A novel echinocandin MIG0310 with anticandida activity from newly isolated Fusarium sp. strain MS-R1.

Abstract:

AIM:To isolate and identify an anticandida compound from newly isolated Fusarium strain MS-R1 and characterization of its activity against standard and clinical strains of Candida. METHODS AND RESULTS:The fungal strain, Fusarium strain MS-R1, was isolated from soil. Molecular identification according to the internal transcribed spacer region of the rRNA gene sequence showed the strain to be strongly related to Fusarium brachygibbosum complex. Successive extractions of the active compound showed activity against Candida albicans, including clinical strains. Inhibition of a clinical strain of Candida tropicalis, but not Candida krusei and Candida glabrata, was also shown as tested by the broth microdilution assay. The compound was purified by liquid chromatography coupled with thin-layer chromatography and bioautography and characterized by nuclear magnetic resonance spectroscopy. It was confirmed to have the molecular formula C(48)H(66)O(18) and was identified as an echinocandin with a novel structure. CONCLUSION:A novel echinocandin-type antifungal metabolite, MIG0310, was isolated and characterized. This is a second echinocandin reported from a Fusarium species, indicating this genus to have wider range of echinocandin compounds. SIGNIFICANCE AND IMPACT OF THE STUDY:The new compound may lead to new anticandidal drugs, broadening the treatment opportunities for Candida species, including those that are resistant to the current antifungal drugs.

journal_name

J Appl Microbiol

authors

Masaphy S

doi

10.1111/jam.12493

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

1458-64

issue

6

eissn

1364-5072

issn

1365-2672

journal_volume

116

pub_type

杂志文章
  • Phenotypic and genotypic profile of clinical and animal multidrug-resistant Salmonella enterica isolates from Mexico.

    abstract:AIMS:The objective of this study was to obtain a phenotypic and genotypic profile of Salmonella enterica including multidrug-resistant (MDR) isolates from food-producing animals and clinical isolates, as well as their genetic relatedness in two different States of Mexico (Jalisco and State of Mexico). METHODS AND RESU...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13615

    authors: Aguilar-Montes de Oca S,Talavera-Rojas M,Soriano-Vargas E,Barba-León J,Vázquez-Navarrete J,Acosta-Dibarrat J,Salgado-Miranda C

    更新日期:2018-01-01 00:00:00

  • Monitoring of Staphylococcus xylosus DSM 20266 added as starter during fermentation and ripening of soppressata molisana, a typical Italian sausage.

    abstract:AIMS:"Soppressata molisana", a fermented sausage produced in southern Italy, is commonly obtained without starter addition. However, the use of starter cultures is more and more recommended in meat fermentation processes in order to guarantee stable production performance. In this study, the survival of the Staphylococ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01512.x

    authors: Di Maria S,Basso AL,Santoro E,Grazia L,Coppola R

    更新日期:2002-01-01 00:00:00

  • Inactivation of Bacillus subtilis spores by combining ultrasonic waves under pressure and mild heat treatment.

    abstract::The inactivation of Bacillus subtilis spores by ultrasonic treatments under static pressure (Mano-Sonication, MS) and a combined MS/heat treatment (Mano-Thermo-Sonication) was investigated. The sporicidal effect of MS treatments depended on static pressure, amplitude of ultrasonic waves and treatment temperature. At 7...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1998.00593.x

    authors: Raso J,Palop A,Pagán R,Condón S

    更新日期:1998-11-01 00:00:00

  • A method to evaluate factors influencing the microbial reduction in domestic dishwashers.

    abstract:AIMS:To develop a method that is able to determine the microbial reduction in different dishwasher cleaning cycles and differentiate between different program parameters used. METHODS AND RESULTS:Stainless steel biomonitors were contaminated with Micrococcus luteus or Entereococcus faecium and cleaned in a specially p...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14564

    authors: Brands B,Schulze Struchtrup S,Stamminger R,Bockmühl DP

    更新日期:2020-05-01 00:00:00

  • Effects of organic loading rate on hydrogen and volatile fatty acid production and microbial community during acidogenic hydrogenesis in a continuous stirred tank reactor using molasses wastewater.

