. METHODS:We performed the ON crush in rats with a clip at different durations or a jewel forceps to compare the effects of different crush methods with FG staining. RGC density was compared between the FG injection 1 week before the sacrifice of the animals (group A) and the injection before the crush experiment (group B). Double staining with CD11b and FG in the retinal sections was conducted to investigate the relationship between the overcounting of RGCs and microglia. RESULTS:The FG-stained particles were significantly decreased at the distal part of the crush site compared to the proximal site of the ON with a crush duration of over 30 s or when crushed with the jewel forceps. Two weeks after ON crush, the RGC count was higher both in the central and mid-peripheral retinas in group B. The percentage of CD11b-stained cells among the FG-stained cells in the RGC layer of retinas in group B was higher than that of group A (34% in group B vs. 4% in group A, p = 0.0001). Overcounting of RGC density in group B was due to additional microglia with FG engulfing. CONCLUSIONS:Our results suggest that each laboratory should test its setting conditions to avoid factors influencing the RGC density measurement before conducting ON crush experiments.
journal_name
Ophthalmic Resjournal_title
Ophthalmic researchauthors
Huang TL,Huang SP,Chang CH,Lin KH,Sheu MM,Tsai RKdoi
10.1159/000357736subject
Has Abstractpub_date
2014-01-01 00:00:00pages
173-8issue
4eissn
0030-3747issn
1423-0259pii
000357736journal_volume
51pub_type
杂志文章abstract::The high cytotoxicity of linoleic acid (LA) to cultured bovine lens epithelial cells is correlated with high uptake rates for the fatty acid (FA). Both, LA uptake and LA cytotoxicity strongly increase with the increasing LA-to-albumin molar ratio in the culture medium. Cellular uptake and cytotoxicity of LA can be com...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000055595
更新日期:2000-03-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
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abstract:INTRODUCTION:Retinitis pigmentosa (RP) is an inherited retinal disorder, characterized by photoreceptor degeneration inducing progressive vision loss. This study evaluates its impact on quality of life (QOL) and emotional states of patients affected by RP. METHODS:A cross-sectional study was conducted on 60 RP patient...
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journal_title:Ophthalmic research
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doi:10.1159/000321701
更新日期:2011-01-01 00:00:00
abstract:AIMS:To investigate whether allogeneic limbal mesenchymal stem cell (LMSC) therapy affects corneal healing after a severe chemical burn and whether the route of administration of LMSCs differs in its therapeutic effect in this respect. METHODS:A total of 60 Sprague-Dawley rats with clinically proven alkali injury were...
journal_title:Ophthalmic research
pub_type: 杂志文章
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abstract::Using cuprolinic blue staining, we histochemically examined the ultrastructural localization of proteoglycans in the fibrous-type human posterior capsule opacification. This capsule opacification contained proteoglycans that showed positive staining with cuprolinic blue. Many cuprolinic-blue-positive filaments were pr...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267707
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abstract::The aqueous humors of 100 freshly enucleated rabbit eyes were assessed within 10 min postmortem by taking samples in the last stage of corneal dissection following the Dikstein-Maurice procedure. Aqueous levels of partial pressure of carbon dioxide (pCO2) averaged 58.2 +/- 8.5 mm Hg to give calculated tCO2 and bicarbo...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267269
更新日期:1993-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000321952
更新日期:2011-01-01 00:00:00
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journal_title:Ophthalmic research
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journal_title:Ophthalmic research
pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000272021
更新日期:2010-01-01 00:00:00
abstract::During the process of terminal differentiation, the fibre cells in the eye lens undergo many changes that are reminiscent of apoptotic/necrotic changes. Mitochondria, for instance, undergo permeability transition and nuclear degradation is accompanied by chromatin condensation, disintegration of the nucleolus, dissolu...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
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更新日期:1999-01-01 00:00:00
abstract::Rat lenses with experimentally induced cataract (either by naphthalene or by streptozotocin) were analyzed biochemically. Both noxae had some effects in common. Water-soluble protein and aldose reductase activity decreased, and glucose-6-phosphate dehydrogenase, phosphofructokinase and glutathione reductase activity i...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000266581
更新日期:1988-01-01 00:00:00
abstract::Results of a comparative study of accommodative capability of eyes in individuals exposed to various doses of ionizing radiation and in a control group are presented. The mean values for ranges of accommodation in the control group were consonant with previous data. However, the range of accommodation was found to be ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000063879
