Abstract:
:The human T-cell leukemia virus type 1 (HTLV-1) transmembrane glycoprotein has a 24-amino-acid cytoplasmic domain whose function in the viral life cycle is poorly understood. We introduced premature-stop mutations and 18 single-amino-acid substitutions into this domain and studied their effects on cell-to-cell transmission of the virus. The results show that the cytoplasmic domain is absolutely required for cell-to-cell transmission of HTLV-1, through amino acids which cluster in a Y-S-L-I tyrosine-based motif. The transmission defect in two motif mutants did not result from a defect in glycoprotein incorporation or fusion. It appears that the Y-S-L-I tyrosine-based motif of the HTLV-1 glycoprotein cytoplasmic domain has multiple functions, including involvement in virus transmission at a postfusion step.
journal_name
J Viroljournal_title
Journal of virologyauthors
Delamarre L,Pique C,Rosenberg AR,Blot V,Grange MP,Le Blanc I,Dokhélar MCdoi
10.1128/JVI.73.11.9659-9663.1999subject
Has Abstractpub_date
1999-11-01 00:00:00pages
9659-63issue
11eissn
0022-538Xissn
1098-5514journal_volume
73pub_type
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journal_title:Journal of virology
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journal_title:Journal of virology
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