    abstract:AIMS:Microbial community associated with hydrogen production and volatile fatty acids (VFAs) accumulation was characterized in acidogenic hydrogenesis using molasses wastewater as a feedstock. METHODS AND RESULTS:Hydrogen and VFAs production were measured under an organic loading rate (OLR) from 19 to 35 g-COD l-1  da...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13316

    authors: Yun J,Cho KS

    更新日期:2016-12-01 00:00:00

  • Diversity, dynamics and activity of bacterial populations in 'Registered Designation of Origin' Salers cheese by single-strand conformation polymorphism analysis of 16S rRNA genes.

    abstract:AIMS:The aim of this work was to measure the dynamic global metabolic activities of the microbial community during ripening of RDO Salers cheese by using a direct molecular approach. METHODS AND RESULTS:A culture-independent approach including PCR, reverse transcriptase PCR (RT-PCR) and single strand conformation poly...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02575.x

    authors: Duthoit F,Tessier L,Montel MC

    更新日期:2005-01-01 00:00:00

  • Anthrax lethal factor cleaves the N-terminus of MAPKKS and induces tyrosine/threonine phosphorylation of MAPKS in cultured macrophages

    abstract::The lethal toxin (LeTx) of Bacillus anthracis is the major virulence factor responsible for the death of infected animals and for cytolysis of cultured macrophages. Its catalytic component, LF, contains the characteristic zinc-binding motif of metalloproteases, it binds zinc and indirect evidence suggests that this hy...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00893.x

    authors: Vitale G,Pellizzari R,Recchi C,Napolitani G,Mock M,Montecucco C

    更新日期:1999-08-01 00:00:00

  • Genetic determinates for conjugated linolenic acid production in Lactobacillus plantarum ZS2058.

    abstract:AIMS:To investigate the genetic determinates for conjugated linolenic acid (CLNA) production in Lactobacillus plantarum ZS2058, a high CLNA producer. METHODS AND RESULTS:After culturing with α-linolenic acid (ALA) in the medium, the fatty acid compositions of supernatant fluid and cell pellets were analysed via GC-MS....

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14466

    authors: Yang B,Chen H,Gao H,Ren Q,Zhang H,Chen W

    更新日期:2020-01-01 00:00:00

  • Regulation of alkaline metalloprotease promoter by N-acyl homoserine lactone quorum sensing in Pseudomonas fluorescens.

    abstract:AIMS:To examine the involvement of N-acyl homoserine lactone (AHL) quorum sensing in alkaline metalloprotease (aprX) promoter regulation in a Pseudomonas fluorescens milk isolate. METHODS AND RESULTS:N-acyl homoserine lactone signals from P. fluorescens strain 395 were separated and detected by thin layer chromatograp...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03488.x

    authors: Liu M,Wang H,Griffiths MW

    更新日期:2007-12-01 00:00:00

  • A novel in vitro assay for assessing efficacy and toxicity of antifungals using human leukaemic cells infected with Candida albicans.

    abstract:AIMS:This study describes a novel in vitro assay that simultaneously determines antifungal efficiency and host cell toxicity using suspensions of human leukaemic cells (HL-60) infected with Candida albicans. METHODS AND RESULTS:The effect of Candida infection on host cell viability was evaluated by the microscopy of t...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12817

    authors: Boros-Majewska J,Turczyk Ł,Wei X,Milewski S,Williams DW

    更新日期:2015-07-01 00:00:00

  • Microbiological composition of raw milk from selected farms in the Camembert region of Normandy.

