Abstract:
AIMS:Thymol-rich medicinal plants have been used in traditional medicine to relieve infectious diseases. However, the application of essential oils as medicine is limited by its low water solubility and high vapour pressure. The objective of this study was to produce stable nanoemulsions of Thymus daenensis oil in water by preventing Ostwald ripening and phase separation. METHODS AND RESULTS:The antibacterial activity of bulk and emulsified essential oil against selected pathogenic bacteria including Gram-negative (Haemophilus influenzae, Pseudomonas aeruginosa) and Gram-positive (Streptococcus pneumoniae) were investigated in the liquid and vapour phase. The optimum formulation (L2) contained 2% Tween 80 (surfactant) and 0·1% lecithin (cosurfactant) had a mean droplet diameter of 131 nm. In the liquid phase, the optimized nanoemulsion exhibited good antibacterial activity against S. pneumonia with MIC value of 0·0039 mg mL-1 . In the vapour phase, the MIC values against S. pneumonia were similar (<7·35 μL L-1 ) for both bulk and emulsified essential oil. However, there was no antibacterial activity in the vapour phase against H. influenzae and P. aeruginosa. Analysis of thymol concentration in the head space indicated that the nanoemulsion retarded the release of thymol into the vapour phase. CONCLUSIONS:These findings highlight the potential applications of nanoemulsions containing essential oils as antibacterial products. SIGNIFICANCE AND IMPACT OF THE STUDY:The results of the current study highlight the advantages of nanoemulsification for improvement of the physicochemical properties and the antibacterial activity of T. daenensis EOs in the liquid and vapour phase for therapeutic purposes.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Ghaderi L,Moghimi R,Aliahmadi A,McClements DJ,Rafati Hdoi
10.1111/jam.13541subject
Has Abstractpub_date
2017-10-01 00:00:00pages
832-840issue
4eissn
1364-5072issn
1365-2672journal_volume
123pub_type
杂志文章abstract:AIMS:This paper describes optimization of electrotransformation of Mu transposition complexes into Lactococcus lactis cells and identification of genes affecting nisin production. METHODS AND RESULTS:The highest transformation efficiency, 1.1 x 10(2) transformants microg(-1) of input transposon DNA, was achieved when ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03962.x
更新日期:2009-01-01 00:00:00
abstract:AIMS:The present work aimed to obtain bioinsecticide over-producing mutants through classical mutagenesis of vegetative cells of Bacillus thuringiensis (Bt) by using u.v. and nitrous acid, and to evidence the involvement of cell metabolism in delta-endotoxin synthesis. METHODS AND RESULTS:Vegetative cells of Bt were t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02323.x
更新日期:2004-01-01 00:00:00
abstract::Pseudomonas fluorescens RO98, a raw milk isolate, was inoculated into McKellar's minimal salts medium and incubated at 25 degrees C for 48 h to allow production of protease. A zinc-metalloacid protease was purified from the cell-free concentrate by anion exchange and gel filtration chromatography. The purified proteas...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01108.x
更新日期:2000-08-01 00:00:00
abstract:AIMS:We have been for a species of thermophilic bacteria that can effectively decompose collagen and collagen peptides that tend to be hard-to-degrade proteins because of their high content of proline residues. This study focused upon the enzymatic degradation of prolyl peptides by thermophilic bacteria. METHODS AND R...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02210.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To investigate the sort and the origin of the contamination of a packed fruit juice. METHODS AND RESULTS:Fifty-eight yeast isolates were collected in a survey of two different visits to a carbonated orange juice factory. In each visit, samples were collected, six times, from seven points in the production chain. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02542.x
更新日期:2005-01-01 00:00:00
abstract:AIM:To investigate the influence of several environmental factors on the viability and cell-adsorption for two Lactobacillus casei/paracasei bacteriophages (PL-1 and J-1). METHODS AND RESULTS:Both phages showed a remarkably high specificity of species, sharing similar host spectra. Two phages and four sensitive strain...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02767.x
更新日期:2006-02-01 00:00:00
abstract:AIM:To describe the relationship between the concentration of different indicator bacteria in red meat. METHODS AND RESULTS:Enumeration data for aerobic plate count (APC), Enterobacteriaceae, coliforms and Escherichia coli biotype I were analysed from an Australia-wide survey of beef carcasses, sheep carcasses, frozen...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03060.x
