Abstract：
:An environmental soil survey to detect Burkholderia pseudomallei was performed during the dry and wet seasons in Darwin, Northern Territory, Australia. Soil was sampled at regular intervals during a 15-month period at different depths from areas which were representative of the local, soil environment. Selective culture techniques using Ashdown's and Galimand and Dodin's methods and the polymerase chain reaction (PCR) using specific 16S rRNA primers were used to detect and identify the organism and determine its distribution within the soil stratum over the change in seasons. Results showed that Ashdown's method gave higher isolation rates in the dry season, and Galimand and Dodin's method gave higher isolation rates during the wet season. PCR of the soil enrichment proved to be a more sensitive method than culture and was also a useful confirmatory test in determining the identification of isolates where biochemical tests gave inconsistent results. The PCR primers were specific and able to detect 10(1) cfu g-1 soil and 10(4) cfu g-1 of soil using Ashdown's enrichment broth and Galimand and Dodin's broth, respectively. Overall the isolation of B. pseudomallei was greatest during the dry season and at the higher and lower soil depths, which is contradictory to epidemiological evidence that melioidosis occurs primarily during the wet season among patients exposed to contaminated surface soil and water.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Brook MD,Currie B,Desmarchelier PMsubject
Has Abstractpub_date
1997-05-01 00:00:00pages
589-96issue
5eissn
1364-5072issn
1365-2672journal_volume
82pub_type
杂志文章abstract：AIM:To isolate and identify an anticandida compound from newly isolated Fusarium strain MS-R1 and characterization of its activity against standard and clinical strains of Candida. METHODS AND RESULTS:The fungal strain, Fusarium strain MS-R1, was isolated from soil. Molecular identification according to the internal t...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12493
更新日期：2014-06-01 00:00:00
abstract：AIMS:In China, apple production areas are largely from the coastal to inland areas and across varied climate zones. However, the relationship among soil micro-organisms, environmental factors and fruit quality has not been clearly confirmed in orchards. Here we attempted to identify the variation of soil bacteria in th...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14878
更新日期：2020-10-04 00:00:00
abstract：AIMS:Although a link between agricultural cephalosporin use and resistance in Salmonella has been demonstrated with the drug ceftiofur, the underlying mechanism of the correlation is unclear. This study investigated the impact of ceftiofur exposure in S. Saintpaul on ceftriaxone resistance, the gene expression and the ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14749
更新日期：2020-12-01 00:00:00
abstract：AIMS:To generate continuous minimum inhibitory concentration (MIC) data that describes the discrete nature of experimentally derived population MIC data. METHODS AND RESULTS:A logistic model was fitted to experimentally derived MIC population cumulative distributions from clinical isolates of Haemophilus influenzae, M...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.02842.x
更新日期：2006-05-01 00:00:00
abstract：AIMS:To characterize airborne virus-like particles isolated from two cheese production plants in order to reveal their complexity in terms of viral communities and microbial genes potentially mobilized by viruses. METHODS AND RESULTS:Airborne virus-like particles have been isolated from Grana Padano and Gorgonzola PDO...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14046
更新日期：2018-11-01 00:00:00
abstract：:The primary mode of transmission of the human pathogen Helicobacter pylori is unresolved. This study examined the possibility that H. pylori is water-borne. Because methods for the direct culture of H. pylori from water samples remain elusive, a microscopic technique was used for detection of this organism. Actively r...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1999.00912.x
更新日期：1999-11-01 00:00:00
abstract：AIMS:To investigate whether Photobacterium damselae subsp. piscicida (Phdp) can sense and directly respond to the presence of cationic antimicrobial peptides (AMPs). METHODS AND RESULTS:We performed proteomic methodologies to investigate the responsive proteins of Phdp on exposure to AMP Q6. Proteins significantly alt...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12672
更新日期：2015-01-01 00:00:00