    abstract::Raw milk from 27 farms was sampled over 6 months for listerias, salmonellas, Yersinia enterocolitica and campylobacters. Total bacterial counts and somatic cell counts were measured. Lactococci, lactobacilli, dextran-producing leuconostocs, Brevibacterium linens, yeasts and moulds, Staphylococcus aureus and other Micr...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1997.00166.x

    authors: Desmasures N,Bazin F,Guéguen M

    更新日期:1997-07-01 00:00:00

  • Synergistic mode of action of catechin, vanillic and protocatechuic acids to inhibit the adhesion of uropathogenic Escherichia coli on silicone surfaces.

    abstract:AIMS:To study the individual and combined contribution of catechin, protocatechuic and vanillic acids to inhibit the adhesion of uropathogenic Escherichia coli (UPEC) on the surface of silicone catheters. METHODS AND RESULTS:The adhesion of UPEC to silicone catheters during the exposure to nonlethal concentrations of ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14472

    authors: Bernal-Mercado AT,Gutierrez-Pacheco MM,Encinas-Basurto D,Mata-Haro V,Lopez-Zavala AA,Islas-Osuna MA,Gonzalez-Aguilar GA,Ayala-Zavala JF

    更新日期:2020-02-01 00:00:00

  • Characterization of sourdough lactic acid bacteria based on genotypic and cell-wall protein analyses.

    abstract:AIMS:To evaluate the effectiveness of two independent methods in differentiating a large population of lactic acid bacteria (LAB) isolated from wheat flours and sourdoughs and to correlate eventual differences/similarities among strains with their geographical origin and/or process parameters. METHODS AND RESULTS:One ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01874.x

    authors: Corsetti A,De Angelis M,Dellaglio F,Paparella A,Fox PF,Settanni L,Gobbetti M

    更新日期:2003-01-01 00:00:00

  • Morphological and phenotypical characterization of Bacillus sporothermodurans.

    abstract:AIMS:Enumeration of resistant bacteria in ultra-high temperature (UHT) treated milk; morphological characterization and phenotyping of resistant strains by traditional and nontraditional methods and their identification by molecular biology. METHODS AND RESULTS:Modified standard plate count agar (PCA) and modified bra...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02371.x

    authors: Montanari G,Borsari A,Chiavari C,Ferri G,Zambonelli C,Grazia L

    更新日期:2004-01-01 00:00:00

  • Involvement of a rhamnolipid-producing strain of Pseudomonas aeruginosa in the degradation of polycyclic aromatic hydrocarbons by a bacterial community.

    abstract::A rhamnolipid-producing strain of Pseudomonas aeruginosa GL1 was isolated from a bacterial community growing on a mixture of polycyclic aromatic hydrocarbons (PAH) as sole carbon source. Strain GL1 did not grow on PAH but grew on known degradation metabolites of phenanthrene (O-phthalic acid) and of naphthalene (salic...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1998.00412.x

    authors: Arino S,Marchal R,Vandecasteele JP

    更新日期:1998-05-01 00:00:00

  • Application of deep neural techniques in predictive modelling for the estimation of Escherichia coli growth rate.

    abstract:AIMS:To develop a predictive model for Escherichia coli using deep neural networks. METHODS AND RESULTS:Batch experiments are conducted at different temperatures closer to optimum value (36·5°C, 37°C, 37·5°C, 38°C and 38·5°C) to obtain the growth curves of E .coli K-12. Two primary models namely modified Gompertz and ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14901

    authors: Chitra M,Sutha S,Pappa N

    更新日期:2020-10-16 00:00:00

  • Molecular diversity of rhizobia nodulating the invasive legume Cytisus scoparius in Australia.

    abstract:AIMS:To contribute to the understanding of Cytisus scoparius success at invading and establishing itself in Australia. METHODS AND RESULTS:Root-nodule bacteria isolated from C. scoparius, growing on five different sites and originally introduced to Australia, were compared with isolates from indigenous plants growing ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.02902.x

    authors: Lafay B,Burdon JJ

    更新日期:2006-06-01 00:00:00

  • Preparation of an inoculum of Gluconacetobacter xylinus without mutants in shaken culture.