更新日期:2007-01-01 00:00:00
abstract:AIMS:Develop an effective laboratory method to consistently recover viral loads from porous concrete coupons sufficient for disinfectant efficacy testing. Investigate the role of concrete matrix pH on the recovery of foot-and-mouth disease virus (FMDV) and African Swine Fever virus (ASFV) from porous concrete. Compare ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14694
更新日期:2020-11-01 00:00:00
abstract:AIMS:To obtain the high-yield strain of fusidic acid, which is produced from fungus Fusidium coccineum and is the only fusidane-type antibiotic that has been used clinically, and confirm the changes in the transcription levels involved in increasing its production. METHODS AND RESULTS:By using the atmospheric and room...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14797
更新日期:2020-07-30 00:00:00
abstract:AIMS:To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. METHODS AND RESULTS:The specificity of a developed 5' nuclease rt-PCR for species-specific identification of Campylobacter jejuni, Campyloba...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02616.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:The purpose of this study was to isolate, identify and characterize an antifungal compound from Lactobacillus plantarum KCC-10 from forage silage with potential beneficial properties. METHODS AND RESULTS:The 16S rRNA gene-based phylogenetic affiliation was determined using bioinformatic tools and identified as La...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12319
更新日期:2013-11-01 00:00:00
abstract:AIMS:To improve lincomycin A production and decrease the content of byproduct lincomycin B in an industrial lincomycin-producing strain. METHODS AND RESULTS:The in silico analysis indicated that LmbW could be involved in propylproline biosynthesis of lincomyin A. In this study, we constructed an lmbW deletion mutant a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12919
更新日期:2015-10-01 00:00:00
abstract:AIM:This study investigates the antimicrobial effects of fish oil-based formulas rich in omega-3 fatty acids (free fatty acids, ethyl esters or triacylglycerols), against cystic fibrosis (CF) pathogens (Burkholderia cenocepacia K56-2 and Pseudomonas aeruginosa PAO1), often resistant to multiple antibiotics. METHODS AN...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13145
更新日期:2016-06-01 00:00:00
abstract:AIM:The aim of this study was to develop and evaluate bacterial-fungal communities to deconstruct switchgrass to biofuel precursors. METHODS AND RESULTS:Bacterial-fungal consortia, mesophilic (25°C) and thermophilic (50°C), were enriched from switchgrass bales from which enzyme mixtures were used to deconstruct delign...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13295
更新日期:2017-04-01 00:00:00
abstract:AIMS:This study elucidates the mechanisms by which a nonbacteriocinogenic Carnobacterium piscicola inhibits growth of Listeria monocytogenes. METHODS AND RESULTS:Listeria monocytogenes was exposed to live cultures of a bacteriocin-negative variant of C. piscicola A9b in co-culture, in a diffusion chamber system, and t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02438.x
更新日期:2005-01-01 00:00:00
abstract::In order to characterize the subtypes of Salmonella typhi which cause sporadic disease in Taiwan, 55 isolates of Salm. typhi obtained from unrelated patients of sporadic cases during 1992-96 were subjected to chromosomal DNA digestion and pulsed field gel electrophoresis (PFGE). When DNAs of these 55 Salm. typhi strai...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00720.x
更新日期:1999-05-01 00:00:00
abstract::NaCl-tolerant lactic acid bacteria (LAB) strains LC-10 (Lactobacillus casei) and LP-15 (Lact. plantarum) and NaCl were used as additives to sorghun (Sorghum bicolor). Numbers of LAB were significantly (P < 0.05) higher in all the additive-treated silages than in the control silage at an early stage of ensiling. During...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00229.x
更新日期:1997-09-01 00:00:00
abstract:AIMS:Poor colonization of the rhizosphere is a major constraint of seed treatment biological control. The objectives of this study were to; examine the colonization of the rhizosphere of sugar beet seedlings by selected rhizobacteria; determine the influence of the host rhizosphere and percolating water on the distribu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01523.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To evaluate archaeal diversity in natural and impacted habitats from Rio de Janeiro state, Brazil, a tropical region of South America. METHODS AND RESULTS:16S rRNA gene was amplified directly by polymerase chain reaction (PCR) from genomic DNA, extracted from Guanabara Bay (GB) water, halomarine sediment (HS), mu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03230.x
