Applicability of the 16S-23S rDNA internal spacer for PCR detection of the phytostimulatory PGPR inoculant Azospirillum lipoferum CRT1 in field soil.

Abstract:

AIMS:To assess the applicability of the 16S-23S rDNA internal spacer regions (ISR) as targets for PCR detection of Azospirillum ssp. and the phytostimulatory plant growth-promoting rhizobacteria seed inoculant Azospirillum lipoferum CRT1 in soil. METHODS AND RESULTS:Primer sets were designed after sequence analysis of the ISR of A. lipoferum CRT1 and Azospirillum brasilense Sp245. The primers fAZO/rAZO targeting the Azospirillum genus successfully yielded PCR amplicons (400-550 bp) from Azospirillum strains but also from certain non-Azospirillum strains in vitro, therefore they were not appropriate to monitor indigenous Azospirillum soil populations. The primers fCRT1/rCRT1 targeting A. lipoferum CRT1 generated a single 249-bp PCR product but could also amplify other strains from the same species. However, with DNA extracts from the rhizosphere of field-grown maize, both fAZO/rAZO and fCRT1/rCRT1 primer sets could be used to evidence strain CRT1 in inoculated plants by nested PCR, after a first ISR amplification with universal ribosomal primers. In soil, a 7-log dynamic range of detection (10(2)-10(8) CFU g(-1) soil) was obtained. CONCLUSIONS:The PCR primers targeting 16S-23S rDNA ISR sequences enabled detection of the inoculant A. lipoferum CRT1 in field soil. SIGNIFICANCE AND IMPACT OF THE STUDY:Convenient methods to monitor Azospirillum phytostimulators in the soil are lacking. The PCR protocols designed based on ISR sequences will be useful for detection of the crop inoculant A. lipoferum CRT1 under field conditions.

journal_name

J Appl Microbiol

authors

Baudoin E,Couillerot O,Spaepen S,Moënne-Loccoz Y,Nazaret S

doi

10.1111/j.1365-2672.2009.04393.x

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

25-38

issue

1

eissn

1364-5072

issn

1365-2672

pii

JAM4393

journal_volume

108

pub_type

杂志文章
  • Streptomyces effect on the bacterial microbiota associated to Crassostrea sikamea oyster.

    abstract:AIM:To determine the composition and diversity of the microbiota associated to Crassostrea sikamea treated during 30 days with Streptomyces strains N7 and RL8. METHODS AND RESULTS:DNA was extracted from oysters followed by 16S rRNA gene amplification and pyrosequencing. The highest and lowest species diversity richnes...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13382

    authors: García Bernal M,Trabal Fernández N,Saucedo Lastra PE,Medina Marrero R,Mazón-Suástegui JM

    更新日期:2017-03-01 00:00:00

  • Sensitive detection of Mycobacterium avium subsp. paratuberculosis in bovine semen by real-time PCR.

    abstract:AIMS:To develop a fast and sensitive protocol for detection of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine semen and to make a critical evaluation of the analytical sensitivity. METHODS AND RESULTS:Processed semen was spiked with known amounts of MAP. Semen from different bulls as well as semen of diff...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.02924.x

    authors: Herthnek D,Englund S,Willemsen PT,Bölske G

    更新日期:2006-05-01 00:00:00

  • Insights into the mechanisms of desiccation resistance of the Patagonian PAH-degrading strain Sphingobium sp. 22B.

    abstract:AIM:To analyse the physiological response of Sphingobium sp. 22B to water stress. METHODS AND RESULTS:The strain was grown under excess of carbon source and then subjected to low (60RH) and high (18RH) water stress conditions for 96 h. Quantification of trehalose, glycogen, polyhydroxybutyrate (PHB) and transmission e...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13742

    authors: Madueño L,Coppotelli BM,Festa S,Alvarez HM,Morelli IS

    更新日期:2018-06-01 00:00:00

  • Microelectronic-sensing assay to detect presence of Verotoxins in human faecal samples.

