Liver growth factor antifibrotic activity in vivo is associated with a decrease in activation of hepatic stellate cells.

Abstract:

:The antifibrotic activity of Liver Growth Factor (LGF), a liver mitogen, was previously demonstrated in several models of rat liver fibrosis and even in extrahepatic sites, such as carotid artery in hypertensive rats and rat CdCl2-induced lung fibrosis. In the present study, we have attempted to examine in depth its mechanism of antifibrotic action in bile duct-ligated (BDL) rats, with special emphasis on its activity in fibrogenic liver cells. BDL rats received either LGF 9 microg/week for 2 or 3 weeks (BDL+LGF, n=20/group) or saline (BDL+S, n=20/group), at times 0, week 2, or week 5 after operation. Groups were compared in terms of liver alpha-smooth muscle actin (SMA) content (western blotting and immunohistochemistry), hepatic apoptosis, liver desmin content (western blotting), and serum endothelin-1 (ELISA). LGF produced a marked decrease in liver alpha-SMA content compared with saline-injected rats, especially evident at longer times (5w and 8w; p<0.05). Moreover, LGF did not seem to influence HSC proliferation, as shown by measuring liver desmin content. The antifibrotic activity exerted by LGF seems to be closely related to a modulation of the activation state of fibrogenic liver cells (activated HSC and myofibroblasts) in BDL rats.

journal_name

Histol Histopathol

authors

Díaz-Gil JJ,García-Monzón C,Rúa C,Martín-Sanz P,Cereceda RM,Miquilena-Colina ME,Machín C,Fernández-Martínez A,García-Cañero R

doi

10.14670/HH-24.473

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

473-9

issue

4

eissn

0213-3911

issn

1699-5848

journal_volume

24

pub_type

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