abstract：AIMS:Bacillus anthracis strains of various origins were analysed with the view to describe intrinsic and persistent structural components of the Bacillus collagen-like protein of anthracis glycoprotein associated anthrose containing tetrasaccharide in the exosporium. METHODS AND RESULTS:The tetrasaccharide consists of...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.04129.x
更新日期：2009-05-01 00:00:00
abstract：AIMS:To analyse the germination and its heterogeneity of individual spores of Clostridium perfringens. METHODS AND RESULTS:Germination of individual wild-type Cl. perfringens spores was followed by monitoring Ca-dipicolinic acid (CaDPA) release and by differential interference contrast (DIC) microscopy. Following the ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2011.05135.x
更新日期：2011-11-01 00:00:00
abstract：AIMS:To employ an in vitro screening regime to select a probiotic Bifidobacterium strain to complement resistant starch (Hi-maizetrade mark) in a synbiotic yoghurt. METHODS AND RESULTS:Of 40 Bifidobacterium isolates examined, only B. lactis Laftitrade mark B94 possessed all of the required characteristics. This isolat...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2001.01240.x
更新日期：2001-02-01 00:00:00
abstract：AIM:The aim of the present study was to investigate the in vitro antioxidant activity of yeast strains isolated from virgin olive oil. METHODS AND RESULTS:The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free-radical scavenging activity of single cultures of 24 yeast strains belonging to eight species isolated from virgin ol...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14520
更新日期：2020-03-01 00:00:00
abstract：AIMS:To evaluate the protective efficacy of Pediococcus pentosaceus GS4 in cadmium (Cd)-induced toxicity in Swiss albino mice. METHODS AND RESULTS:The previously reported probiotic P. pentosaceus GS4 interaction with Cd was evaluated experimentally. Swiss albino mice were segregated randomly into three different group...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14195
更新日期：2019-04-01 00:00:00
abstract：AIM:In the study, we investigated the regulatory effects of these genes (codY, comA, degU and spo0A) on the biosynthesis of three lipopeptides (bacillomycin D, fengycin and surfactin) in Bacillus amyloliquefaciens. METHODS AND RESULTS:The codY, comA, degU and spo0A genes in B. amyloliquefaciens fmbJ were knocked out. ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.15007
更新日期：2021-01-18 00:00:00
abstract：AIM:To apply a quantitative reverse-transcription PCR (qRT-PCR) method to determine the total viable count (TVC) on meat samples. METHODS AND RESULTS:Using two sets of primers to target the ribonuclease-P (RNase P) RNA transcripts of Gram-positive and Gram-negative bacteria, standard curves were generated using the Li...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04636.x
更新日期：2010-07-01 00:00:00
abstract：AIMS:To avoid interference by water-iodine disinfection chemistry and measure directly the effect of iodine, captured from a triiodide complex bound to a filter medium, on viability of penetrating viral particles. METHODS AND RESULTS:Aerosols of MS2 coli phage were passed through control P100 or iodinated High-Efficie...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12788
更新日期：2015-06-01 00:00:00
abstract：:Sixteen bacterial strains isolated from an activated sludge and Mycobacterium ssp. PYR-1 were tested for their ability to degrade polyaromatic hydrocarbons (PAHs). The bacterial strains Pasteurella ssp. (B-2) and Mycobacterium ssp. PYR-1 (AM) showed a high biodegradation potential of three- and four-ring PAHs. Bacteri...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1997.00261.x
更新日期：1997-11-01 00:00:00
abstract：AIMS:To estimate the efficacy of specific egg yolk immunoglobulin (IgY) for the treatment of lipopolysaccharide (LPS)-induced endotoxemia using a mouse model. METHODS AND RESULTS:Specific IgY was obtained from the yolk of hens immunized with formaldehyde-killed Escherichia coli O111 and showed a high binding activity ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2011.05155.x
更新日期：2011-12-01 00:00:00
abstract：AIMS:Environmental contamination plays an important role in the transmission of infections, especially healthcare-associated infections. Disinfection transiently reduces contamination, but surfaces can rapidly become re-contaminated. Antimicrobial surfaces may partially overcome that limitation. The antimicrobial activ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12308
更新日期：2013-11-01 00:00:00
abstract：AIMS:To study the phenotypic characteristics of Aeromonas spp. from environmental and clinical samples in Spain and to cluster these strains by numerical taxonomy. METHODS AND RESULTS:A collection of 202 Aeromonas strains isolated from bivalve molluscs, water and clinical samples was tested for 64 phenotypic propertie...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2002.01711.x