    abstract:AIMS: To develop a novel Vero cell assay that implements a real-time cell electronic sensing (RT-CES) system for the determination of the presence of verotoxin-producing Escherichia coli (VTEC). The assay overcomes the major drawbacks in conventional Vero cell assay, for example, labour-intensive and time-consuming. M...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05321.x

    authors: Xing JZ,Zhu L,Huang B,Chen J,Gabos S

    更新日期:2012-08-01 00:00:00

  • Copper-resistant halophilic bacterium isolated from the polluted Maruit Lake, Egypt.

    abstract:AIMS:To isolate and characterize copper-resistant halophilic bacteria from the polluted Maruit Lake, Egypt and identify the role of plasmids in toxic metal resistance. METHODS AND RESULTS:We isolated strain MA2, showing high copper resistance up to the 1.5 mmol l(-1) concentration; it was also resistant to other metal...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04574.x

    authors: Osman O,Tanguichi H,Ikeda K,Park P,Tanabe-Hosoi S,Nagata S

    更新日期:2010-04-01 00:00:00

  • A preliminary study of the effect of probiotic Streptococcus salivarius K12 on oral malodour parameters.

    abstract:AIMS:To determine whether dosing with bacteriocin-producing Streptococcus salivarius following an antimicrobial mouthwash effects a change in oral malodour parameters and in the composition of the oral microbiota of subjects with halitosis. MATERIALS AND RESULTS:Twenty-three subjects with halitosis undertook a 3-day r...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.02837.x

    authors: Burton JP,Chilcott CN,Moore CJ,Speiser G,Tagg JR

    更新日期:2006-04-01 00:00:00

  • Inactivation of Escherichia coli by citral.

    abstract:AIMS:The aim was to evaluate (i) the resistance of Escherichia coli BJ4 to citral in a buffer system as a function of citral concentration, treatment medium pH, storage time and initial inoculum size, (ii) the role of the sigma factor RpoS on citral resistance of E. coli, (iii) the role of the cell envelope damage in t...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04597.x

    authors: Somolinos M,García D,Condón S,Mackey B,Pagán R

    更新日期:2010-06-01 00:00:00

  • Prevalence, phenotypic traits and molecular characterization of emetic toxin-producing Bacillus cereus strains isolated from human stools in Korea.

    abstract:AIMS:To investigate the prevalence and genotypic/phenotypic characters of emetic toxin-producing Bacillus cereus strains isolated from sporadic food poisoning cases in Korea. METHODS AND RESULTS:The prevalence of emetic B. cereus was determined in 56 899 stool samples from sporadic food poisoning cases in Korea betwee...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05277.x

    authors: Chon JW,Kim JH,Lee SJ,Hyeon JY,Song KY,Park C,Seo KH

    更新日期:2012-05-01 00:00:00

  • Apple bagasse as a substrate for the propagation of Patagonian wine yeast biomass.

    abstract:AIMS:A culture medium based on apple bagasse was designed and tested as a substrate for biomass production of conventional and unconventional native wine yeasts. METHODS AND RESULTS:The physicochemical characterization of the apple bagasse was carried out and its potential utility as a constituent of a complete cultur...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14216

    authors: Bravo SME,Morales M,Del Mónaco SM,Caballero AC

    更新日期:2019-05-01 00:00:00

  • Nutritive value of treated Quercus infectoria and Quercus libani leaves with the tannin-degrading bacterium Klebsiella pneumoniae for ruminant feeding in vitro.

    abstract:AIMS:This study was conducted to evaluate the chemical composition and in vitro gas production (GP) and fermentation parameters of Quercus infectoria and Quercus libani leaves following treatment with the Klebsiella pneumoniae, a tannin-degrading bacterium. METHODS AND RESULTS:This isolate was isolated on medium conta...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14408

    authors: Motamedi H,Azizi A,Ahmadi M

    更新日期:2019-11-01 00:00:00

  • Bacillus subtilis AB1 controls Aeromonas infection in rainbow trout (Oncorhynchus mykiss, Walbaum).

