Prior exposure of agriculture cephalosporin ceftiofur impaired conjugation of blaCTX-M-65 gene-bearing plasmid in Salmonella Saintpaul.

Abstract:

AIMS:Although a link between agricultural cephalosporin use and resistance in Salmonella has been demonstrated with the drug ceftiofur, the underlying mechanism of the correlation is unclear. This study investigated the impact of ceftiofur exposure in S. Saintpaul on ceftriaxone resistance, the gene expression and the conjugative transfer of the blaCTX-M-65 gene. METHODS AND RESULTS:Prior ceftiofur exposure caused a twofold increase in MIC from 1024 to 2048 µg ml-1 towards ceftriaxone and increased the enzymatic activity of BlaCTX-M-65 2·2 folds from 3·46 to 7·67 nmol nitrocefin hydrolysed min-1 . A threefold upregulation in gene expression of the blaCTX-M-65 gene was also observed. Donors exposed to ceftiofur subsequently demonstrated a 2·5-fold decrease in transfer efficiency. CONCLUSIONS:Prior exposure of S. Saintpaul to ceftiofur led to increased phenotypic resistance towards ceftriaxone while its ability to spread the cephalosporin resistance through conjugation, conversely, was impaired. SIGNIFICANCE AND IMPACT OF THE STUDY:Findings from this study shed light on one possible mechanism in which agricultural cephalosporin exposure in Salmonella may subsequently impact clinical treatment. The finding that cephalosporin exposure in donors may hinder the subsequent spread of resistance instead of aiding it up was counter-intuitive.

journal_name

J Appl Microbiol

authors

Zwe YH,Yuk HG

doi

10.1111/jam.14749

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

1552-1565

issue

6

eissn

1364-5072

issn

1365-2672

journal_volume

129

pub_type

杂志文章
  • Escherichia coli cellular responses to exposure to atmospheric-pressure dielectric barrier discharge plasma-treated N-acetylcysteine solution.

    abstract:AIM:To understand the underlying cellular mechanisms during inactivation of Escherichia coli in response to antimicrobial solution of nonthermal plasma-activated N-acetylcysteine (NAC). METHODS AND RESULTS:The recommended techniques were used to demonstrate E. coli cellular and transcriptomic changes caused associated...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13777

    authors: Ercan UK,Sen B,Brooks AD,Joshi SG

    更新日期:2018-08-01 00:00:00

  • Immunogenicity of recombinant outer membrane porin protein and protective efficacy against lethal challenge with Bordetella bronchiseptica in rabbits.

    abstract:AIMS:The outer membrane porin protein (OMPP) of Bordetella bronchiseptica is an important adhesion factor and protective immunogen. The aim of this study was to verify the immunogenicity of recombinant OMPP and its protective efficacy against a lethal challenge with B. bronchiseptica in rabbits. METHODS AND RESULTS:So...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14451

    authors: Zhang H,Zhang H,Xiong B,Fan G,Cao Z

    更新日期:2019-12-01 00:00:00

  • Chrysin protects mice from Staphylococcus aureus pneumonia.

    abstract:AIM:To elucidate the effect of chrysin on α-haemolysin production by Staphylococcus aureus and protection against pneumonia in a murine model. METHODS AND RESULTS:Haemolysis, Western blot and real-time RT-PCR assays were performed to evaluate the effect of chrysin on α-haemolysin secretion by Staph. aureus. The effica...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05170.x

    authors: Wang J,Qiu J,Dong J,Li H,Luo M,Dai X,Zhang Y,Leng B,Niu X,Zhao S,Deng X

    更新日期:2011-12-01 00:00:00

  • Recent development and new insight of diversification and symbiosis specificity of legume rhizobia: mechanism and application.