更新日期:2007-07-01 00:00:00
abstract:AIMS:Bacillus licheniformis PWD-1 is a keratin-degrading, spore-forming bacterium isolated from a poultry waste digester. A sporulation-deficient mutant of B. licheniformis PWD-1, named B. licheniformis WBG, was developed and characterized. METHODS AND RESULTS:The mutation was generated using the splicing by overlap e...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02515.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:Investigating mechanisms of lethality enhancement when Escherichia coli O157:H7, and selected E. coli mutants, were exposed to tert-butylhydroquinone (TBHQ) during ultra-high pressure (UHP) treatment. METHODS AND RESULTS:Escherichia coli O157:H7 EDL-933, and 14 E. coli K12 strains with mutations in selected genes...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03973.x
更新日期:2008-12-01 00:00:00
abstract::Bacterial isolates from bean-sprouts were screened for anti-Listeria monocytogenes bacteriocins using a well diffusion method. Thirty-four of 72 isolates inhibited the growth of L. monocytogenes Scott A. One, HPB 1688, which had the biggest inhibition zone against L. monocytogenes Scott A, was selected for subsequent ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00262.x
更新日期:1997-10-01 00:00:00
abstract:AIMS:The ComPath project is a pan-European programme dedicated to the monitoring of antimicrobial susceptibility of pathogens from diseased dogs and cats using standardized methods and centralized minimum inhibitory concentration (MIC) determination. Here, the susceptibility of major pathogens is reported from antimicr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13287
更新日期:2016-11-01 00:00:00
abstract:AIMS:To screen and select the Bacillus spp. from Tua-nao of northern Thailand for fermented corticate soybean meal (FCSBM) production. METHODS AND RESULTS:After isolation of Bacillus spp. from Tua-nao was carried out, cellulase, hemicellulases (i.e., β-mannanase and xylanase) and phytase production by isolated Bacillu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05395.x
更新日期:2012-10-01 00:00:00
abstract:AIMS:To assess the presence of virulence genes in environmental and foodborne Escherichia coli isolates using the TaqMan PCR system. METHODS AND RESULTS:Three TaqMan pathogen detection kits called O157:H7, StxI and StxII were used to investigate the presence of virulence genes in Escherichia coli isolates. All 54 food...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02023.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:The objective of the present work was to describe an aerobic, mesophilic and heterotrophic marine bacterium, designated HYD657, able to produce an exopolysaccharide (EPS). It was isolated from a East Pacific Rise deep-sea hydrothermal vent polychaete annelid. METHODS AND RESULTS:This micro-organism, on the basis ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01689.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To assess the behaviour of Aspergillus section Nigri IOC 4573 in mango nectar as affected by temperature and pH. METHODS AND RESULTS:A central composite design (2(2)+2*2+3) was used to estimate the influence of temperature (17·2-22·8°C) and pH (3·28-4·7) on A. section Nigri growth (λ, lag time; μ, growth rate). S...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04803.x
更新日期:2010-09-01 00:00:00
abstract:AIMS:Monitoring for Salmonella in slaughter pigs is important to enable targeted control measures to be applied on problem farms and at the abattoir. The aim of this study was to determine whether pooled serum and meat juice could be used to identify finishing pig herds with a high prevalence of infection. METHODS AND...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,多中心研究
doi:10.1046/j.1365-2672.2003.02070.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:The main objective of the study is to develop and improve quick bacterial tests to select the best candidates for the bioaugmentation of metal-contaminated soil, coupled with phytoextraction. METHODS AND RESULTS:Bacteria isolates (181) were selected from a collection originated from a Cu-contaminated sediment, on...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12865
更新日期:2015-08-01 00:00:00
abstract:AIM:Assessment of biological control of Pseudomonas syringae pv. aptata using crude lipopeptide extracts (CLEs) of two Bacillus amyloliquefaciens strains (SS-12.6 and SS-38.4) and one Bacillus pumilus strain (SS-10.7). METHODS AND RESULTS:The minimum inhibitory concentration (MIC) of CLEs and their combinations agains...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14070
更新日期:2019-01-01 00:00:00