Abstract:
AIMS:To investigate the in vivo effects of Lactobacillus rhamnosus GG (LGG) on intestinal polyp development and the interaction between this single-organism probiotic and the gut microbiota therein. METHODS AND RESULTS:The ApcMin/+ mouse model was used to study the potential preventive effect of LGG on intestinal polyposis, while shotgun metagenomic sequencing was employed to characterize both taxonomic and functional changes within the gut microbial community. We found that the progression of intestinal polyps in the control group altered the community functional profile remarkably despite small variation in the taxonomic diversity. In comparison, the consumption of LGG helped maintain the overall functional potential and taxonomic profile in the resident microbes, thereby leading to a 25% decrease of total polyp counts. Furthermore, we found that LGG enriched those microbes or microbial activities related to short-chain fatty acid production (e.g. Roseburia and Coprococcus), as well as suppressed the ones that can lead to inflammation (e.g. Bilophila wadsworthia). CONCLUSIONS:Our study using shotgun metagenomics highlights how single probiotic LGG may exert its beneficial effects and decrease polyp formation in mice by maintaining gut microbial functionality. SIGNIFICANCE AND IMPACT OF THE STUDY:This probiotic intervention targeting microbiota may be used in conjugation with other dietary supplements or drugs as part of prevention strategies for early-stage colon cancer, after further clinical validations in human.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Ni Y,Wong VH,Tai WC,Li J,Wong WY,Lee MM,Fong FL,El-Nezami H,Panagiotou Gdoi
10.1111/jam.13386subject
Has Abstractpub_date
2017-03-01 00:00:00pages
770-784issue
3eissn
1364-5072issn
1365-2672journal_volume
122pub_type
杂志文章abstract:AIMS:To isolate an iron-reducing bacterium and examine its ability of Fe(III) oxide reduction and dechlorination. METHODS AND RESULTS:A fermentative facultative anaerobe, strain L17 isolated from subterranean sediment, can reduce Fe(III) oxides and carbon tetrachloride (CT). It was identified as Klebsiella pneumoniae ...
journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:1998-02-01 00:00:00
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02306.x
更新日期:2004-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14394
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03159.x
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journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2005.02741.x
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02472.x
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journal_title:Journal of applied microbiology
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更新日期:2003-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2021-01-01 00:00:00
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更新日期:2015-09-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02480.x
更新日期:2005-01-01 00:00:00
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2004.02212.x
更新日期:2004-01-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2001-08-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2019-03-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2007-06-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2017-05-01 00:00:00