    abstract::Currently, symbiotic rhizobia (sl., rhizobium) refer to the soil bacteria in α- and β-Proteobacteria that can induce root and/or stem nodules on some legumes and a few of nonlegumes. In the nodules, rhizobia convert the inert dinitrogen gas (N2 ) into ammonia (NH3 ) and supply them as nitrogen nutrient to the host pla...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.14960

    authors: Chen WF,Wang ET,Ji ZJ,Zhang JJ

    更新日期:2020-12-10 00:00:00

  • Dynamics of living and dead bacterial cells within a mixed-species biofilm during toluene degradation in a biotrickling filter.

    abstract:AIMS:To study the accumulation of the bacterial living cells (LC) and dead cells (DC) in a mixed-species biofilm developed in a 3 l biotrickling filter (BTF) challenged with toluene. METHODS AND RESULTS:The bacterial LC and DC within the biofilm developed on polypropylene Pall rings in a toluene-degrading BTF were enu...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01914.x

    authors: Tresse O,Lescob S,Rho D

    更新日期:2003-01-01 00:00:00

  • Flow cytometry: a versatile technology for specific quantification and viability assessment of micro-organisms in multistrain probiotic products.

    abstract:AIMS:Classical microbiology techniques are the gold standard for probiotic enumeration. However, these techniques are limited by parameters of time, specificity and incapacity to detect viable but nonculturable (VBNC) micro-organisms and nonviable cells. The aim of the study was to evaluate flow cytometry as a novel me...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13666

    authors: Chiron C,Tompkins TA,Burguière P

    更新日期:2018-02-01 00:00:00

  • Isolation and identification of Burkholderia pseudomallei from soil using selective culture techniques and the polymerase chain reaction.

    abstract::An environmental soil survey to detect Burkholderia pseudomallei was performed during the dry and wet seasons in Darwin, Northern Territory, Australia. Soil was sampled at regular intervals during a 15-month period at different depths from areas which were representative of the local, soil environment. Selective cultu...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:

    authors: Brook MD,Currie B,Desmarchelier PM

    更新日期:1997-05-01 00:00:00

  • Ecophysiological characterization of common food-borne fungi in relation to pH and water activity under various atmospheric compositions.

    abstract::The combined effects of pH, water activity (aw), oxygen (O2) and carbon dioxide (CO2) levels on growth and sporulation of 10 common food-borne fungi were studied. The use of a multivariate statistical method (PLS) for the analysis of data showed that the fungi could be grouped according to their physiological response...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1998.00368.x

    authors: Haasum I,Nielsen PV

    更新日期:1998-03-01 00:00:00

  • A 210-min solid phase cytometry test for the enumeration of Escherichia coli in drinking water.

    abstract::A 210-min-test for the enumeration of Escherichia coli in drinking water is described, based on solid phase cytometry (SPC) and a two-step enzymatic procedure for fluorescence labelling of single cells and small microcolonies. The test involves membrane filtration through a 25-mm black polyester filter, induction of b...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01125.x

    authors: Van Poucke SO,Nelis HJ

    更新日期:2000-09-01 00:00:00

  • Identification of pathogenic yeast species by polymerase chain reaction amplification of the RPS0 gene intron fragment.

    abstract:AIMS:This work focuses on the development of a method for the identification of pathogenic yeast. With this aim, we target the nucleotide sequence of the RPS0 gene of pathogenic yeast species with specific PCR primers. PCR analysis was performed with both the genomic DNA, whole cells of clinical isolates of Candida spe...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04595.x

    authors: Martínez JM,Gómez EV,Pemán J,Cantón E,García MG,del Castillo Agudo L

    更新日期:2010-06-01 00:00:00

  • PCR detection and 16S rRNA sequence-based phylogeny of a novel Propionibacterium acidipropionici applicable for enhanced fermentation of high moisture corn.

