The simultaneous detection of both enteroviruses and adenoviruses in environmental water samples including tap water with an integrated cell culture-multiplex-nested PCR procedure.

abstract：AIMS:Cloning and expression of a new cry1Ia-type gene of Bacillus thuringiensis. METHODS AND RESULTS:PCR amplification, using gene cry1I-specific primers revealed the presence of such a gene in the strain BNS3 of Bacillus thuringiensis subsp. kurstaki. The cloning and sequencing from BNS3 of the cry1Ia-type gene, call...

journal_title：Journal of applied microbiology

authors： Tounsi S,Zouari N,Jaoua S

更新日期：2003-01-01 00:00:00

abstract：AIMS:To better understand and manage the fate and transport of Salmonella in agricultural watersheds, we developed a culture-based, five tube-four dilution most probable number (MPN) method for enumerating dilute densities of Salmonella in environmental waters. METHODS AND RESULTS:The MPN method was a combination of a...

journal_title：Journal of applied microbiology

authors： Jenkins MB,Endale DM,Fisher DS

更新日期：2008-06-01 00:00:00

abstract：AIMS:This study aimed at characterizing the lactic acid bacteria microbiota and selecting mixed endogenous starters to be used for sourdough fermentation of spelt or emmer flours. METHODS AND RESULTS:Identification of lactic acid bacteria was carried out by partial sequencing of the 16S rRNA, recA, 16S/23S rRNA spacer...

journal_title：Journal of applied microbiology

authors： Coda R,Nionelli L,Rizzello CG,De Angelis M,Tossut P,Gobbetti M

更新日期：2010-03-01 00:00:00

abstract：AIMS:Develop an effective laboratory method to consistently recover viral loads from porous concrete coupons sufficient for disinfectant efficacy testing. Investigate the role of concrete matrix pH on the recovery of foot-and-mouth disease virus (FMDV) and African Swine Fever virus (ASFV) from porous concrete. Compare ...

journal_title：Journal of applied microbiology

authors： Gabbert LR,Neilan JG,Rasmussen M

更新日期：2020-11-01 00:00:00

abstract：AIMS:To determine the prevalence of Arcobacter in various food, animal and water sources in Turkey and to subtype the isolated strains using enterobacterial repetitive intergenic consensus (ERIC)-PCR. METHODS AND RESULTS:A total of 806 samples consisting of chicken (100) and turkey meat (100); minced beef (27); rectal...

journal_title：Journal of applied microbiology

authors： Aydin F,Gümüşsoy KS,Atabay HI,Iça T,Abay S

更新日期：2007-07-01 00:00:00

abstract：AIMS:The study set out to sample € 10 banknotes for the presence of coagulase-positive staphylococci (CPS) such as Staphylococcus aureus (S. aureus) and coagulase-negative staphylococci (CoNS) in Southern Ireland, to assess the levels of antibiotic resistance among those isolated, and determine the persistence of S. au...

journal_title：Journal of applied microbiology

authors： Gabriel EM,Coffey A,O'Mahony JM

更新日期：2013-08-01 00:00:00

abstract：AIMS:Bacillus licheniformis PWD-1 is a keratin-degrading, spore-forming bacterium isolated from a poultry waste digester. A sporulation-deficient mutant of B. licheniformis PWD-1, named B. licheniformis WBG, was developed and characterized. METHODS AND RESULTS:The mutation was generated using the splicing by overlap e...

journal_title：Journal of applied microbiology

authors： Wang JJ,Greenhut WB,Shih JC

更新日期：2005-01-01 00:00:00

abstract：AIMS:The aim of this work was to investigate the effects of biodynamic management with and without the addition of green manure, in comparison with organic management, on the microbiota in vineyards soil. METHODS AND RESULTS:High throughput sequencing was used to compare the taxonomic structure of the soil bacterial a...

journal_title：Journal of applied microbiology

authors： Longa CMO,Nicola L,Antonielli L,Mescalchin E,Zanzotti R,Turco E,Pertot I

更新日期：2017-12-01 00:00:00

abstract：AIMS:Lactobacilli are widely distributed in food and the environment, and some colonize the human body as commensal bacteria. The aim of this study was to determine the species of lactobacilli that colonize the vagina and compare them with those found in food and the environment. METHODS AND RESULTS:Thirty-five Lactob...

journal_title：Journal of applied microbiology

authors： Pavlova SI,Kilic AO,Kilic SS,So JS,Nader-Macias ME,Simoes JA,Tao L

更新日期：2002-01-01 00:00:00

abstract：AIM:The study was carried out to assess the agreement of API 50 CH fermentation data of food lactobacilli with their RAPD profiles to determine whether the system could be used alone as a reliable taxonomic tool for this genus. METHODS AND RESULTS:API 50 CH, RAPD and DNA:DNA reassociation data for 42 lactobacilli from...

