Abstract:
AIMS:The present work aimed to obtain bioinsecticide over-producing mutants through classical mutagenesis of vegetative cells of Bacillus thuringiensis (Bt) by using u.v. and nitrous acid, and to evidence the involvement of cell metabolism in delta-endotoxin synthesis. METHODS AND RESULTS:Vegetative cells of Bt were treated by nitrous acid (0.17 mg ml(-1)) or exposed to u.v. rays (emitted at a wave length of 240 nm). The isolated survivors were screened on the basis of the production of delta-endotoxins and biomass in glucose and/or in gruel-based media at two aeration conditions. Bioinsecticide over-producing mutants were obtained with high frequencies because random mutations were shown to affect cell metabolism at different pathways related to the regulation of delta-endotoxin synthesis. CONCLUSIONS:Classical mutagenesis of Bt cells lead to the isolation of a large variety of delta-endotoxin over-producing mutants that could be classified into six groups based on the location of the mutations, particularly in metabolism pathways and delta-endotoxin synthesis. SIGNIFICANCE AND IMPACT OF THE STUDY:High frequencies of delta-endotoxin over-producing mutants of Bt could be obtained through classical mutagenesis of vegetative cells. This should contribute to a significant reduction of production and utilization costs of Bt bioinsecticides.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Ghribi D,Zouari N,Jaoua Sdoi
10.1111/j.1365-2672.2004.02323.xsubject
Has Abstractpub_date
2004-01-01 00:00:00pages
338-46issue
2eissn
1364-5072issn
1365-2672pii
JAM2323journal_volume
97pub_type
杂志文章abstract:AIMS:To investigate the role of lipopolysaccharide (LPS) structure in the stability of outer membrane and the ability of biofilm formation in Cronobacter sakazakii. METHODS AND RESULTS:A C. sakazakii mutant strain LWW02 was constructed by inactivating the gene ESA_04107 encoding for heptosyltransferase I. LPS were pur...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05263.x
更新日期:2012-05-01 00:00:00
abstract:AIMS:To avoid interference by water-iodine disinfection chemistry and measure directly the effect of iodine, captured from a triiodide complex bound to a filter medium, on viability of penetrating viral particles. METHODS AND RESULTS:Aerosols of MS2 coli phage were passed through control P100 or iodinated High-Efficie...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12788
更新日期:2015-06-01 00:00:00
abstract:AIMS:Filoviruses are associated with high morbidity and lethality rates in humans, are capable of human-to-human transmission, via infected material such as blood, and are believed to have low infectious doses for humans. Filoviruses are able to infect via the respiratory route and are lethal at very low doses in exper...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04778.x
更新日期:2010-11-01 00:00:00
abstract:AIMS:The minimum inhibitory concentration (MIC) of oregano essential oil (OEO) and two of its principle components, i.e. thymol and carvacrol, against Pseudomonas aeruginosa and Staphylococcus aureus was assessed by using an innovative technique. The mechanism of action of the above substances was also investigated. M...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01428.x
更新日期:2001-09-01 00:00:00
abstract:AIMS:In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. METHODS AND RESULTS:Damage on cell surface was studied by flow cytometry, zeta potential and atomic forc...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12796
更新日期:2015-06-01 00:00:00
abstract:AIMS:Antibiotics and several other chemicals have been used to prevent fish diseases. However, this situation results in economic loss for the companies in the aquaculture industry and most importantly it pollutes the environment. Cinnamic acid is a naturally occurring aromatic acid and is considered to be safe for hum...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14097
更新日期:2018-09-04 00:00:00
abstract:AIM:The main aim of the present study was to use three PCR-based techniques for the analysis of genetic variability among Vibrio parahaemolyticus strains isolated from the Philippines. METHODS AND RESULTS:Seventeen strains of V. parahaemolyticus isolated from shrimps (Penaeus monodon) and from the environments where t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02571.x
更新日期:2005-01-01 00:00:00
abstract::Two polychlorinated biphenyl-contaminated sites in the Czech Republic, a soil at Zamberk and a sediment sludge at Milevsko, were screened for the presence of chlorobenzoate degraders. Sixteen different chlorobenzoate degraders were isolated from the soil compared with only three strains isolated from the sediment. Fro...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00830.x