    abstract:AIM:To develop a probiotic with effectiveness against Aeromonas sp., which was pathogenic to rainbow trout. METHODS AND RESULTS:When Bacillus subtilis AB1, which was obtained from fish intestine, was administered for 14 days to rainbow trout in feed at a concentration of 10(7) cells per gram either as viable, formaliz...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03402.x

    authors: Newaj-Fyzul A,Adesiyun AA,Mutani A,Ramsubhag A,Brunt J,Austin B

    更新日期:2007-11-01 00:00:00

  • Three-percent sucrose acts as a thermostabilizer for cell-adapted foot-and-mouth disease virus without any negative effect on viral growth.

    abstract:AIMS:As cell-adapted foot-and-mouth disease virus (FMDV) with H56R mutation in VP3 has reduced thermostability, this study aimed to investigate the effect of thermostabilizers on cell-adapted FMDV for vaccine production. METHODS AND RESULTS:We examined the effect of 3% sucrose, 10% (or 25%) glycerol or 10% FBS on cell...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14565

    authors: Hwang JH,Moon Y,Lee G,Kim MY,Lee KN,Park JH,Lee M,Kim B,Kim SM

    更新日期:2020-05-01 00:00:00

  • Cytotoxicity and apoptosis induction of some selected marine bacteria metabolites.

    abstract:AIMS:To study the potential apoptosis effects of cytotoxic marine bacterial metabolites on human HeLa cell line. METHODS AND RESULTS:After HeLa cells were routinely cultured, tetrazolium-based colorimetric assay for cytotoxicity was performed to screen the marine bacteria extracts showing 12 strains active. To find th...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02741.x

    authors: Lin J,Yan XJ,Zheng L,Ma HH,Chen HM

    更新日期:2005-01-01 00:00:00

  • Isolation and characterization of antifungal compound from Lactobacillus plantarum KCC-10 from forage silage with potential beneficial properties.

    abstract:AIMS:The purpose of this study was to isolate, identify and characterize an antifungal compound from Lactobacillus plantarum KCC-10 from forage silage with potential beneficial properties. METHODS AND RESULTS:The 16S rRNA gene-based phylogenetic affiliation was determined using bioinformatic tools and identified as La...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12319

    authors: Valan Arasu M,Jung MW,Ilavenil S,Jane M,Kim DH,Lee KD,Park HS,Hur TY,Choi GJ,Lim YC,Al-Dhabi NA,Choi KC

    更新日期:2013-11-01 00:00:00

  • Antimicrobial activity in culturable gut microbial communities of springtails.

    abstract:AIMS:The rise of antibiotic resistance pushes the pharmaceutical industry to continually search for substances with new structures and novel mechanisms of action. Many environmental niches are still to be explored as sources of antimicrobials. In this paper, we assess the antimicrobial potential of gut microbes of spri...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13899

    authors: Agamennone V,Roelofs D,van Straalen NM,Janssens TKS

    更新日期:2018-09-01 00:00:00

  • Nutritional requirements and simplified cultivation medium to study growth and energetics of a sourdough lactic acid bacterium Lactobacillus fermentum Ogi E1 during heterolactic fermentation of starch.

    abstract:AIMS:Nutritional requirements of Lactobacillus fermentum Ogi E1 were studied in order to define a simplified fermentation medium. METHODS AND RESULTS:When grown with MRS-medium in 2l bioreactors, a biphasic pattern of growth and metabolite production was observed. Study of nutritional requirements resulted in a simpli...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01272.x

    authors: Calderon M,Loiseau G,Guyot JP

    更新日期:2001-04-01 00:00:00

  • Interleukin-8 gene expression and apoptosis induced by Salmonella Typhimurium in the presence of Bacillus probiotics in the epithelial cell.