更新日期：2002-01-01 00:00:00
abstract：AIMS:Most studies of biofilm effects on dental materials use single-species biofilms, or consortia. Microcosm biofilms grown directly from saliva or plaque are much more diverse, but difficult to characterize. We used the Human Oral Microbial Identification Microarray (HOMIM) to validate a reproducible oral microcosm m...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2012.05439.x
更新日期：2012-12-01 00:00:00
abstract：AIMS:To develop methods to differentiate Escherichia coli O157:H7 and related serotypes by the use of amplicon length polymorphism (ALP) analysis based on identifying DNA sequence deletions within highly homologous regions of three sequenced E. coli strains. METHODS AND RESULTS:Potential primer locations along the anc...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.03987.x
更新日期：2009-01-01 00:00:00
abstract：AIM:To determine if disinfecting footbaths in the food industry were contaminated with bacteria and to characterize some of the bacteria present. METHODS AND RESULTS:Bacterial strains were isolated from disinfecting footbaths containing TEGO 103G (amphoteric disinfectant) or TP-99 (alkyl amino acetate-based disinfecta...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2003.01968.x
更新日期：2003-01-01 00:00:00
abstract：AIMS:To determine the mechanisms of Bacillus subtilis spore resistance to and killing by a novel sporicide, dimethyldioxirane (DMDO) that was generated in situ from acetone and potassium peroxymonosulfate at neutral pH. METHODS AND RESULTS:Spores of B. subtilis were effectively killed by DMDO. Rates of killing by DMDO...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.03000.x
更新日期：2006-11-01 00:00:00
abstract：:The exploration of new source materials and the use of alternative isolation and identification methods have led to rapid expansion in the knowledge of diversity; in Lysobacter, 11 new species having been described since 2005, and in Stenotrophomonas with six new species since 2000. The new species of Lysobacter, isol...
journal_title：Journal of applied microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2672.2009.04471.x
更新日期：2010-03-01 00:00:00
abstract：AIMS:Recombinant Saccharomyces cerevisiae strains harbouring different levels of xylulokinase (XK) activity and effects of XK activity on utilization of xylulose were studied in batch and fed-batch cultures. METHODS AND RESULTS:The cloned xylulokinase gene (XKS1) from S. cerevisiae was expressed under the control of t...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2003.02055.x
更新日期：2003-01-01 00:00:00
abstract：AIMS: The aim of this study was to generate temperature-sensitive Edwardsiella tarda mutant and to evaluate potential of the mutant as a vaccine in olive flounder (Paralichthys olivaceus). METHODS AND RESULTS: A temperature-sensitive E. tarda mutant was generated by replacement of alr gene promoter with cI857-λP(R) pr...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2012.05328.x
更新日期：2012-08-01 00:00:00
abstract：AIMS:Silage is grass, preserved by fermentation and used as winter feed for cattle. The impact of a range of current grass silage preparation practices on the survival of Escherichia coli C600φ3538(Δvtx2 ::cat) and on the induction, release and infectivity of free phage were investigated. METHODS AND RESULTS:Wilted an...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13431
更新日期：2017-05-01 00:00:00
abstract：AIMS:To determine the effects of supplemented copper (Cu2+) on growth and viability of strains used as starters and adjunct cultures for Emmental cheese manufacture. METHODS AND RESULTS:Thirteen strains belonging to Lactobacillus delbrueckii, Lactobacillus helveticus, Lactobacillus rhamnosus, Streptococcus thermophilu...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.03849.x
更新日期：2008-10-01 00:00:00
abstract：AIMS:To determine the effect of antimicrobial selective pressure on the transmission of antimicrobial resistant and sensitive strains of Salmonella in poultry. METHODS AND RESULTS:Eight pens housed 12 broiler chicks each. Two chicks in four of the pens were inoculated with a Salm. Typhimurium strain resistant to 12 an...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.03036.x
更新日期：2006-12-01 00:00:00
abstract：AIMS:The study aims to demonstrate the antimicrobial and disinfectant resistance phenotypes and genotypes of Escherichia coli isolates obtained from giant pandas (Ailuropoda melanoleuca). METHODS AND RESULTS:Antimicrobial testing was performed according to the standard disk diffusion method. The minimal inhibitory con...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12820
更新日期：2015-07-01 00:00:00
