Liver growth factor antifibrotic activity in vivo is associated with a decrease in activation of hepatic stellate cells.


:The antifibrotic activity of Liver Growth Factor (LGF), a liver mitogen, was previously demonstrated in several models of rat liver fibrosis and even in extrahepatic sites, such as carotid artery in hypertensive rats and rat CdCl2-induced lung fibrosis. In the present study, we have attempted to examine in depth its mechanism of antifibrotic action in bile duct-ligated (BDL) rats, with special emphasis on its activity in fibrogenic liver cells. BDL rats received either LGF 9 microg/week for 2 or 3 weeks (BDL+LGF, n=20/group) or saline (BDL+S, n=20/group), at times 0, week 2, or week 5 after operation. Groups were compared in terms of liver alpha-smooth muscle actin (SMA) content (western blotting and immunohistochemistry), hepatic apoptosis, liver desmin content (western blotting), and serum endothelin-1 (ELISA). LGF produced a marked decrease in liver alpha-SMA content compared with saline-injected rats, especially evident at longer times (5w and 8w; p<0.05). Moreover, LGF did not seem to influence HSC proliferation, as shown by measuring liver desmin content. The antifibrotic activity exerted by LGF seems to be closely related to a modulation of the activation state of fibrogenic liver cells (activated HSC and myofibroblasts) in BDL rats.


Histol Histopathol


Díaz-Gil JJ,García-Monzón C,Rúa C,Martín-Sanz P,Cereceda RM,Miquilena-Colina ME,Machín C,Fernández-Martínez A,García-Cañero R




Has Abstract


2009-04-01 00:00:00












  • Classification of routinely processed anaplastic large cell tumours with a small panel of antibodies. An immunohistochemical study with clinical follow-up.

    abstract::A proportion of anaplastic large cell tumours is difficult to classify on sections of routinely processed, paraffin-embedded tissue. Differentiation into large cell lymphoma, carcinoma, melanoma or sarcoma is important in order to assess prognosis and proper treatment. Although the use of immunohistochemistry has been...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Henzen-Logmans SC,Mullink H,Vennegoor C,Hilgers J,Oort J,Meijer CJ

    更新日期:1987-04-01 00:00:00

  • Expression of cyclooxygenase-2 in invasive breast carcinomas and its prognostic impact.

    abstract::Representative tumour sections from 468 patients with invasive breast cancer were immunostained for cyclooxygenase-2 (COX-2) and evaluated. The relationships between COX-2 expression, clinical outcome and various clinicopathological variables, including tumour vascularity and disseminated tumour cells (DTC) in the bon...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Dhakal HP,Naume B,Synnestvedt M,Borgen E,Kaaresen R,Schlichting E,Wiedswang G,Bassarova A,Holm R,Giercksky KE,Nesland JM

    更新日期:2012-10-01 00:00:00

  • Nigral degeneration correlates with persistent activation of cerebellar Purkinje cells in MPTP-treated monkeys.

    abstract::In the present work we analyze the cerebellum of chronic parkinsonian monkeys in order to clarify whether chronic mesencephalic depletion is associated with long term activation of the cerebellar neurons in chronic Parkinsonism. In our study, we observed a persistent activation of Purkinje cells in the cerebellum of c...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Heman P,Barcia C,Gómez A,Ros CM,Ros-Bernal F,Yuste JE,de Pablos V,Fernandez-Villalba E,Toledo-Cárdenas MR,Herrero MT

    更新日期:2012-01-01 00:00:00

  • Immunohistochemistry of connexin43 and zonula occludens-1 in the myocardium as markers of early ischemia in autopsy material.

    abstract::Immunohistochemistry of the terminal complement complex (C5b-9) and fibronectin (FN) is useful to detect myocardial ischemia preceding necrosis in the postmortem diagnosis of sudden cardiac death. The present immunohistochemical study examined connexin43 (Cx43) and zonula occludens-1 (ZO-1) as markers of early myocard...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Kawamoto O,Michiue T,Ishikawa T,Maeda H

    更新日期:2014-06-01 00:00:00

  • Muscle-derived stem cells in tissue engineering: defining cell properties suitable for construct design.

    abstract::The terms construct or tissue equivalent refer to neotissue produced by tissue engineering techniques. The elements forming the construct are scaffolds on which cells are "recreated" to form an engineered-tissue sensitive to certain cell signals. The ability of the cells to expand and differentiate on the scaffold is ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Buján J,Pascual G,Corrales C,Gómez-Gil V,Rodríguez M,Bellón JM

    更新日期:2005-07-01 00:00:00

  • Prognostic significance of PTK7 in human malignancies.

