Abstract:
AIMS:Glycyrrhiza glabra is a high-value medicinal plant thriving in biodiversity rich Kashmir Himalaya. The present study was designed to explore the fungal endophytes from G. glabra as a source of bioactive molecules. METHODS AND RESULTS:The extracts prepared from the isolated endophytes were evaluated for anti-microbial activities using broth micro-dilution assay. The endophytic strain coded as A2 exhibiting promising anti-bacterial as well as anti-tuberculosis activity was identified as Fusarium solani by ITS-5.8S ribosomal gene sequencing technique. This strain was subjected to large-scale fermentation followed by isolation of its bioactive compounds using column chromatography. From the results of spectral data analysis and comparison with literature, the molecules were identified as 3,6,9-trihydroxy-7-methoxy-4,4-dimethyl-3,4-dihydro-1H-benzo[g]isochromene-5,10-dione (1), fusarubin (2), 3-O-methylfusarubin (3) and javanicin (4). Compound 1 is reported for the first time from this strain. All the four compounds inhibited the growth of various tested bacterial strains with MIC values in the range of <1 to 256 μg ml-1 . Fusarubin showed good activity against Mycobacterium tuberculosis strain H37Rv with MIC value of 8 μg ml-1 , whereas compounds 1, 3 and 4 exhibited moderate activity with MIC values of 256, 64, 32 μg ml-1 , respectively. CONCLUSIONS:To the best of our knowledge, this is the first study that reports significant anti-tuberculosis potential of bioactive molecules from endophytic F. solani evaluated against the virulent strain of M. tuberculosis. This study sets background towards their synthetic intervention for activity enhancement experiments in anti-microbial drug discovery programme. SIGNIFICANCE AND IMPACT OF THE STUDY:Due to the chemoprofile variation of same endophyte with respect to source plant and ecoregions, further studies are required to explore endophytes of medicinal plants of all unusual biodiversity rich ecoregions for important and or novel bioactive molecules.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Shah A,Rather MA,Hassan QP,Aga MA,Mushtaq S,Shah AM,Hussain A,Baba SA,Ahmad Zdoi
10.1111/jam.13410subject
Has Abstractpub_date
2017-05-01 00:00:00pages
1168-1176issue
5eissn
1364-5072issn
1365-2672journal_volume
122pub_type
杂志文章abstract:AIMS:Our main interest is to check if programmed cell death (PCD) can occur in prokaryotic algae and if the morphological and biochemical features of PCD are conserved. METHODS AND RESULTS:Using TUNEL labelling, fluorescence and light microscopy and DNA gel electrophoresis, we found that cell death with features simil...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01651.x
更新日期:2002-01-01 00:00:00
abstract:AIM:To investigate the effect of corn starch (CS) edible films with spice oils on the stability of raw beef during refrigerated storage. METHODS AND RESULTS:The antimicrobial properties of corn starch films containing 0-4·0% (w/v) ratios of clove (CL) and cinnamon (CI) essential oils (EOs) were tested against seven me...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12932
更新日期:2015-12-01 00:00:00
abstract:AIMS:A number of previous studies have shown that plant extracts can inhibit formation of dental plaque. The ability of extracts of Rosmarinus officianalis L., Salvia officianalis L., unfermented cocoa, red grape seed and green tea to inhibit plaque bacteria, glucosyltransferase activity, glucan and plaque formation in...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05380.x
更新日期:2012-10-01 00:00:00
abstract:AIMS:This work focuses on the development of a method for the identification of pathogenic yeast. With this aim, we target the nucleotide sequence of the RPS0 gene of pathogenic yeast species with specific PCR primers. PCR analysis was performed with both the genomic DNA, whole cells of clinical isolates of Candida spe...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04595.x
更新日期:2010-06-01 00:00:00
abstract:AIM:To determine the composition and diversity of the microbiota associated to Crassostrea sikamea treated during 30 days with Streptomyces strains N7 and RL8. METHODS AND RESULTS:DNA was extracted from oysters followed by 16S rRNA gene amplification and pyrosequencing. The highest and lowest species diversity richnes...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13382
更新日期:2017-03-01 00:00:00
abstract:AIMS:To repurpose a silver-based antimicrobial textile coating product (Micro-Fresh 1911) as a dual-function antimicrobial laundry additive and textile coating. METHODS AND RESULTS:Survival of Escherichia coli or Staphylococcus aureus type and clinical isolates in a domestic 40°C wash was assessed with and without soi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14850
更新日期:2020-09-11 00:00:00
