Abstract:
AIMS:This study evaluated the microbiological quality and safety of minimally processed parsley sold in southeastern Brazilian food markets. METHODS AND RESULTS:One hundred samples were submitted to the enumeration of Enterobacteriaceae by plating on MacConkey agar. Colonies of Enterobacteriaceae were randomly selected and identified by MALDI-TOF MS. Samples were also tested for Listeria monocytogenes and Salmonella sp. The mean count of Enterobacteriaceae was 6·0 ± 1·0 log CFU per gram, while 18 genera (including 30 species) of bacteria belonging to this family were identified. Salmonella and L. monocytogenes were not detected, while L. innocua was found in two samples and L. fleischmannii was found in one sample. Moreover generic Escherichia coli was found in three samples, all from different brands of minimally processed parsley. CONCLUSIONS:Even though microbial pathogens were not isolated, a variety of indicator micro-organisms were identified, including vegetable spoilers and species capable of causing human opportunistic infections. These results suggest hygienic failures and/or lack of temperature control during processing and storage of these ready-to-eat products. SIGNIFICANCE AND IMPACT OF STUDY:This study highlights the need for control measures during the production chain of minimally processed parsley in order to reduce microbial contamination and the risks of foodborne diseases.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Finger JAFF,Maffei DF,Dias M,Mendes MA,Pinto UMdoi
10.1111/jam.14935subject
Has Abstractpub_date
2020-11-19 00:00:00eissn
1364-5072issn
1365-2672pub_type
杂志文章abstract:AIMS:To identify active phenolic constituents in muscadine grape skin (MGS) extracts and determine interactions among compounds while further exploring their anti-Helicobacter pylori potential in vitro. METHODS AND RESULTS:The inhibitory effects of quercetin and resveratrol, active polyphenols identified in MGS extrac...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12129
更新日期:2013-04-01 00:00:00
abstract::Heterotrophic plate count (HPC) bacteria are naturally present in all aqueous environments. These bacteria undergo multiplication cycles in drinking water, especially in closed containers (bottled water) or in tap water when chlorine levels are dissipated, such as in dead ends in water mains or household plumbing. A s...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00134.x
更新日期:1997-04-01 00:00:00
abstract:AIMS:To obtain the high-yield strain of fusidic acid, which is produced from fungus Fusidium coccineum and is the only fusidane-type antibiotic that has been used clinically, and confirm the changes in the transcription levels involved in increasing its production. METHODS AND RESULTS:By using the atmospheric and room...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14797
更新日期:2020-07-30 00:00:00
abstract:AIMS:This study aimed at characterizing the lactic acid bacteria microbiota and selecting mixed endogenous starters to be used for sourdough fermentation of spelt or emmer flours. METHODS AND RESULTS:Identification of lactic acid bacteria was carried out by partial sequencing of the 16S rRNA, recA, 16S/23S rRNA spacer...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04497.x
更新日期:2010-03-01 00:00:00
abstract:AIMS:The aim of the study was to check whether two flavanols ((-)-epigallocatechin gallate and (+)-catechin) can ameliorate oxidative stress (OS) accompanying and contributing to the lethal effects of heating (50°C) and freezing-thawing on the yeast Saccharomyces cerevisiae. METHODS AND RESULTS:The flavanols studied i...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14170
更新日期:2019-03-01 00:00:00
abstract::Thirty isolates of Listeria monocytogenes and 18 of L. innocua obtained from different short-ripened cheeses manufactured in Asturias (northern Spain), were compared with each other and with reference strains using serotype, phage type and pulsed-field restriction endonuclease digestion profiles analysis of the total ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00339.x
更新日期:1998-02-01 00:00:00
abstract:AIM:To understand the underlying cellular mechanisms during inactivation of Escherichia coli in response to antimicrobial solution of nonthermal plasma-activated N-acetylcysteine (NAC). METHODS AND RESULTS:The recommended techniques were used to demonstrate E. coli cellular and transcriptomic changes caused associated...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13777
更新日期:2018-08-01 00:00:00