    abstract:AIMS:This study aimed to evaluate the effects of three Bacillus probiotics on Salmonella Typhimurium, and interleukin-8 (IL-8) gene expression in the co-culture of the Bacillus and the pathogen in vitro. METHODS AND RESULTS:Bacillus subtilis, Bacillus indicus and Bacillus coagulans were initially turned to spore and h...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14898

    authors: Kawarizadeh A,Pourmontaseri M,Farzaneh M,Hosseinzadeh S,Ghaemi M,Tabatabaei M,Pourmontaseri Z,Pirnia MM

    更新日期:2020-10-15 00:00:00

  • Comparison of adhesion of wound isolates of Staphylococcus aureus to immobilized proteins.

    abstract:AIMS:To determine the ability of 149 clinical isolates of Staphylococcus aureus from burns, other wounds and environmental isolates to adhere to immobilized proteins. METHODS AND RESULTS:The ability to bind to immobilized fibrinogen, fibronectin, laminin, collagen, IgG and lysozyme was studied using a microtitre plate...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01858.x

    authors: Elgalai I,Foster HA

    更新日期:2003-01-01 00:00:00

  • Taxonomic and functional diversity of atrazine-degrading bacterial communities enriched from agrochemical factory soil.

    abstract:AIMS:To characterize atrazine-degrading potential of bacterial communities enriched from agrochemical factory soil by analysing diversity and organization of catabolic genes. METHODS AND RESULTS:The bacterial communities enriched from three different sites of varying atrazine contamination mineralized 65-80% of (14) C...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04700.x

    authors: Udiković-Kolić N,Hršak D,Devers M,Klepac-Ceraj V,Petrić I,Martin-Laurent F

    更新日期:2010-07-01 00:00:00

  • Production and characterization of a new bioemulsifier from Pseudomonas putida ML2.

    abstract:AIMS:To characterize the bioemulsifier produced by a nonfluorescent strain of Pseudomonas putida isolated from a polluted sediment and to determine the influence of pH, temperature, media composition, and carbon and nitrogen source on growth and emulsifying activity. METHODS AND RESULTS:Different indexes were employed...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02480.x

    authors: Bonilla M,Olivaro C,Corona M,Vazquez A,Soubes M

    更新日期:2005-01-01 00:00:00

  • Optimization of soybean processing into kinema, a Bacillus-fermented alkaline food, with respect to a minimum level of antinutrients.

    abstract:AIMS:Optimization of traditional processing of soybeans using response surface methodology (RSM) to achieve a minimum level of antinutritional factors (ANFs) in kinema. METHODS AND RESULTS:Central composite rotatable designs were used to optimize the processing stages of kinema preparation. In each stage, the linear o...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12826

    authors: Sharma A,Kumari S,Wongputtisin P,Nout MJ,Sarkar PK

    更新日期:2015-07-01 00:00:00

  • In vitro effects of sub-inhibitory concentrations of amoxicillin on physiological responses and virulence determinants in a commensal strain of Escherichia coli.

    abstract:AIMS:The goal was to study the effects of sub-minimum inhibitory concentrations (sub-MICs) of amoxicillin (AMX) on various physiological responses and virulence determinants in a commensal strain of Escherichia coli. MATERIALS AND RESULTS:The commensal strain was passaged under various sub-MICs of AMX and its effect o...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14987

    authors: Chadha J

    更新日期:2021-01-02 00:00:00

  • Analysis of the germination of individual Clostridium perfringens spores and its heterogeneity.

    abstract:AIMS:To analyse the germination and its heterogeneity of individual spores of Clostridium perfringens. METHODS AND RESULTS:Germination of individual wild-type Cl. perfringens spores was followed by monitoring Ca-dipicolinic acid (CaDPA) release and by differential interference contrast (DIC) microscopy. Following the ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05135.x

    authors: Wang G,Zhang P,Paredes-Sabja D,Green C,Setlow P,Sarker MR,Li YQ

    更新日期:2011-11-01 00:00:00

  • Physiological characterization of Mycobacterium sp. strain 1B isolated from a bacterial culture able to degrade high-molecular-weight polycyclic aromatic hydrocarbons.