    abstract:BACKGROUND:Protein tyrosine kinase 7 (PTK7) is a member of receptor protein tyrosine kinase-like molecules, which is involved in tumorigenesis. However, the association between PTK7 expression and its pathological significance in survival prognosis remains under investigation. The purpose of this meta-analysis is to cl...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,meta分析


    authors: Chen G,Qi S,Yang X,Chen W

    更新日期:2018-04-01 00:00:00

  • Modulatory role of IL10 in endothelial cell damage and platelet adhesion.

    abstract::This study explores the possibility of a regulatory role for cytokine IL-10 in platelet aggregation as an active vascular repair mechanism. Endothelial cells from human umbilical cord vein were cultured in the presence of different IL-10 concentrations (0-100 ng/ml). Platelet-rich plasma was then added to these cultur...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Gimeno MJ,Pascual G,García-Honduvilla N,Prieto A,Alvarez de Mon M,Bellón JM,Buján J

    更新日期:2003-07-01 00:00:00

  • Anti-platelet agents reduce morphological changes of chronic hypoxic pulmonary hypertension.

    abstract::The pathophysiologic mechanism by which chronic hypoxia causes pulmonary hypertension is unknown. If anti-platelet agents, or other pharmacologic interventions, altered the pulmonary vascular changes induced by hypoxia, information concerning the pathogenesis of the pulmonary hypertension or the potential therapeutic ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Keith IM,Will JA,Huxtable RJ,Weir K

    更新日期:1987-04-01 00:00:00

  • Apoptosis and autophagy in nigral neurons of patients with Parkinson's disease.

    abstract::Parkinson's disease (PD) is a neurodegenerative disorder characterized by progressive cell loss confined mostly to dopaminergic neurons of the substantia nigra. Several factors, including oxidative stress, and decreased activity of complex I mitochondrial respiratory chain, are involved in the degenerative process. Ye...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Anglade P,Vyas S,Javoy-Agid F,Herrero MT,Michel PP,Marquez J,Mouatt-Prigent A,Ruberg M,Hirsch EC,Agid Y

    更新日期:1997-01-01 00:00:00

  • Pathological changes of human unmyelinated nerve fibers: a review.

    abstract::In the cutaneous nerves, unmyelinated nerve fibers outnumber the myelinated ones but are scarcely analyzed, especially at autopsy. This indifference toward the pathology of unmyelinated nerve fibers may be due to the necessity of electron microscopic analyses and, more importantly, the obscurity of pathological altera...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审


    authors: Kanda T

    更新日期:2000-01-01 00:00:00

  • The deep-sea teleost cornea: a comparative study of gadiform fishes.

    abstract::The corneal structure of three deep-sea species of teleosts (Gadiformes, Teleostei) from different depths (250-4000 m) and photic zones are examined at the level of the light and electron microscopes. Each species shows a similar but complex arrangement of layers with a cornea split into dermal and scleral components....

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Collin SP,Collin HB

    更新日期:1998-04-01 00:00:00

  • Commonly used mesenchymal stem cell markers and tracking labels: Limitations and challenges.

    abstract::Early observations that cultured mesenchymal stem cells (MSCs) could be induced to exhibit certain characteristics of osteocytes and chondrocytes led to the proposal that they could be transplanted for tissue repair through cellular differentiation. Therefore, many subsequent preclinical studies with transplanted MSCs...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审


    authors: Lin CS,Xin ZC,Dai J,Lue TF

    更新日期:2013-09-01 00:00:00

  • Markers of squamocolumnar junction cells in normal tonsils and oropharyngeal cancer with and without HPV infection.

    abstract::HPV infection has been identified recently as the causative agent of a subset of squamous cell carcinomas arising in oropharyngeal tonsils. Factors influencing the susceptibility of tonsillar epithelium to HPV-induced oncogenesis are far from being elucidated. A 5-protein signature including cytokeratin (CK)7, anterio...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Morbini P,Capello GL,Alberizzi P,Benazzo M,Paglino C,Comoli P,Pedrazzoli P

    更新日期:2015-07-01 00:00:00

  • Estimation and comparison of the contents of blood group B antigens in selected human tissues by microphotometric quantification of Griffonia simplicifolia agglutinin I-B4 staining with or without prior alpha-galactosidase digestion.

    abstract::Griffonia simplicifolia agglutinin I-B4 (GSAI-B4) has broader specificity for B antigen variants and can recognize the antigens in a wide variety of human tissues. Thus, the concentration range of GSAI-B4 required for staining and the susceptibility of staining to alpha-galactosidase digestion is presumed to correlate...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Ito N,Nagaike C,Morimura Y,Hatake H

