Analysis of wt1a reporter line expression levels during proepicardium formation in the zebrafish.

Abstract:

:The epicardium is the outer mesothelial layer of the heart. It covers the myocardium and plays important roles in both heart development and regeneration. It is derived from the proepicardium (PE), groups of cells that emerges at early developmental stages from the dorsal pericardial layer (DP) close to the atrio-ventricular canal and the venous pole of the heart-tube. In zebrafish, PE cells extrude apically into the pericardial cavity as a consequence of DP tissue constriction, a process that is dependent on Bmp pathway signaling. Expression of the transcription factor Wilms tumor-1, Wt1, which is a leader of important morphogenetic events such as apoptosis regulation or epithelial-mesenchymal cell transition, is also necessary during PE formation. In this study, we used the zebrafish model to compare intensity level of the wt1a reporter line epi:GFP in PE and its original tissue, the DP. We found that GFP is present at higher intensity level in the PE tissue, and differentially wt1 expression at pericardial tissues could be involved in the PE formation process. Our results reveal that bmp2b overexpression leads to enhanced GFP level both in DP and in PE tissues.

journal_name

Histol Histopathol

authors

Andrés-Delgado L,Galardi-Castilla M,Mercader N,Santamaría L

doi

10.14670/HH-18-238

subject

Has Abstract

pub_date

2020-09-01 00:00:00

pages

1035-1046

issue

9

eissn

0213-3911

issn

1699-5848

pii

HH-18-238

journal_volume

35

pub_type

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