更新日期:1999-09-01 00:00:00
abstract::A Gram-positive rod was isolated from a commercial grape wine undergoing a sluggish/stuck alcoholic fermentation. The organism produced L-lactic acid from glucose, possessed weak catalase activity, and fermented relatively few carbohydrates, i.e. glucose, fructose, sucrose, raffinose (weakly) and mannitol. The 16S rRN...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00399.x
更新日期:1998-05-01 00:00:00
abstract:AIMS:The aims of the project were threefold: to survey the use of disinfectants in the UK food industry; to assess the product and environmental microflora of selected food factories for the persistence of Listeria monocytogenes and Escherichia coli; and to determine the disinfectant resistance of any persistent strain...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract:AIM:Assessment of biological control of Pseudomonas syringae pv. aptata using crude lipopeptide extracts (CLEs) of two Bacillus amyloliquefaciens strains (SS-12.6 and SS-38.4) and one Bacillus pumilus strain (SS-10.7). METHODS AND RESULTS:The minimum inhibitory concentration (MIC) of CLEs and their combinations agains...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14070
更新日期:2019-01-01 00:00:00
abstract:AIMS:To determine if nisin F-loaded self-setting brushite cement could control the growth of Staphylococcus aureus in vivo. METHODS AND RESULTS: Brushite cement was prepared by mixing equimolar concentrations of β-tricalcium phosphate and monocalcium phosphate monohydrate. Nisin F was added at 5·0, 2·5 and 1·0% (w/w)...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05241.x
更新日期:2012-04-01 00:00:00
abstract::Dental chair units (DCUs) contain integrated systems that provide the instruments and services for a wide range of dental procedures. DCUs use water to cool and irrigate DCU-supplied instruments and tooth surfaces during dental treatment. Water is supplied to these instruments by a network of interconnected narrow-bor...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2672.2008.04100.x
更新日期:2009-05-01 00:00:00
abstract:AIMS:This study evaluated the antibiotic-induced changes in microbial ecology, intestinal dysbiosis and low-grade inflammation; and the combined effect of four different Lactobacillus species on recovery of microbiota composition and improvement of gut barrier function in mice. METHODS AND RESULTS:Administration of th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13687
更新日期:2018-03-01 00:00:00
abstract:AIMS:To identify and examine the diversity of predominant lactic acid bacteria (LAB) in koko and koko sour water (KSW) from different Ghanaian production sites with regard to pattern of fermentation (API 50 CHL), genotype, antimicrobial activity, and resistance to low pH and bile salts. METHODS AND RESULTS:In total 21...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2004.02162.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:The purpose of this work was to study the involvement of micro-organisms, which develop together with Botrytis cinerea on grapes, in the SO2 binding power of musts. METHODS AND RESULTS:Yeasts and bacteria were involved. Most bacteria were acetic acid bacteria, mainly of the Gluconobacter genus. Unlike oxidative y...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01200.x
更新日期:2001-01-01 00:00:00
abstract:AIMS:To determine the mechanisms of Bacillus subtilis spore killing by and resistance to the general biological decontamination agents, Decon and Oxone. METHODS AND RESULTS:Spores of B. subtilis treated with Decon or Oxone did not accumulate DNA damage and were not mutagenized. Spore killing by these agents was increa...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2004.02159.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:The effects of pH, temperature, phytate, glucose, phosphate and surfactants on the phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle, were evaluated. METHOD AND RESULTS:By using batch fermentation, it was found that phytase production of M. jalaludinii was induced by ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01727.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To evaluate an integrated cell culture (ICC)-multiplex-nested PCR using the buffalo green monkey kidney (BGMK) cells for the simultaneous detection of both enteroviruses and adenoviruses in surface water and tap water samples and optimize the procedure for more sensitive detection of virus showing no apparent cyto...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02496.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To analyse genetic diversity and epidemiological relationships among 54 strains of Allorhizobium vitis isolated in Europe during an 8-year period and to assess the relative contribution of mutation and recombination in shaping their diversity. METHODS AND RESULTS:By using random amplified polymorphic DNA (RAPD) P...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12858