    abstract:AIMS:A high-quality inoculum of Gluconacetobacter xylinus is important to produce bacterial cellulose (BC), a versatile biomaterial. This work aims to develop a method of preparing an inoculum of this bacterium with high cell density and without mutants. METHODS AND RESULTS:Inocula of G. xylinus ACCC 10220 without and...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13193

    authors: Wang ZG,Xiang D,Wang XB,Li CF

    更新日期:2016-09-01 00:00:00

  • An eight-month study of a population of verocytotoxigenic Escherichia coli (VTEC) in a Scottish cattle herd.

    abstract:AIMS:Strains of Verocytotoxin-producing Escherichia coli (VTEC) from Scottish beef cattle on the same farm were isolated during four visits over a period of eight months. Characteristics of these strains were examined to allow comparisons with strains of VTEC associated with human infection. METHODS AND RESULTS:Strain...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01771.x

    authors: Jenkins C,Pearce MC,Chart H,Cheasty T,Willshaw GA,Gunn GJ,Dougan G,Smith HR,Synge BA,Frankel G

    更新日期:2002-01-01 00:00:00

  • Archaeal diversity in naturally occurring and impacted environments from a tropical region.

    abstract:AIMS:To evaluate archaeal diversity in natural and impacted habitats from Rio de Janeiro state, Brazil, a tropical region of South America. METHODS AND RESULTS:16S rRNA gene was amplified directly by polymerase chain reaction (PCR) from genomic DNA, extracted from Guanabara Bay (GB) water, halomarine sediment (HS), mu...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03230.x

    authors: Clementino MM,Fernandes CC,Vieira RP,Cardoso AM,Polycarpo CR,Martins OB

    更新日期:2007-07-01 00:00:00

  • Quantification and genetic diversity of total and microcystin-producing Microcystis during blooming season in Tai and Yang-cheng lakes, China.

    abstract:AIMS:The aims of present study were to evaluate the abundances, genetic diversity of total and microcystin-producing Microcystis over temporal and spatial scales, and to investigate relationships among Microcystis and water parameters in Tai and Yang-cheng lakes. METHODS AND RESULTS:Abundances of total and microcystin...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12456

    authors: Li D,Gu AZ,He M

    更新日期:2014-06-01 00:00:00

  • Detection of the nifH gene of Methanobrevibacter smithii: a potential tool to identify sewage pollution in recreational waters.

    abstract:AIMS:The goal of this study was to develop and test the efficacy of a PCR assay for the environmental detection of the nifH gene of Methanobrevibacter smithii, a methanogen found in human faeces and sewage. METHODS AND RESULTS:PCR primers for the nifH gene of M. smithii were designed, tested and used to detect the pre...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.02989.x

    authors: Ufnar JA,Wang SY,Christiansen JM,Yampara-Iquise H,Carson CA,Ellender RD

    更新日期:2006-07-01 00:00:00

  • Physicochemical characterization of pectinase activity from Bacillus spp. and their accessory role in synergism with crude xylanase and commercial cellulase in enzyme cocktail mediated saccharification of agrowaste biomass.

    abstract:AIM:The aim of this study was to evaluate the physicochemical properties of the crude pectinase activity from three Bacillus isolates of ruminant dung origin and study their synergism with crude xylanases from the same Bacillus spp. and a commercial cellulase to evaluate their accessory role in improved biomass sacchar...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13718

    authors: Thite VS,Nerurkar AS

    更新日期:2018-05-01 00:00:00

  • Effectiveness of egg yolk immunoglobulin against the intracellular salmonid pathogen Piscirickettsia salmonis.

    abstract:AIMS:To produce and characterize egg yolk immunoglobulin (IgY) against the fish intracellular pathogen Piscirickettsia salmonis as well as to evaluate the antibacterial activity of IgY in vitro and the availability in the serum of fish immunized orally. METHODS AND RESULTS:Specific IgY was produced by immunizing hens ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12857

    authors: Oliver C,Valenzuela K,Silva H,Haro RE,Cortés M,Sandoval R,Pontigo JP,Álvarez C,Figueroa JE,Avendaño-Herrera R,Troncoso JM,Yáñez AJ