    更新日期:1997-04-01 00:00:00

  • Bone marrow histopathology in chronic myelogenous leukemia (CML)--evaluation of distinctive features with clinical impact.

    abstract::Bone marrow features in stable-phase chronic myelogenous leukemia (CML) are characterized by a striking heterogeneity which is determinable by appropriate means including representative pre-treatment trephine biopsies, immunohistochemistry and morphometry. Cell lineages involved to a variable extent consist not only o...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审


    authors: Thiele J,Kvasnicka HM,Fischer R

    更新日期:1999-10-01 00:00:00

  • In vivo cellular uptake of bismuth ions from shotgun pellets.

    abstract::Shotgun pellets containing bismuth (Bi) are widely used and may cause a rather intense exposure of some wild animals to Bi. A Bi shotgun pellet was implanted intramuscularly in the triceps surae muscle of 18 adult male Wistar rats. Another group of 9 animals had a Bi shotgun pellet implanted intracranially in the neoc...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Stoltenberg M,Locht L,Larsen A,Jensen D,Danscher G

    更新日期:2003-07-01 00:00:00

  • Effects of thyroid hormones on Leydig cells in the postnatal testis.

    abstract::Thyroid hormones (TH) stimulate oxidative metabolism in many tissues in the body, but testis is not one of them. Therefore, in this sense, testis is not considered as a target organ for TH. However, recent findings clearly show that TH have significant functions on the testis in general, and Leydig cells in particular...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审


    authors: Mendis-Handagama SM,Ariyaratne HB

    更新日期:2004-07-01 00:00:00

  • Morphological and functional changes in experimental ocular hypertension and role of neuroprotective drugs.

    abstract::Glaucoma is a neurodegenerative disease characterized by progressive loss of retinal ganglion cell axons and their cell bodies in the retina. Elevated intraocular pressure (IOP) is considered to be the major risk factor associated with the development of this neuropathy. Randomized controlled clinical trials have demo...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审


    authors: García-Campos J,Villena A,Díaz F,Vidal L,Moreno M,Pérez de Vargas I

    更新日期:2007-12-01 00:00:00

  • Sulfur dioxide: foe or friend for life?

    abstract::Sulfur dioxide (SO₂) is a toxic gas and air pollutant. The toxic effects of SO₂ have been extensively studied. Oxidative damage due to SO₂ can occur in multiple organs. Inhaled SO₂ can also cause chromosomal aberrations, DNA damage and gene mutations in mammals. However, SO₂ can also be generated from the sulfur-conta...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审


    authors: Wang XB,Cui H,Liu X,Du JB

    更新日期:2017-12-01 00:00:00

  • Overexpression of YES1 is associated with favorable prognosis and increased platinum-sensitivity in patients with epithelial ovarian cancer.

    abstract:AIMS:The prognostic application of YES1 in epithelial ovarian cancer (EOC) is currently unclear. We aimed to investigate the expression of YES1 and its correlation with survival outcome in patients with EOC. METHODS:A retrospective study of patients diagnosed with EOC at the Cancer Center, Sun Yat-Sen University, Guan...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Zhou Y,Chen P,Huang Q,Wan T,Jiang Y,Jiang S,Yan S,Zheng M

    更新日期:2020-07-01 00:00:00

  • Advances in translational orthopaedic research with species-specific multipotent mesenchymal stromal cells derived from the umbilical cord.

    abstract::Compliance with current regulations for the development of innovative medicines require the testing of candidate therapies in relevant translational animal models prior to human use. This poses a great challenge when the drug is composed of cells, not only because of the living nature of the active ingredient but also...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审


    authors: Ramallo M,Carreras-Sánchez I,López-Fernández A,Vélez R,Aguirre M,Feldman S,Vives J

    更新日期:2020-09-11 00:00:00

  • Localization of the calcium channel subunits Cav1.2 (alpha1C) and Cav2.3 (alpha1E) in the mouse organ of Corti.

    abstract::Voltage-activated Ca2+ channels play an important role in synaptic transmission, signal processing and development. The immunohistochemical localization of Cav1.2 (alpha1C) and Cav2.3 (alpha1E) Ca2+ channels was studied in the developing and adult mouse organ of Corti using subunit-specific antibodies and fluorescent ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Waka N,Knipper M,Engel J

    更新日期:2003-10-01 00:00:00

  • Histochemical study of skin and gills of Senegal sole, Solea senegalensis larvae and adults.