更新日期:2015-09-01 00:00:00
abstract:AIMS:To investigate the prevalence, seasonality and genotype distribution of human noroviruses (NoVs) in wastewater in Japan. METHODS AND RESULTS:Influent and effluent water samples were collected monthly for a year from a wastewater treatment plant and examined for the presence of genogroups I and II (GI and GII) NoV...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05231.x
更新日期:2012-03-01 00:00:00
abstract:AIMS:To investigate whether the use of direct viable count (DVC), quantitative viable count (qDVC), colony-forming units and the contribution of capsule-bearing bacteria to the total number of bacteria and esterase-active bacteria could be used to clearly differentiate viable cells in various trophic status of seawater...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04411.x
更新日期:2010-01-01 00:00:00
abstract:AIMS:To employ an in vitro screening regime to select a probiotic Bifidobacterium strain to complement resistant starch (Hi-maizetrade mark) in a synbiotic yoghurt. METHODS AND RESULTS:Of 40 Bifidobacterium isolates examined, only B. lactis Laftitrade mark B94 possessed all of the required characteristics. This isolat...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01240.x
更新日期:2001-02-01 00:00:00
abstract:AIMS:The objective of the present work was to describe an aerobic, mesophilic and heterotrophic marine bacterium, designated HYD657, able to produce an exopolysaccharide (EPS). It was isolated from a East Pacific Rise deep-sea hydrothermal vent polychaete annelid. METHODS AND RESULTS:This micro-organism, on the basis ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01689.x
更新日期:2002-01-01 00:00:00
abstract::Staphylococcus aureus is an important agent of contagious bovine intramammary infections in dairy cattle. Its ability to persist inside the udder is based on the presence of important mechanisms such as its ability to form biofilms, polysaccharide capsules small colony variants, and their ability to invade professiona...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.14706
更新日期:2020-11-01 00:00:00
abstract:AIMS:A novel alginate oligomer (OligoG CF-5/20) has been shown to potentiate antifungal therapy against a range of fungal pathogens. The current study assessed the effect of this oligomer on in vitro virulence factor expression and epithelial invasion by Candida species. METHODS AND RESULTS:Plate substrate assays and ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13516
更新日期:2017-09-01 00:00:00
abstract:AIMS:The study aims to demonstrate the antimicrobial and disinfectant resistance phenotypes and genotypes of Escherichia coli isolates obtained from giant pandas (Ailuropoda melanoleuca). METHODS AND RESULTS:Antimicrobial testing was performed according to the standard disk diffusion method. The minimal inhibitory con...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12820
更新日期:2015-07-01 00:00:00
abstract:AIMS:To characterize atrazine-degrading potential of bacterial communities enriched from agrochemical factory soil by analysing diversity and organization of catabolic genes. METHODS AND RESULTS:The bacterial communities enriched from three different sites of varying atrazine contamination mineralized 65-80% of (14) C...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04700.x
更新日期:2010-07-01 00:00:00
abstract:AIMS:Fungal infection is still a life-threatening risk for the immunocompromised population such as AIDS patients and those who receive treatments with immunosuppressors and/or frequent administrations of wide-spectrum antibiotics, which inevitably lead to the drug resistance and unbalanced microflora populations. The ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02760.x
更新日期:2006-01-01 00:00:00
abstract:AIMS:To establish the microbiological quality of cold ready-to-eat sliced meats and pâté from catering and retail premises, and investigate links hypothesized between foodborne Campylobacter infection and the consumption of cold sliced meats. METHODS AND RESULTS:A total of 4078 cold meat and pâté samples were collecte...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02203.x
更新日期:2004-01-01 00:00:00