    abstract:AIMS:The aims of this study were to develop a sensitive and more rapid detection of Propionibacterium acidipropionici DH42 in silage and rumen fluid samples, and to explore its 16S rRNA sequence-based phylogeny. METHODS AND RESULTS:Nested polymerase chain reaction (PCR) was used with DH42-specific primers dhb1 and dhb...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02282.x

    authors: Romanov MN,Bato RV,Yokoyama MT,Rust SR

    更新日期:2004-01-01 00:00:00

  • Comparison of quantitative PCR and MALDI-TOF mass spectrometry assays for identification of bacteria in milk samples from cows with subclinical mastitis.

    abstract:AIMS:The objective of this study was to compare qualitatively and quantitatively the results of identification of the bacteria present in milk samples from cows with subclinical mastitis using multiplex qPCR assay and matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS® ) after ba...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14358

    authors: Ngassam Tchamba C,Rao AS,Boyen F,Haesebrouck F,Duprez JN,Théron L,Thiry D,Mainil JG

    更新日期:2019-09-01 00:00:00

  • Interleukin-8 gene expression and apoptosis induced by Salmonella Typhimurium in the presence of Bacillus probiotics in the epithelial cell.

    abstract:AIMS:This study aimed to evaluate the effects of three Bacillus probiotics on Salmonella Typhimurium, and interleukin-8 (IL-8) gene expression in the co-culture of the Bacillus and the pathogen in vitro. METHODS AND RESULTS:Bacillus subtilis, Bacillus indicus and Bacillus coagulans were initially turned to spore and h...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14898

    authors: Kawarizadeh A,Pourmontaseri M,Farzaneh M,Hosseinzadeh S,Ghaemi M,Tabatabaei M,Pourmontaseri Z,Pirnia MM

    更新日期:2020-10-15 00:00:00

  • Diversity among Bradyrhizobium isolates nodulating yardlong bean and sunnhemp in Guam.

    abstract:AIMS:To isolate and characterize bradyrhizobia that nodulate yardlong bean and sunnhemp in Guam. METHODS AND RESULTS:Bradyrhizobia populations that nodulate yardlong bean and sunnhemp in Guam were examined for genetic diversity and their relatedness to Bradyrhizobium japonicum and B. elkanii reference strains. Genomic...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01733.x

    authors: You Z,Marutani M,Borthakur D

    更新日期:2002-01-01 00:00:00

  • Sensory, chemical and bacteriological changes during storage of iced squid (Todaropsis eblanae).

    abstract:AIMS:To relate sensory shelf-life of iced whole and gutted squid to bacterial growth and chemical changes. METHODS AND RESULTS:Cooked mantles from whole and gutted individuals were rejected after 10 and 12 days of storage, respectively, due to ammoniacal off-odours. Rate of production of both ammonia and trimethylamin...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01604.x

    authors: Paarup T,Sanchez JA,Moral A,Christensen H,Bisgaard M,Gram L

    更新日期:2002-01-01 00:00:00

  • Bacillus anthracis surface: capsule and S-layer.

    abstract::Two abundant surface proteins, EA1 and Sap, are components of the Bacillus anthracis surface layer (S-layer). Their corresponding genes have been cloned, shown to be clustered on the chromosome and sequenced. EA1 and Sap each possess three 'S-layer homology' motifs. Single and double disrupted mutants were constructed...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00882.x

    authors: Fouet A,Mesnage S,Tosi-Couture E,Gounon P,Mock M

    更新日期:1999-08-01 00:00:00

  • The microstructure and distribution of micro-organisms within mature Serra cheese.

    abstract::The distribution of micro-organisms in mature Serra, a traditional Portuguese cheese made from unpasteurised ewes' milk without added starter culture, was examined by light microscopy and electron microscopy. Four populations of micro-organisms were recognized according to their position within the cheese: (i) those p...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1998.00375.x

    authors: Parker ML,Gunning PA,Macedo AC,Malcata FX,Brocklehurst TF

    更新日期:1998-04-01 00:00:00

  • Upgrading the phenolic content, antioxidant and antimicrobial activities of garden cress seeds using solid-state fermentation by Trichoderma reesei.