    abstract::A battery of horseradish peroxidase-conjugated lectins (Con A, WGA and DBA), as well as conventional histochemical techniques (PAS, saponification, Alcian Blue pH 0.1, 1, 2.5, chlorhydric hydrolisis, neuraminidase, Bromophenol blue, Tioglycollate reduction and Ferric-ferricyanide-FeIII) were used to study the content ...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Sarasquete C,González de Canales ML,Arellano J,Muñoz Cueto JA,Ribeiro L,Dinis MT

    更新日期:1998-07-01 00:00:00

  • Efficient uptake of mannosylated proteins by a human Schwann cell line.

    abstract::Complex carbohydrate structures are essential molecules of infectious microbes and host cells, and are involved in cell signaling associated with inflammatory and immune responses. The uptake of mannose-tailed glycans is usually carried out by macrophages, dendritic cells (DCs), and other professional phagocytes to tr...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Baetas-da-Cruz W,Alves L,Guimarães EV,Santos-Silva A,Pessolani MC,Barbosa HS,Corte-Real S,Cavalcante LA

    更新日期:2009-08-01 00:00:00

  • Abnormal distribution of CD45 isoforms expressed by CD4+ and CD8+ T cells in rheumatoid arthritis.

    abstract::CD45RO+ T cells are referred to as memory or helper-inducer while CD45RA+ T cells are regarded as naive or suppressor-inducer T cells. The former population predominates in the peripheral blood and even more in the synovial fluid of patients with rheumatoid arthritis, to the expense of the latter population. Within th...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审


    authors: Mamoune A,Durand V,Le Goff P,Pennec YL,Youinou P,Le Corre R

    更新日期:2000-04-01 00:00:00

  • Expression of the Shwachman-Bodian-Diamond syndrome (SBDS) protein in human pancreatic cancer and chronic pancreatitis.

    abstract:BACKGROUND:The Shwachman-Bodian-Diamond syndrome (SBDS) protein is a member of a highly conserved family which influences RNA activation and is associated with pancreatic, skeletal and bone marrow deficiencies, as well as hematological malignancies. METHODS:In this study, the expression and localization of SBDS were i...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Kayed H,Bekasi S,Keleg S,Welsch T,Esposito I,Shimamura A,Michalski CW,Friess H,Kleeff J

    更新日期:2008-07-01 00:00:00

  • Cisplatin treatment of NIH/3T3 cultures induces a form of autophagic death in polyploid cells.

    abstract::The effects induced by different concentrations (50, 75, 100 microM) of the cytostatic drug cisplatin (cDDP) in NIH/3T3 cells were analyzed. Sub-confluent cultures of this mouse fibroblast line, obtained after serum deprivation, showed the presence of aneuploid/polyploid cells with ploidy values ranging from 4c to 24c...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Spano A,Monaco G,Barni S,Sciola L

    更新日期:2008-06-01 00:00:00

  • Recombinant generation of two fragments of the rat complement inhibitory factor H [FH(SCR1-7) and FH(SCR1-4)] and their structural and functional characterization in comparison to FH isolated from rat serum.

    abstract::Factor H (FH) is the predominant soluble inhibitor of the complement system. With a concentration of 200-800 microg/ml in human and rat plasma it acts as a cofactor for the soluble factor I (FI)-mediated cleavage of the component C3b to iC3b. Furthermore it competes with factor B for binding to C3b and C3(H2O) and pro...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审


    authors: Demberg T,Heine I,Götze O,Altermann WW,Seliger B,Schlaf G

    更新日期:2006-01-01 00:00:00

  • Complex and elementary histological scoring systems for articular cartilage repair.

    abstract::The repair of articular cartilage defects is increasingly moving into the focus of experimental and clinical investigations. Histological analysis is the gold standard for a valid and objective evaluation of cartilaginous repair tissue and predominantly relies on the use of established scoring systems. In the past thr...

    journal_title:Histology and histopathology

    pub_type: 杂志文章,评审


    authors: Orth P,Madry H

    更新日期:2015-08-01 00:00:00

  • Comparative in vivo and in vitro models to approach the cellular basis of endotoxic shock. The role of sinusoidal liver cells.

    abstract::During endotoxic shock, the liver exerts a lipopolysaccharide (LPS) clearance function with the participation of both parenchymal and sinusoidal cells. Liver damage could be caused by LPS direct action, hypoxia and/or inflammatory mediators released by Kupffer cells. The aim of this study is to establish an experiment...

    journal_title:Histology and histopathology

    pub_type: 杂志文章


    authors: Pagani R,Portolés MT,Arahuetes R,Ainaga MJ,Machín C,Rua C

    更新日期:1996-07-01 00:00:00