    abstract:AIM:The aim of this study was to further characterize a bacterial culture (VUN 10,010) capable of benzo[a]pyrene cometabolism. METHODS AND RESULTS:The bacterial culture, previously characterized as a pure culture of Stenotrophomonas maltophilia (VUN 10,010), was found to also contain another bacterial species (Mycobac...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02087.x

    authors: Dandie CE,Thomas SM,Bentham RH,McClure NC

    更新日期:2004-01-01 00:00:00

  • In vitro potential antioxidant activity of indigenous yeasts isolated from virgin olive oil.

    abstract:AIM:The aim of the present study was to investigate the in vitro antioxidant activity of yeast strains isolated from virgin olive oil. METHODS AND RESULTS:The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free-radical scavenging activity of single cultures of 24 yeast strains belonging to eight species isolated from virgin ol...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14520

    authors: Ciafardini G,Zullo BA

    更新日期:2020-03-01 00:00:00

  • Differences in major bacterial populations in the intestines of mature broilers after feeding virginiamycin or bacitracin methylene disalicylate.

    abstract:AIMS:The purpose of this study was to compare the effects of feeding virginiamycin or bacitracin methylene disalicylate (BMD), two in-feed antibiotics typically used by commercial poultry producers in the United States, on the chicken gastrointestinal microbiota. METHODS AND RESULTS:454 pyrosequencing of the V6-V8 reg...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12960

    authors: Neumann AP,Suen G

    更新日期:2015-12-01 00:00:00

  • Effectiveness of egg yolk immunoglobulin against the intracellular salmonid pathogen Piscirickettsia salmonis.

    abstract:AIMS:To produce and characterize egg yolk immunoglobulin (IgY) against the fish intracellular pathogen Piscirickettsia salmonis as well as to evaluate the antibacterial activity of IgY in vitro and the availability in the serum of fish immunized orally. METHODS AND RESULTS:Specific IgY was produced by immunizing hens ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12857

    authors: Oliver C,Valenzuela K,Silva H,Haro RE,Cortés M,Sandoval R,Pontigo JP,Álvarez C,Figueroa JE,Avendaño-Herrera R,Troncoso JM,Yáñez AJ

    更新日期:2015-08-01 00:00:00

  • Global gene expression of Trichophyton rubrum in response to PH11B, a novel fatty acid synthase inhibitor.

    abstract:AIMS:To determine the transcriptional responses of Trichophyton rubrum to the artificial substance, PH11B. METHODS AND RESULTS:The broth microdilution assay for antifungal susceptibility testing of dermatophytes was used to measure the minimum inhibitory concentration (MIC) of PH11B. cDNA microarray technology and rea...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03521.x

    authors: Yu L,Zhang W,Liu T,Wang X,Peng J,Li S,Jin Q

    更新日期:2007-12-01 00:00:00

  • Identification and prevalence of an enterotoxin-related gene, se-int, in Staphylococcus intermedius isolates from dogs and pigeons.

    abstract:AIMS:To determine the prevalence of enterotoxin-producing Staphylococcus intermedius in dogs and pigeons. METHODS AND RESULTS:A total of 106 S. intermedius isolates from 44 dogs and 62 pigeons were tested for the production of enterotoxins A, B, C and D by reverse passive latex agglutination (RPLA) and for sec-canine ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02264.x

    authors: Futagawa-Saito K,Suzuki M,Ohsawa M,Ohshima S,Sakurai N,Ba-Thein W,Fukuyasu T

    更新日期:2004-01-01 00:00:00

  • Flow cytometry: a versatile technology for specific quantification and viability assessment of micro-organisms in multistrain probiotic products.

    abstract:AIMS:Classical microbiology techniques are the gold standard for probiotic enumeration. However, these techniques are limited by parameters of time, specificity and incapacity to detect viable but nonculturable (VBNC) micro-organisms and nonviable cells. The aim of the study was to evaluate flow cytometry as a novel me...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13666

    authors: Chiron C,Tompkins TA,Burguière P

    更新日期:2018-02-01 00:00:00