abstract:AIMS:Develop an effective laboratory method to consistently recover viral loads from porous concrete coupons sufficient for disinfectant efficacy testing. Investigate the role of concrete matrix pH on the recovery of foot-and-mouth disease virus (FMDV) and African Swine Fever virus (ASFV) from porous concrete. Compare ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14694
更新日期:2020-11-01 00:00:00
abstract:AIM:To study the microbiology of intensive, in-vessel biodegradation of a mixture of sewage sludge and vegetable food waste. METHODS AND RESULTS:The biodegradation was performed in a closed reactor with the addition of a starter culture of Bacillus thermoamylovorans SW25 under conditions of controlled aeration, stirri...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02182.x
更新日期:2004-01-01 00:00:00
abstract:AIM:Nano-biotechnologically synthesizing silver nanoparticles via canthaxanthin pigment extracted from Dietzia maris AURCCBT01 and assessing their cytotoxic therapeutic potential against human keratinocyte cell line (HaCaT) were the key objectives of this study. METHODS AND RESULTS:The pigment extracted from D. maris ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14889
更新日期:2020-10-08 00:00:00
abstract:AIM:To investigate the effect of corn starch (CS) edible films with spice oils on the stability of raw beef during refrigerated storage. METHODS AND RESULTS:The antimicrobial properties of corn starch films containing 0-4·0% (w/v) ratios of clove (CL) and cinnamon (CI) essential oils (EOs) were tested against seven me...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12932
更新日期:2015-12-01 00:00:00
abstract:AIMS:To determine the reliable combination of protocols for specific detection and identification of R. solanacearum race 3 biovar 2 (R3bv2) through a comprehensive comparison among currently available techniques. METHODS AND RESULTS:Sensitivity and specificity of the conventional isolation, bioassay, serological assa...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12585
更新日期:2014-10-01 00:00:00
abstract:AIMS:The purpose of this study was to comprehensively compare the response of nine biosensors capable of being induced by Hg. Induction by Hg was based upon the insertion of merR, merB, zntA and zntR promoter genes. LuxCDABE or lucFF reporter genes expressed luminescence, and host organisms were Escherichia coli, Vibri...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02421.x
更新日期:2004-01-01 00:00:00
abstract::Pathogens express virulence genes in response to the combination of environmental conditions present in the host environment. The crop is the first gastrointestinal environment encountered in birds. However, feed withdrawal alters the crop environment resulting in an increased pH, and decreased concentrations of lacta...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01089.x
更新日期:2000-07-01 00:00:00
abstract:AIMS:The aim of this study was to examine the contribution of a five-gene islet (lmo0444 - lmo0448) to the growth of Listeria monocytogenes under suboptimal conditions. METHODS AND RESULTS:Bioinformatics and PCR analyses revealed that a five-gene islet is present in c. half of all L. monocytogenes strains examined (66...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04726.x
更新日期:2010-09-01 00:00:00
abstract:AIMS:Adenosine triphosphate (ATP) during the enzymatic production of glutathione is necessary. In this study, our aims were to investigate the reason for low glutathione production in Escherichia coli coupled with an ATP regeneration system and to develop a new strategy to improve the system. METHODS AND RESULTS:Gluta...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03415.x
更新日期:2008-02-01 00:00:00
abstract:AIMS:The objective of this study was to determine antimicrobial activities of essential oils (EOs) against bovine respiratory disease (BRD) pathogens and nasopharyngeal commensal bacteria, as well as cytotoxicity in bovine turbinate (BT) cells in vitro. METHODS AND RESULTS:The chemical composition of 16 EOs was determ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14238
更新日期:2019-06-01 00:00:00
abstract::Probiotics are increasingly being added to food in order to develop products with health-promoting properties. Particularly, Saccharomyces cereviceae var. boulardii yeast is recently being investigated like a starting-culture for development of functional and probiotic foods. Although the literature is abundant on the...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.14037
更新日期:2018-10-01 00:00:00
