Abstract：
:Bacillus subtilis has been used as a classic model to study biofilm formation and sporulation process. Colonies of wild-type strains usually have a complex external morphology, but the details of their internal architecture are still undisclosed. Since bacterial biofilms fulfill the criteria to be considered tissues, the aim of this work was to analyse B. subtilis colony-biofilm internal architecture evolution and sporulation dynamics using histological techniques. Transversal sections of colony-biofilms incubated from 24 hours up to 20 days were stained using histochemical techniques to analyse the internal structure by light and electron microscopy. A morphometric study of the different structural biofilm components was performed by image analysis, and an application to quantify spores was developed. Internal biofilm architecture was characterised by a stratified pattern, which evolved from 3 strata at 24 hours, up to 5 strata at 20 days. At 48 hours, strata at the central area of the biofilm was folded, resulting in elevated structures (vein-like structures) that could reach up to 465 μm in height. Sporulation started at 48 hours, at the top of the vein-like structures, at the interface between the two uppermost strata. At 20 days spores formed a continuous central layer, representing 7.5% of the total biofilm. In summary, our results demonstrate that B. subtilis colony-biofilm has a complex and organized internal architecture, evolving over time, and taking place in different cell subpopulations with different functionalities. Furthermore, in situ spore quantification described in this work could be a good alternative to the classical chamber counting.
journal_name
Histol Histopatholjournal_title
Histology and histopathologyauthors
Gómez-Aguado F,Corcuera MT,Gómez-Lus ML,de la Parte MA,Ramos C,García-Rey C,Alonso MJ,Prieto Jdoi
10.14670/HH-28.1351subject
Has Abstractpub_date
2013-10-01 00:00:00pages
1351-60issue
10eissn
0213-3911issn
1699-5848pii
HH-11-337journal_volume
28pub_type
杂志文章abstract：:This paper reports the histopathological findings in the eyes of a 26-year-old female patient diagnosed with systemic lupus erythematosus (SLE) with peripheral neuropathy. The patient had no significant ocular problems. She died of pneumonia after two years of suffering. The eyeballs were procured at autopsy and the r...
journal_title：Histology and histopathology
pub_type： 杂志文章
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更新日期：2005-04-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-30.173
更新日期：2015-02-01 00:00:00
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journal_title：Histology and histopathology
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更新日期：1992-10-01 00:00:00
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journal_title：Histology and histopathology
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更新日期：1986-10-01 00:00:00
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pub_type： 杂志文章,评审
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journal_title：Histology and histopathology
pub_type： 杂志文章,评审
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更新日期：2009-10-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
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journal_title：Histology and histopathology
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doi：10.14670/HH-13.1037
更新日期：1998-10-01 00:00:00
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journal_title：Histology and histopathology
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doi：10.14670/HH-14.805
更新日期：1999-07-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1997-10-01 00:00:00
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journal_title：Histology and histopathology
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doi：10.14670/HH-18-030
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-24.717
更新日期：2009-06-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-24.243
更新日期：2009-02-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-18.781
更新日期：2003-07-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-11-590
更新日期：2015-07-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-17.199
更新日期：2002-01-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-11-714
更新日期：2016-05-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-20.891
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journal_title：Histology and histopathology
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journal_title：Histology and histopathology
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-23.77
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journal_title：Histology and histopathology
pub_type： 杂志文章,评审
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更新日期：2013-06-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章,评审
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更新日期：1998-04-01 00:00:00
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journal_title：Histology and histopathology
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doi：10.14670/HH-27.807
更新日期：2012-06-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章,评审
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更新日期：2014-08-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章
doi：10.14670/HH-15.673
更新日期：2000-07-01 00:00:00
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journal_title：Histology and histopathology
pub_type： 杂志文章,评审
doi：10.14670/HH-15.983
更新日期：2000-07-01 00:00:00
