Abstract:
:Vaccines preventing HIV-1 infection will likely elicit antibodies that neutralize diverse strains. However, the capacity for lentiviruses to escape broadly neutralizing antibodies (NAbs) is not completely understood, nor is it known whether NAbs alone can control heterologous infection. Here, we determined that convalescent immune plasma from a horse persistently infected with equine infectious anemia virus (EIAV) neutralized homologous virus and several envelope variants containing heterologous principal neutralizing domains (PND). Plasma was infused into young horses (foals) affected with severe combined immunodeficiency (SCID), followed by challenge with a homologous EIAV stock. Treated SCID foals were protected against clinical disease, with complete prevention of infection occurring in one foal. In three SCID foals, a novel neutralization-resistant variant arose that was found to preexist at a low frequency in the challenge inoculum. In contrast, SCID foals infused with nonimmune plasma developed acute disease associated with high levels of the predominant challenge virus. Following transfer to an immunocompetent horse, the neutralization-resistant variant induced a single febrile episode and was subsequently controlled in the absence of type-specific NAb. Long-term control was associated with the presence of cytotoxic T lymphocytes (CTL). Our results demonstrate that immune plasma with neutralizing activity against heterologous PND variants can prevent lentivirus infection and clinical disease in the complete absence of T cells. Importantly, however, rare neutralization-resistant envelope variants can replicate in vivo under relatively broad selection pressure, highlighting the need for protective lentivirus vaccines to elicit NAb responses with increased breadth and potency and/or CTL that target conserved epitopes.
journal_name
J Viroljournal_title
Journal of virologyauthors
Taylor SD,Leib SR,Carpenter S,Mealey RHdoi
10.1128/JVI.00218-10subject
Has Abstractpub_date
2010-07-01 00:00:00pages
6536-48issue
13eissn
0022-538Xissn
1098-5514pii
JVI.00218-10journal_volume
84pub_type
杂志文章abstract::Natural killer (NK) cells derived from the human female reproductive tract (FRT) are phenotypically and functionally distinct from those obtained from peripheral blood. Because the FRT is a primary site of human immunodeficiency virus type 1 (HIV-1) infection in women, we determined whether soluble factors secreted by...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
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doi:10.1128/JVI.27.1.193-204.1978
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pub_type: 杂志文章
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journal_title:Journal of virology
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doi:10.1128/JVI.31.1.1-7.1979
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pub_type: 杂志文章
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journal_title:Journal of virology
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doi:10.1128/JVI.9.4.698-700.1972
更新日期:1972-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.65.5.2290-2298.1991
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.21.10550-10556.2001
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11710-11714.2002
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5376-5382.1990
更新日期:1990-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.7.3624-3633.2006
更新日期:2006-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.2739-2744.1999
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3930-3937.1996
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.56.3.1023-1026.1985
更新日期:1985-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.12.5974-5984.2002
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.3.686-691.1984
更新日期:1984-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.10.5414-5422.2004
更新日期:2004-05-01 00:00:00