journal_title：Journal of applied microbiology

authors： Nigatu A

更新日期：2000-12-01 00:00:00

abstract：AIMS:To study the marked resistance of Periplaneta americana to entomopathogenic Metarhizium anisopliae. METHODS AND RESULTS:The low susceptibility of 4th instar nymphs applied topically with conidia seemed to be related to an active removal of conidia by the cockroach and to a disabled or retarded germination and sub...

journal_title：Journal of applied microbiology

authors： Gutierrez AC,Machado JA,Hubner-Campos R,Pennisi MA,Rodrigues J,López Lástra CC,García JJ,Fernandes ÉK,Luz C

更新日期：2016-11-01 00:00:00

abstract：:The rumen contents of red deer (Cervus elaphus) were used to isolate bacterial capable of fermenting glycerol. The biochemistry, physiology, morphology and phylogeny of one isolate were studied in detail. The isolate (DR3) was tentatively identified as a strain of the species Klebsiella planticola as based on phenotyp...

journal_title：Journal of applied microbiology

authors： Jarvis GN,Moore ER,Thiele JH

更新日期：1997-08-01 00:00:00

abstract：AIMS:To assess the presence of virulence genes in environmental and foodborne Escherichia coli isolates using the TaqMan PCR system. METHODS AND RESULTS:Three TaqMan pathogen detection kits called O157:H7, StxI and StxII were used to investigate the presence of virulence genes in Escherichia coli isolates. All 54 food...

journal_title：Journal of applied microbiology

authors： Davis KC,Nakatsu CH,Turco R,Weagant SD,Bhunia AK

更新日期：2003-01-01 00:00:00

abstract：AIMS:To determine the effect of antimicrobial selective pressure on the transmission of antimicrobial resistant and sensitive strains of Salmonella in poultry. METHODS AND RESULTS:Eight pens housed 12 broiler chicks each. Two chicks in four of the pens were inoculated with a Salm. Typhimurium strain resistant to 12 an...

journal_title：Journal of applied microbiology

authors： Bauer-Garland J,Frye JG,Gray JT,Berrang ME,Harrison MA,Fedorka-Cray PJ

更新日期：2006-12-01 00:00:00

abstract：AIM:To evaluate the bacterial composition of collared peccary semen and foreskin mucosa, and to verify the sensitivity of isolates to antimicrobials used in semen conservation and to Aloe vera gel, which is an alternative external cryoprotectant. METHODS AND RESULTS:Nine foreskin mucosa and ejaculate samples from adul...

journal_title：Journal of applied microbiology

authors： Santos CS,Silva AM,Maia KM,Rodrigues GSO,Feijó FMC,Alves ND,Oliveira MF,Silva AR

更新日期：2020-09-01 00:00:00

abstract：:A novel antibacterial substance produced by a strain isolated from Bulgarian yellow cheese was characterized. The producer strain was identified by molecular typing to belong to the species Lactobacillus delbrueckii, which is a rare producer of bacteriocins. The inhibitory agent was heat stable and active against lact...

journal_title：Journal of applied microbiology

authors： Miteva V,Ivanova I,Budakov I,Pantev A,Stefanova T,Danova S,Moncheva P,Mitev V,Dousset X,Boyaval P

更新日期：1998-09-01 00:00:00

abstract：AIMS:Evaluation of the antibacterial activity of cultivable bacteria associated with the marine sponges Hymeniacidon perlevis and Halichondria panicea against multi-drug-resistant Staphylococcus aureus. METHODS AND RESULTS:One hundred and fourteen bacterial isolates were recovered from H. perlevis and H. panicea. Anti...

journal_title：Journal of applied microbiology

authors： Rodriguez Jimenez A,Dechamps E,Giaux A,Goetghebuer L,Bauwens M,Willenz P,Flahaut S,Laport MS,George IF

更新日期：2021-01-09 00:00:00

abstract：AIMS:This study evaluated the microbiological quality and safety of minimally processed parsley sold in southeastern Brazilian food markets. METHODS AND RESULTS:One hundred samples were submitted to the enumeration of Enterobacteriaceae by plating on MacConkey agar. Colonies of Enterobacteriaceae were randomly selecte...

journal_title：Journal of applied microbiology

authors： Finger JAFF,Maffei DF,Dias M,Mendes MA,Pinto UM

更新日期：2020-11-19 00:00:00

abstract：AIMS:Strains of Verocytotoxin-producing Escherichia coli (VTEC) from Scottish beef cattle on the same farm were isolated during four visits over a period of eight months. Characteristics of these strains were examined to allow comparisons with strains of VTEC associated with human infection. METHODS AND RESULTS:Strain...

journal_title：Journal of applied microbiology

authors： Jenkins C,Pearce MC,Chart H,Cheasty T,Willshaw GA,Gunn GJ,Dougan G,Smith HR,Synge BA,Frankel G

更新日期：2002-01-01 00:00:00

abstract：AIMS:Plasmid profile, phage typing, and pulsed-field gel electrophoresis (PFGE) patterns of 124 Salmonella Enteritidis strains isolated in 1998-2002 in Taiwan were analysed and the results were compared with those of the 63 strains obtained in 1991-1997, so that molecular subtypes and epidemic strains for Salmonella En...