    更新日期:2015-08-01 00:00:00

  • Preliminary analysis of Chloroflexi populations in full-scale UASB methanogenic reactors.

    abstract:AIMS:The phylum Chloroflexi is frequently found in high abundance in methanogenic reactors, but their role is still unclear as most of them remain uncultured and understudied. Hence, a detailed analysis was performed in samples from five up-flow anaerobic sludge blanket (UASB) full-scale reactors fed different industri...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14115

    authors: Bovio P,Cabezas A,Etchebehere C

    更新日期:2019-02-01 00:00:00

  • Hi-tech restoration by two-steps biocleaning process of Triumph of Death fresco at the Camposanto Monumental Cemetery (Pisa, Italy).

    abstract:AIMS:In this work, the 'hi-tech' complex biocleaning and restoration of the 14th-century fresco Triumph of Death (5·6 × 15·0 m) at the Camposanto Monumental Cemetery (Pisa, Italy) is reported. Since 2000, the restoration based on the biological cleaning of noble medieval frescoes, has been successfully utilized in this...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13913

    authors: Ranalli G,Zanardini E,Andreotti A,Colombini MP,Corti C,Bosch-Roig P,De Nuntiis P,Lustrato G,Mandrioli P,Rampazzi L,Giantomassi C,Zari D

    更新日期:2018-09-01 00:00:00

  • Development of antimicrobial nanoemulsion-based delivery systems against selected pathogenic bacteria using a thymol-rich Thymus daenensis essential oil.

    abstract:AIMS:Thymol-rich medicinal plants have been used in traditional medicine to relieve infectious diseases. However, the application of essential oils as medicine is limited by its low water solubility and high vapour pressure. The objective of this study was to produce stable nanoemulsions of Thymus daenensis oil in wate...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13541

    authors: Ghaderi L,Moghimi R,Aliahmadi A,McClements DJ,Rafati H

    更新日期:2017-10-01 00:00:00

  • Comparison of selective and non-selective enrichment media in the detection of Listeria monocytogenes from meat containing Listeria innocua.

    abstract:AIMS:This study investigated whether the higher incidence of recovery from meat of Listeria innocua compared with L. monocytogenes could be due to the laboratory media used, leading to an artificially lower detection of the pathogenic species, L. monocytogenes. METHODS AND RESULTS:Minced beef was inoculated with L. mo...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01336.x

    authors: Duffy G,Walsh D,Sheridan JJ,Logue CM,Harrington D,Blair IS,McDowell DA

    更新日期:2001-06-01 00:00:00

  • Fungitoxicity of organic extracts of Ocimum basilicum on growth and morphogenesis of Bipolaris species (teleomorph Cochliobolus).

    abstract:AIMS:This study aimed at evaluating the inhibitory effects of various organic extracts of Ocimum basilicum against some species of Bipolaris and Cochliobolus with GC-MS and HPLC analysis. METHODS AND RESULTS:The ethyl acetate extract consisted of methyl cinnamate as the most abundant component, while butylated hydroxy...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13543

    authors: Elsherbiny EA,Safwat NA,Elaasser MM

    更新日期:2017-10-01 00:00:00

  • Metabolically active microbial communities of yellow and grey colonizations on the walls of Altamira Cave, Spain.

    abstract:AIMS:To determine the major components of total and metabolically active microbial communities of yellow and grey colonizations threatening the conservation of palaeolithic paintings in Altamira Cave (Spain). METHODS AND RESULTS:Micro-organisms present in yellow and grey colonizations were determined from DNA analysis...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03594.x

    authors: Portillo MC,Gonzalez JM,Saiz-Jimenez C

    更新日期:2008-03-01 00:00:00