abstract:AIMS:This study elucidates the mechanisms by which a nonbacteriocinogenic Carnobacterium piscicola inhibits growth of Listeria monocytogenes. METHODS AND RESULTS:Listeria monocytogenes was exposed to live cultures of a bacteriocin-negative variant of C. piscicola A9b in co-culture, in a diffusion chamber system, and t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02438.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To investigate the in vivo effects of Lactobacillus rhamnosus GG (LGG) on intestinal polyp development and the interaction between this single-organism probiotic and the gut microbiota therein. METHODS AND RESULTS:The ApcMin/+ mouse model was used to study the potential preventive effect of LGG on intestinal poly...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13386
更新日期:2017-03-01 00:00:00
abstract:AIMS:To determine whether isolates of Listeria monocytogenes differ in their ability to adsorb and form biofilms on a food-grade stainless steel surface. METHODS AND RESULTS:Strains were assessed for their ability to adsorb to a test surface over a short time period. Although some differences in numbers of bound cells...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01419.x
更新日期:2001-10-01 00:00:00
abstract:AIMS:The purpose of this study was to develop a laboratory biofilm growth reactor system that simulated the toilet bowl environment and which could be used for biocide efficacy testing. METHODS AND RESULTS:A microbial biofilm reactor system incorporating intermittent flow and nutrient provision was designed. The react...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01342.x
更新日期:2001-07-01 00:00:00
abstract::The effect of electroporation (very short duration pulses of high voltage electricity) on the viability of Giardia cysts and Cryptosporidium oocysts, and on the viability of these organisms in the presence of free chlorine, combined chlorine, hydrogen peroxide and potassium permanganate, was examined. While electropor...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00725.x
更新日期:1999-06-01 00:00:00
abstract:AIMS:The ComPath project is a pan-European programme dedicated to the monitoring of antimicrobial susceptibility of pathogens from diseased dogs and cats using standardized methods and centralized minimum inhibitory concentration (MIC) determination. Here, the susceptibility of major pathogens is reported from antimicr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13287
更新日期:2016-11-01 00:00:00
abstract:AIMS:To investigate the influence of micro-organisms associated with copper corrosion on 'blue water' corrosion in drinking water. METHODS AND RESULTS:Laboratory rigs comprising of polycarbonate containers attached to annealed copper plumbing tubes were filled with Melbourne drinking water and sterilized by autoclavin...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02341.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:A high-quality inoculum of Gluconacetobacter xylinus is important to produce bacterial cellulose (BC), a versatile biomaterial. This work aims to develop a method of preparing an inoculum of this bacterium with high cell density and without mutants. METHODS AND RESULTS:Inocula of G. xylinus ACCC 10220 without and...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13193
更新日期:2016-09-01 00:00:00
abstract:AIM:To identify and compare lactic acid bacteria (LAB) isolated from alkaline fermentations of cassava (Manihot esculenta Crantz) leaves, roselle (Hibiscus sabdariffa) and African locust bean (Parkia biglobosa) seeds for production of, respectively, Ntoba Mbodi, Bikalga and Soumbala. METHODS AND RESULTS:A total of 121...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04603.x
更新日期:2010-06-01 00:00:00
abstract::Microbial succession during a laboratory-scale composting process of garbage was analysed by denaturing gradient gel electrophoresis (DGGE) combined with measurement of physicochemical parameters such as temperature, pH, organic acids, total dissolved organic carbon and water-soluble humic substance. From the temperat...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01177.x
更新日期:2000-11-01 00:00:00
abstract:AIM:Gonorrhoea remains a leading public health burden and the development of vaccine against gonorrhoea becomes more urgent. Here, a novel Neisseria gonorrhoeae DNA vaccine delivered by Salmonella enteritidis ghosts was developed and the immune responses of the vaccine candidate were evaluated. METHODS AND RESULTS:Nei...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14815
更新日期:2020-08-08 00:00:00
abstract::Sorbitol fermenting bifidobacteria were evaluated as indicators of non-point source human faecal pollution to three sub-estuaries with elevated faecal coliform densities. Human-specific bifidobacteria correlated with identifiable human sanitary deficiencies in feeder streams to estuarine creeks in two of three watersh...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00845.x
更新日期:1999-10-01 00:00:00