    abstract:AIMS:This study aims to evaluate the impact of solid-state fermentation (SSF) by Trichoderma reesei on the phenolic content, antioxidant and antimicrobial activities of garden cress seeds (GCS). METHODS AND RESULTS:The factorial statistical design was employed to optimize the SSF conditions, incubation time, pH, tempe...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14394

    authors: Abdel-Aty AM,Bassuiny RI,Barakat AZ,Mohamed SA

    更新日期:2019-11-01 00:00:00

  • Biofortification of riboflavin and folate in idli batter, based on fermented cereal and pulse, by Lactococcus lactis N8 and Saccharomyces boulardii SAA655.

    abstract:AIMS:Lactococcus lactis N8 and Saccharomyces boulardii SAA655 were investigated for their ability to synthesize B-vitamins (riboflavin and folate) and their functional role as microbial starters in idli fermentation. METHODS AND RESULTS:In this study, ultra-high performance liquid chromatography and microbiological as...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13453

    authors: Chandrasekar Rajendran SC,Chamlagain B,Kariluoto S,Piironen V,Saris PEJ

    更新日期:2017-06-01 00:00:00

  • Production and characterization of novel self-assembling biosurfactants from Aspergillus flavus.

    abstract:AIM:This work was conducted to produce, purify and characterize biosurfactants from Aspergillus flavus AF612 isolated from citrus fruit. METHODS AND RESULTS:Biosurfactant named 'Uzmaq' was isolated from A. flavus AF612. The chemical characterization of the biosurfactant was conducted. Biosurfactant Uzmaq produced by A...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12929

    authors: Ishaq U,Akram MS,Iqbal Z,Rafiq M,Akrem A,Nadeem M,Shafi F,Shafiq Z,Mahmood S,Baig MA

    更新日期:2015-10-01 00:00:00

  • Role of autoinducer-2 on the adhesion ability of Lactobacillus acidophilus.

    abstract:AIMS:Lactobacilli adhere to the intestinal epithelium and this intimate association likely promotes retention in the gastrointestinal tract and communication with the immune system. The aim of this study was to investigate whether or not the quorum-sensing signalling molecule, autoinducer (AI)-2, was produced by Lactob...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04204.x

    authors: Buck BL,Azcarate-Peril MA,Klaenhammer TR

    更新日期:2009-07-01 00:00:00

  • Presence of virulence genes, adhesion and invasion of Arcobacter butzleri.

    abstract:AIMS:The pathogenic potential of Arcobacter butzleri isolates was investigated by detecting the presence of putative virulence genes and analysing the adhesive and invasive capabilities in cell cultures of human cell lines. METHODS AND RESULTS:The presence of ten putative virulence genes in 52 A. butzleri isolates was...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12245

    authors: Karadas G,Sharbati S,Hänel I,Messelhäußer U,Glocker E,Alter T,Gölz G

    更新日期:2013-08-01 00:00:00

  • Evaluation of phenotypic and molecular typing techniques for determining diversity in Erwinia carotovora subspp. atroseptica.

    abstract::A number of phenotypic and molecular fingerprinting techniques, including physiological profiling (Biolog), restriction fragment length polymorphism (RFLP), enterobacterial repetitive intergenic consensus (ERIC) and a phage typing system, were evaluated for their ability to differentiate between 60 strains of Erwinia ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00929.x

    authors: Toth IK,Bertheau Y,Hyman LJ,Laplaze L,López MM,McNicol J,Niepold F,Persson P,Salmond GP,Sletten A,van Der Wolf JM,Pérombelon MC

    更新日期:1999-11-01 00:00:00

  • Which lactic acid bacteria are responsible for histamine production in wine?

