Abstract:
AIMS:Our main interest is to check if programmed cell death (PCD) can occur in prokaryotic algae and if the morphological and biochemical features of PCD are conserved. METHODS AND RESULTS:Using TUNEL labelling, fluorescence and light microscopy and DNA gel electrophoresis, we found that cell death with features similar to those in metazoan PCD could be induced in different Anabaena strains after exposure to univalent-cation salts at moderate concentration. These features included specific DNA fragmentation, cytoplasmic vacuolation, and the progressive disorganization, fragmentation and subsequent autolysis of the cell corpse. Further analyses of cell viability and proteinase activity revealed that increased protease activities, decreased DNA content, and loss of plasmalemma integrity were related to the PCD process. CONCLUSIONS:The results showed that like PCD in eukaryotes, PCD in Anabaena is an active process, and is an adaptation to adverse environments. The features of PCD shared between eukaryotes and Anabaena suggest that PCD mechanisms are conserved during evolution. SIGNIFICANCE AND IMPACT OF THE STUDY:The results will contribute greatly to our understanding of PCD origin and evolution, and are potentially useful in controlling the deluge of algae in some lakes.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Ning SB,Guo HL,Wang L,Song YCdoi
10.1046/j.1365-2672.2002.01651.xsubject
Has Abstractpub_date
2002-01-01 00:00:00pages
15-28issue
1eissn
1364-5072issn
1365-2672pii
1651journal_volume
93pub_type
杂志文章abstract:AIMS:To develop a method for studying the microbial spoilage of water-in-oil emulsions and to use this to investigate (i) the intrinsic stability of water-in-oil formulations and (ii) Pseudomonas aeruginosa SP1-induced spoilage of a proprietary emulsion. METHODS AND RESULTS:Aliquots of test emulsion were placed into w...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02239.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To evaluate the production and stability of laccases by Pleurotus ostreatus in liquid co-cultures with Trichoderma viride as a function of infection time and agitation rate. METHODS AND RESULTS:Pleurotus ostreatus cultures were infected with T. viride spores at 30 and 48 h. Maximal laccase volumetric activity was...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04493.x
更新日期:2010-03-01 00:00:00
abstract:AIMS:To search for culturable Burkholderia species associated with annual ryegrass in soils from natural pastures in Portugal, with plant growth-promoting effects. METHODS AND RESULTS:Annual ryegrass seedlings were used to trap Burkholderia from two different soils in laboratory conditions. A combined approach using g...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13025
更新日期:2016-03-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13604
更新日期:2018-01-01 00:00:00
abstract:AIMS:The goal was to study the effects of sub-minimum inhibitory concentrations (sub-MICs) of amoxicillin (AMX) on various physiological responses and virulence determinants in a commensal strain of Escherichia coli. MATERIALS AND RESULTS:The commensal strain was passaged under various sub-MICs of AMX and its effect o...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14987
更新日期:2021-01-02 00:00:00
abstract::The influence of growth phase on the evolution of cellular fatty acids (CFA) and survival of Sinorhizobium and Bradyrhizobium during desiccation and storage at different levels of relative humidity (R. H.) was studied. Lactobacillic, cis vaccenic and palmitic acids were the major fatty acids of S. meliloti RCR 2011, B...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00860.x
更新日期:1999-10-01 00:00:00
abstract:AIMS:Isolate and characterize a laccase-encoding gene (lac I) of Phlebia brevispora BAFC 633, as well as cloning and expressing cDNA of lac I in Pichia pastoris. And to obtain a purified and characterized recombinant laccase to analyse the biotechnological application potential. METHODS AND RESULTS:Lac I was cloned an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13720
更新日期:2018-06-01 00:00:00
abstract::The hygienic performances of the carcass dressing processes at 10 beef packing plants were assessed from small sets of microbiological data. For each process, a single sample was obtained from a randomly selected site on each of 25 randomly selected beef sides leaving the process. In addition, during a period of about...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00441.x
更新日期:1998-06-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04931.x
更新日期:2011-03-01 00:00:00
abstract:AIMS:Climate change is exerting an increasingly profound effect on grape composition, microbiology, chemistry and the sensory aspects of wine. Identification of autochthonous yeasts tolerant to stress could help to alleviate this effect. METHODS AND RESULTS:Tolerance to osmotic pressure, ethanol and pH of 94 Saccharom...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13139
更新日期:2016-07-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12858
更新日期:2015-09-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2018-05-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01771.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To understand the genetics involved in surface attachment and biofilm formation of Listeria monocytogenes. METHODS AND RESULTS:An in vitro screen of a Himar1 transposon library of L. monocytogenes strain 15G01 identified three transposants that produced significantly different biofilm levels when compared to the ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14790
更新日期:2020-07-20 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03648.x
更新日期:2008-04-01 00:00:00