journal_title：Journal of applied microbiology

authors： Pang JC,Chiu TH,Chiou CS,Schroeter A,Guerra B,Helmuth R,Tsen HY

更新日期：2005-01-01 00:00:00

abstract：AIMS:  The effect of refrigeration on the seafood-borne pathogen Vibrio vulnificus was investigated in terms of genotype selection and persistence in refrigerated oysters. METHODS AND RESULTS:  Naturally occurring numbers of V. vulnificus in oysters from two different locations were compared during a 2-week period und...

journal_title：Journal of applied microbiology

authors： Wood RR,Arias CR

更新日期：2012-07-01 00:00:00

abstract：AIMS:To determine the effect of solar radiation on Cryptosporidium parvum in tap and environmental waters. METHODS AND RESULTS:Outdoor tank experiments and a cell culture infectivity assay were used to measure solar inactivation of C. parvum oocysts in different waters. Experiments conducted on days with different lev...

journal_title：Journal of applied microbiology

authors： King BJ,Hoefel D,Daminato DP,Fanok S,Monis PT

更新日期：2008-05-01 00:00:00

abstract：AIMS:To determine the transcriptional responses of Trichophyton rubrum to the artificial substance, PH11B. METHODS AND RESULTS:The broth microdilution assay for antifungal susceptibility testing of dermatophytes was used to measure the minimum inhibitory concentration (MIC) of PH11B. cDNA microarray technology and rea...

journal_title：Journal of applied microbiology

authors： Yu L,Zhang W,Liu T,Wang X,Peng J,Li S,Jin Q

更新日期：2007-12-01 00:00:00

abstract：:The volatile oils of black pepper [Piper nigrum L. (Piperaceae)], clove [Syzygium aromaticum (L.) Merr. & Perry (Myrtaceae)], geranium [Pelargonium graveolens L'Herit (Geraniaceae)], nutmeg [Myristica fragrans Houtt. (Myristicaceae), oregano [Origanum vulgare ssp. hirtum (Link) Letsw. (Lamiaceae)] and thyme [Thymus vu...

journal_title：Journal of applied microbiology

authors： Dorman HJ,Deans SG

更新日期：2000-02-01 00:00:00

abstract：AIMS:Enzyme substrates based on 4-methylumbelliferone are widely used for the detection of Escherichia coli and enterococci in water, by detection of beta-glucuronidase and beta-glucosidase activity respectively. This study aimed to synthesize and evaluate novel umbelliferone-based substrates with improved sensitivity ...

journal_title：Journal of applied microbiology

authors： Perry JD,James AL,Morris KA,Oliver M,Chilvers KF,Reed RH,Gould FK

更新日期：2006-11-01 00:00:00

abstract：AIM:Calibration of impedance measurement was performed vs the Association Françoise de Normalisation (AFNOR) MPN method with a view to rapid enumeration of Escherichia coli in live marine bivalve molluscs. METHODS AND RESULTS:Linear regression models between log10 MPN and detection time (DT) were adjusted for several ...

journal_title：Journal of applied microbiology

authors： Dupont J,Dumont F,Menanteau C,Pommepuy M

更新日期：2004-01-01 00:00:00

abstract：AIMS:The effects of freeze-drying, spray-drying and fluidized bed-drying on survival of Epicoccum nigrum conidia were compared. METHODS AND RESULTS:Viability of E. nigrum conidia (estimated by measuring its germination) was 100% after fluidized bed-drying and freeze-drying, but it was determined that skimmed milk must...

journal_title：Journal of applied microbiology

authors： Larena I,De Cal A,Liñán M,Melgarejo P

更新日期：2003-01-01 00:00:00

abstract：AIMS:To study the accumulation of the bacterial living cells (LC) and dead cells (DC) in a mixed-species biofilm developed in a 3 l biotrickling filter (BTF) challenged with toluene. METHODS AND RESULTS:The bacterial LC and DC within the biofilm developed on polypropylene Pall rings in a toluene-degrading BTF were enu...

journal_title：Journal of applied microbiology

authors： Tresse O,Lescob S,Rho D

更新日期：2003-01-01 00:00:00

abstract：:Rapid enumeration methods based on the enzymatic hydrolysis of 4-methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide were optimized for freshwaters. The enzymes beta-D-galactosidase (GALase) and beta-D-glucuronidase (GLUase) were shown to be already induced in freshwaters when tested, res...

journal_title：Journal of applied microbiology

authors： George I,Petit M,Servais P

更新日期：2000-03-01 00:00:00

abstract：AIMS:To investigate the involvement of oxidative stress and thiol redox state (TRS) in sclerotial differentiation of Sclerotium rolfsii and Sclerotinia sclerotiorum. METHODS AND RESULTS:Oxidative stress in these fungi was assessed by lipid peroxidation, which was higher in comparison with their nonsclerotiogenic count...

journal_title：Journal of applied microbiology

authors： Patsoukis N,Georgiou CD

更新日期：2008-01-01 00:00:00