abstract:AIMS:To investigate the bactericidal activity of lactoferrin-derived peptides and a new LF-derived peptides chimera (LFchimera) against P. aeruginosa and the influence on virulence factors of P. aeruginosa. METHODS AND RESULTS:Lactoferricin (LFcin) and lactoferrampin (LFampin) are highly bioactive peptides isolated fr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04751.x
更新日期:2010-10-01 00:00:00
abstract::NaCl-tolerant lactic acid bacteria (LAB) strains LC-10 (Lactobacillus casei) and LP-15 (Lact. plantarum) and NaCl were used as additives to sorghun (Sorghum bicolor). Numbers of LAB were significantly (P < 0.05) higher in all the additive-treated silages than in the control silage at an early stage of ensiling. During...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00229.x
更新日期:1997-09-01 00:00:00
abstract:AIMS:To predict and validate survival of non-acid adapted Escherichia coli O157 in an environment mimicking the human stomach. METHODS AND RESULTS:Survival was predicted mathematically from inactivation rates at various, but constant pH values. Predictions were subsequently validated experimentally in a pH-controlled ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01865.x
更新日期:2003-01-01 00:00:00
abstract::Raw milk from 27 farms was sampled over 6 months for listerias, salmonellas, Yersinia enterocolitica and campylobacters. Total bacterial counts and somatic cell counts were measured. Lactococci, lactobacilli, dextran-producing leuconostocs, Brevibacterium linens, yeasts and moulds, Staphylococcus aureus and other Micr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00166.x
更新日期:1997-07-01 00:00:00
abstract:AIMS:Lactobacilli are widely distributed in food and the environment, and some colonize the human body as commensal bacteria. The aim of this study was to determine the species of lactobacilli that colonize the vagina and compare them with those found in food and the environment. METHODS AND RESULTS:Thirty-five Lactob...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01547.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:Microbial induced calcium carbonate precipitation (MICP) is one of the bio-cementation methods for improving granular soils. This study evaluate the feasibility of obtaining a bacterial solution with high optical density and urease activity by an inexpensive corn steep liquor (CSL) medium in non-sterile conditions...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14866
更新日期:2020-10-07 00:00:00
abstract:AIMS:To develop qPCR broad-spectrum primers combined with a Nocardia genus-specific probe for the identification of a broad spectrum of Nocardia spp. and to analyse the effects of using this developed primer and probe set on the ability to quantify Nocardia spp. in mixed DNA. METHODS AND RESULTS:The consequences of us...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13311
更新日期:2017-01-01 00:00:00
abstract:AIMS:The aim of this study was to isolate and identify bacterial strains capable of using recalcitrant compounds of molasses spent wash as sole carbon source from the soils of abandoned sites of distillery effluent discharge and characterize their ability of reducing the chemical oxygen demand (COD) of the spent wash. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02289.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To investigate the interactions of Salmonella enterica with abiotic and plant surfaces and their effect on the tolerance of the pathogen to various stressors. METHODS AND RESULTS:Salmonella strains were tested for their ability to form biofilm in various growth media using a polystyrene plate model. Strong biofil...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04152.x
更新日期:2009-06-01 00:00:00
abstract:AIMS:Silage is grass, preserved by fermentation and used as winter feed for cattle. The impact of a range of current grass silage preparation practices on the survival of Escherichia coli C600φ3538(Δvtx2 ::cat) and on the induction, release and infectivity of free phage were investigated. METHODS AND RESULTS:Wilted an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13431
更新日期:2017-05-01 00:00:00
abstract:AIMS:To evaluate archaeal diversity in natural and impacted habitats from Rio de Janeiro state, Brazil, a tropical region of South America. METHODS AND RESULTS:16S rRNA gene was amplified directly by polymerase chain reaction (PCR) from genomic DNA, extracted from Guanabara Bay (GB) water, halomarine sediment (HS), mu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03230.x
更新日期:2007-07-01 00:00:00
abstract:AIM:To assess the role of lactate as a precursor for butyrate biosynthesis in human colonic microflora. METHODS AND RESULTS:Three human faecal microfloras were incubated in vitro with media supplemented with 30 mmol l(-1) unenriched or 13C-enriched lactate. Lactate metabolism and short-chain fatty acid (SCFA) producti...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02605.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To identify the prevalence and types of Campylobacter jejuni carried by dairy cattle and the extent of overlap of these types with those causing disease in humans. METHODS AND RESULTS:Faecal samples from 410 dairy cattle were collected from 36 farms in the Matamata-Piako district in New Zealand. Campylobacter jej...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03863.x