abstract:AIMS:To compare survival of enterohaemorrhagic Escherichia coli (EHEC) strains of two clonal groups in a simulated gastric environment and to quantify the effect of storage in an acidic food, apple juice, on subsequent survival of EHEC in the simulated gastric environment. METHODS AND RESULTS:To characterize acid resi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03099.x
更新日期:2007-02-01 00:00:00
abstract:AIMS:To develop a DNA microarray for easy and fast detection of trichothecene- and moniliformin-producing Fusarium species. METHOD AND RESULTS:A DNA microarray was developed for detection and identification of 14 trichothecene- and moniliformin-producing species of the fungal genus Fusarium. The array could also diffe...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03165.x
更新日期:2007-04-01 00:00:00
abstract::The lethal toxin (LeTx) of Bacillus anthracis is the major virulence factor responsible for the death of infected animals and for cytolysis of cultured macrophages. Its catalytic component, LF, contains the characteristic zinc-binding motif of metalloproteases, it binds zinc and indirect evidence suggests that this hy...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00893.x
更新日期:1999-08-01 00:00:00
abstract:AIMS:Burkholderia sp. USM (JCM15050) isolated from oil-polluted wastewater is capable of utilizing palm oil products and glycerol to synthesize poly(3-hydroxybutyrate) [P(3HB)]. To confer the ability to produce polymer containing 3-hydroxyhexanoate (3HHx), plasmid (pBBREE32d13) harbouring the polyhydroxyalkanoate (PHA)...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.05189.x
更新日期:2012-01-01 00:00:00
abstract:AIM:Evaluation of the thermal and physical conditions for inactivation of adenovirus (AdV), porcine sapelovirus 1 (PSV1) and the economically important viruses porcine epidemic diarrhoea virus (PEDV) and porcine circovirus 2 (PCV2) in the production of spray-dried porcine plasma (SDPP). METHODS AND RESULTS:Citrate-tre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14235
更新日期:2019-06-01 00:00:00
abstract:AIMS:The current study aimed to assess the potential of a new high dose ultraviolet (UV) disinfection device to inactivate methicillin-resistant Staphylococcus aureus (MRSA), Clostridium difficile and a norovirus surrogate on handheld mobile devices, and to compare the efficacy of the UV-C device to hydrogen peroxide d...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14308
更新日期:2019-08-01 00:00:00
abstract:AIM:To investigate the antimicrobial effect of ionization on bacteria in household refrigerators. METHODS AND RESULTS:Ionizer prototypes were tested with respect to their technical requirements and their ability to reduce surface and airborne contamination in household refrigerators. Ion and ozone production of the te...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04359.x
更新日期:2009-12-01 00:00:00
abstract:AIMS:The gut microbiota capable of degrading plant biomass and antinutritional phytometabolites are of immense importance. This study reports isolation and characterization of tannase-producing rumen bacteria that could also degrade euptox A (9-oxo-10,11-dehydroageraphorone) present in Eupatorium adenophorum (Spreng). ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13563
更新日期:2017-11-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01646.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To assess the applicability of the 16S-23S rDNA internal spacer regions (ISR) as targets for PCR detection of Azospirillum ssp. and the phytostimulatory plant growth-promoting rhizobacteria seed inoculant Azospirillum lipoferum CRT1 in soil. METHODS AND RESULTS:Primer sets were designed after sequence analysis of...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04393.x
更新日期:2010-01-01 00:00:00
abstract:AIM:In this study, we investigated the effect of plagiochin E (PLE), a botanic-derived phenolic natural product, on reversal of fungal resistance to fluconazole (FLC) in vitro and the related mechanism. METHODS AND RESULTS:A synergistic action of PLE and FLC was observed in the FLC-resistant Candida albicans strains a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03617.x
更新日期:2008-03-01 00:00:00
abstract:AIMS:To develop an effective multiplex polymerase chain reaction (PCR) for the simultaneous detection of three important Vibrio species, Vibrio cholerae (Vc), V. parahaemolyticus (Vp) and V. vulnificus (Vv) using the groEL gene, a potential phylogenetic marker. METHODS AND RESULTS:Three species-specific primer sets we...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12056
更新日期:2013-02-01 00:00:00
abstract::Techniques for the separation/concentration of micro-organisms from background food matrices can be applied to increase the speed of analysis and ease of isolation and detection of target micro-organisms. One recent example of such a technique is the immunomagnetic separation (IMS) procedure that has been used for the...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00257.x
更新日期:1997-08-01 00:00:00
abstract:AIMS:Microbial induced calcium carbonate precipitation (MICP) is one of the bio-cementation methods for improving granular soils. This study evaluate the feasibility of obtaining a bacterial solution with high optical density and urease activity by an inexpensive corn steep liquor (CSL) medium in non-sterile conditions...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14866
更新日期:2020-10-07 00:00:00
abstract:AIMS:The objective of this study was the fundamental investigation of the antimicrobial efficiency of various hop extracts against selected foodborne pathogens in vitro, as well as their activity against Listeria monocytogenes in a model meat marinade and on marinated pork tenderloins. METHODS AND RESULTS:In a first s...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12717
更新日期:2015-03-01 00:00:00