更新日期:2002-07-01 00:00:00
abstract::In corneal organ culture, a contamination of sterile culture media with endotoxin is frequently found. Thus, we investigated if the presence of endotoxin affects the viability of cultured porcine corneal endothelial cells. Endotoxin in high concentrations caused morphological cell changes in porcine corneal endothelia...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000055566
更新日期:1999-01-01 00:00:00
abstract:PURPOSE:To compare tear film osmolarity (TFO) between patients with diabetes mellitus (DM) and normal healthy individuals. METHODS:In this prospective case-controlled study, the TFO in 46 normal subjects (control group) and 55 patients with DM (study group) was evaluated. TFO in milliosmole (mOsm) was measured by usin...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000345770
更新日期:2013-01-01 00:00:00
abstract::This study is a first approach to identify UVA-related alterations in situ of bovine eye lens proteins from the water-soluble and urea-soluble fractions upon aging. The fractions were obtained from irradiated long-term organ culture lenses and analyzed by mini two-dimensional gel electrophoresis. This micropreparative...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000055613
更新日期:2000-09-01 00:00:00
abstract:PURPOSE:To evaluate the effect of transpupillary thermotherapy (TTT) on foveal thickness and macular function in eyes with choroidal neovascularization (CNV) associated with age-related macular degeneration. METHODS:Sixteen eyes with occult CNV and 6 eyes with classic CNV were treated with TTT. Optical coherence tomog...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000083020
更新日期:2005-01-01 00:00:00
abstract::The development and extension of fibrovascular or fibroglial membranes onto the retinal surface are a major cause of visual loss in diabetic patients with proliferative retinopathy and in patients suffering from retinal detachment with proliferative vitreoretinopathy. The pathogenesis of these proliferative diseases, ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267115
更新日期:1991-01-01 00:00:00
abstract:AIM:The aim of the current study is to evaluate the role of povidone iodine (PVI) in the management of experimental bacterial endophthalmitis in young rabbits. METHOD:Twenty white rabbits were used. Colony-forming units (CFU) of Staphylococcus epidermidis were injected intravitreally into the right eye of each animal....
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000217721
更新日期:2009-01-01 00:00:00
abstract:PURPOSE:The aim of the present study was to characterize the development of after-cataract in the rabbit by measuring its wet weight, protein, DNA and glycosaminoglycan (GAG) contents and using Scheimpflug and slitlamp analysis. Further, aqueous humor (AqH) leukocytes, protein and lens epithelial cell proliferation act...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000048320
更新日期:2002-01-01 00:00:00
abstract::Ibudilast selectively vasodilates cerebral vessels without reducing blood pressure. We investigated the effect of the drug on retinal circulation in 8 patients with diabetes mellitus, using the video-densitometric image analysis of fluorescein angiography. We compared the build up time, the time constant of washout ra...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267168
更新日期:1992-01-01 00:00:00
abstract::A system for isolation of capillaries from the retina has been adapted to biochemical studies in vitro. Insulin receptors were identified in human and calf retinal blood vessels. Binding of 125I insulin by calf retinal blood vessels takes place through high affinity (low capacity) and low affinity (high capacity) rece...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265234
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the feasibility of a new technique for the implantation of ultrathin substrates containing stem cell-derived retinal pigment epithelium (RPE) cells into the subretinal space of retina-degenerate Royal College of Surgeon (RCS) rats. METHODS:A platform device was used for the implantation of 4-µm-t...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000338749
更新日期:2012-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000507963
更新日期:2020-04-20 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000055545
更新日期:1999-01-01 00:00:00
abstract::Photodocumented measurements of the lens thickness using an EAS-1000 Scheimpflug camera were performed in 261 cataract eyes (162 pure type) compared with 52 control eyes. The lens thickness in normal eyes usually increased with age by 0.015 mm annually. The cataractous lens, especially in the anterior subcapsular type...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267205
更新日期:1992-01-01 00:00:00
abstract:PURPOSE:To characterize the 1-year progression of retinal thickness (RT) increase occurring in eyes with subclinical macular edema in type 2 diabetes. METHODS:Forty-eight type 2 diabetic eyes/patients with mild nonproliferative diabetic retinopathy (NPDR; levels 20 and 35 in the Early Treatment Diabetic Retinopathy St...
journal_title:Ophthalmic research
pub_type: 杂志文章,多中心研究
doi:10.1159/000438793
更新日期:2015-01-01 00:00:00
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journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265197
更新日期:1982-01-01 00:00:00