    abstract:AIMS:To quantify the ability of 136 lactic acid bacteria (LAB), isolated from wine, to produce histamine and to identify the bacteria responsible for histamine production in wine. METHODS AND RESULTS:A qualitative method based on pH changes in a plate assay was used to detect wine strains capable of producing high lev...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02633.x

    authors: Landete JM,Ferrer S,Pardo I

    更新日期:2005-01-01 00:00:00

  • Influence of isomalto-oligosaccharides on intestinal microbiota in rats.

    abstract:AIMS:Isomalto-oligosaccharides (IMO) with α(1 --> 6) and α(1 --> 4) glucosidic linkages are produced by enzymatic conversion of starch. IMO are only partially digestible but data on their influence on intestinal microbiota are limited. It was the aim of this study to investigate the effect of IMO diet on intestinal mic...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.04984.x

    authors: Ketabi A,Dieleman LA,Gänzle MG

    更新日期:2011-05-01 00:00:00

  • Mechanisms of killing of Bacillus subtilis spores by Decon and Oxone, two general decontaminants for biological agents.

    abstract:AIMS:To determine the mechanisms of Bacillus subtilis spore killing by and resistance to the general biological decontamination agents, Decon and Oxone. METHODS AND RESULTS:Spores of B. subtilis treated with Decon or Oxone did not accumulate DNA damage and were not mutagenized. Spore killing by these agents was increa...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2004.02159.x

    authors: Young SB,Setlow P

    更新日期:2004-01-01 00:00:00

  • Production of laccases by Pleurotus ostreatus in submerged fermentation in co-culture with Trichoderma viride.

    abstract:AIMS:To evaluate the production and stability of laccases by Pleurotus ostreatus in liquid co-cultures with Trichoderma viride as a function of infection time and agitation rate. METHODS AND RESULTS:Pleurotus ostreatus cultures were infected with T. viride spores at 30 and 48 h. Maximal laccase volumetric activity was...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04493.x

    authors: Flores C,Casasanero R,Trejo-Hernández MR,Galindo E,Serrano-Carreón L

    更新日期:2010-03-01 00:00:00

  • Antifungal activity of tyrosol and farnesol used in combination against Candida species in the planktonic state or forming biofilms.

    abstract:AIM:This study examined the antifungal activity of the combination of tyrosol and farnesol against Candida albicans and Candida glabrata in the planktonic state or forming biofilms. METHODS AND RESULTS:The effect of drug association against Candida planktonic cells was assessed by the fractional inhibitory concentrati...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13513

    authors: Monteiro DR,Arias LS,Fernandes RA,Deszo da Silva LF,de Castilho MOVF,da Rosa TO,Vieira APM,Straioto FG,Barbosa DB,Delbem ACB

    更新日期:2017-08-01 00:00:00

  • Thyme essential oil as an alternative mechanism: biofungicide-causing sensitivity of Mycosphaerella graminicola.

    abstract:AIMS:To understand the mode of action of thyme essential oil as an alternative biofungicide. METHODS AND RESULTS:The chemical composition of thyme essential oil isolated by hydrodistillation from the aerial parts of Thymus vulgaris was analyzed. The main constituents of thyme essential oil were thymol (76·96%), ρ-cyme...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13408

    authors: Ben Jabeur M,Somai-Jemmali L,Hamada W

    更新日期:2017-04-01 00:00:00

  • Characterization of specific egg yolk immunoglobulin (IgY) against mastitis-causing Staphylococcus aureus.

    abstract:AIMS:To evaluate the in vitro activity of egg yolk immunoglobulin (IgY) against mastitis-causing Staphylococcus aureus. METHODS AND RESULTS:Specific IgY was produced by immunizing hens with formaldehyde-killed Staph. aureus, using a bacterial strain known to cause mastitis. The IgY, of 94% purity, was obtained from yo...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.03920.x

    authors: Zhen YH,Jin LJ,Guo J,Li XY,Li Z,Fang R,Xu YP

    更新日期:2008-11-01 00:00:00