更新日期:2008-11-01 00:00:00
abstract:AIM:To investigate the aerobiology of the environment around mechanically ventilated broiler sheds with the aim of understanding dispersion in the surrounding environment. METHODS AND RESULTS:Aerosol samples were collected weekly on four different commercial broiler farms through the cycle of 55 days from 2005 to 2007...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04571.x
更新日期:2010-05-01 00:00:00
abstract:AIMS:To characterize atrazine-degrading potential of bacterial communities enriched from agrochemical factory soil by analysing diversity and organization of catabolic genes. METHODS AND RESULTS:The bacterial communities enriched from three different sites of varying atrazine contamination mineralized 65-80% of (14) C...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04700.x
更新日期:2010-07-01 00:00:00
abstract:AIMS:To develop a real-time PCR assay targeting the Escherichia coli flagellar antigen H21 for identification and surveillance of clinically important Shiga toxin-producing E. coli (STEC) serotypes classified in seropathotype C. METHODS AND RESULTS:The fliC allele of STEC O91:H21 strain B2F1 was amplified and sequence...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03607.x
更新日期:2008-03-01 00:00:00
abstract:AIM:To describe the relationship between the concentration of different indicator bacteria in red meat. METHODS AND RESULTS:Enumeration data for aerobic plate count (APC), Enterobacteriaceae, coliforms and Escherichia coli biotype I were analysed from an Australia-wide survey of beef carcasses, sheep carcasses, frozen...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03060.x
更新日期:2007-01-01 00:00:00
abstract:AIMS:The aim of this study was to investigate the influence of environmental and physiological factors on the susceptibility of Escherichia coli to the Curvularia haloperoxidase system. METHODS AND RESULTS:The Curvularia haloperoxidase system is a novel enzyme system that produces reactive oxygen species which have an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02491.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:The aim of this study was to develop a method based on immunomagnetic capture and polymerase chain reaction (IC-PCR assay) for detection of Cryptosporidium parvum and Giardia intestinalis in sewage sludge. METHODS AND RESULTS:The detection limit of the IC-PCR assay for both organisms was 625 oocysts and cysts ml(...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01468.x
更新日期:2001-12-01 00:00:00
abstract:AIMS:The aim was to isolate, identify and characterize yeasts present in rumen fluid and to select strains showing potential as probiotics. METHODS AND RESULTS:Rumen fluid was sampled from 4 herds of dairy and beef cattle and 77 yeast isolates were identified. Initial screening was based on the capacity to maintain vi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14350
更新日期:2019-09-01 00:00:00
abstract:AIMS:To isolate an iron-reducing bacterium and examine its ability of Fe(III) oxide reduction and dechlorination. METHODS AND RESULTS:A fermentative facultative anaerobe, strain L17 isolated from subterranean sediment, can reduce Fe(III) oxides and carbon tetrachloride (CT). It was identified as Klebsiella pneumoniae ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03985.x
更新日期:2009-01-01 00:00:00
abstract:AIMS:The phylum Chloroflexi is frequently found in high abundance in methanogenic reactors, but their role is still unclear as most of them remain uncultured and understudied. Hence, a detailed analysis was performed in samples from five up-flow anaerobic sludge blanket (UASB) full-scale reactors fed different industri...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14115
更新日期:2019-02-01 00:00:00
abstract:AIMS:Biofilms in water distribution systems represent a far more significant reservoir of micro-organisms than the water phase. Biofilms are (i) resistant to disinfectants, (ii) nuclei for microbial regrowth, (iii) a refuge for pathogens, (iv) accompanied by taste and odour problems, and (v) corrode surfaces. The effec...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01974.x
更新日期:2003-01-01 00:00:00
abstract::The changes in bacterial counts during the storage of a natural mineral water from a French spring were studied. Samples were taken from the spring and the bottling line. Viable cultivable (VC) bacteria were counted on R2A medium. Total counts, viable and dead bacteria were counted using the LIVE/DEAD Bac Light VIABIL...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00794.x
更新日期:1999-06-01 00:00:00