Abstract:
:Techniques for the separation/concentration of micro-organisms from background food matrices can be applied to increase the speed of analysis and ease of isolation and detection of target micro-organisms. One recent example of such a technique is the immunomagnetic separation (IMS) procedure that has been used for the separation of specific micro-organisms from foods. This paper describes the use of a novel biosorbent consisting of a Salmonella-specific bacteriophage (phage) immobilized to a solid phase that was used for the separation and concentration of Salmonella from food materials. This work has shown that a Salmonella-specific phage-based biosorbent could remove Salmonella from culture fluid and separate Salmonella from suspensions of other Enterobacteriaceae. The ease of production of phage, high affinity of phage-cell interaction and the ability of phage to infect host cells in heterogeneous environments indicates the potential of such a biosorbent as the basis for a reliable separation system in food microbiological analysis.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Bennett AR,Davids FG,Vlahodimou S,Banks JG,Betts RPdoi
10.1046/j.1365-2672.1997.00257.xsubject
Has Abstractpub_date
1997-08-01 00:00:00pages
259-65issue
2eissn
1364-5072issn
1365-2672journal_volume
83pub_type
杂志文章abstract:AIMS:The objective of this study was to investigate the effect of temperature (5-25°C) on epiphytic lactic acid bacteria (LAB) populations during 60 days of fermentation of whole-plant corn silage. METHODS AND RESULTS:Vacuum bag mini-silos of chopped whole-plant corn were incubated at five different temperatures (5, 1...
journal_title:Journal of applied microbiology
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更新日期:2016-09-01 00:00:00
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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更新日期:2006-07-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2013-04-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04008.x
更新日期:2009-02-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00631.x
更新日期:1999-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14226
更新日期:2019-06-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1111/jam.14068
更新日期:2018-08-14 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00312.x
更新日期:1998-01-01 00:00:00
abstract:AIMS:The present work aimed to obtain bioinsecticide over-producing mutants through classical mutagenesis of vegetative cells of Bacillus thuringiensis (Bt) by using u.v. and nitrous acid, and to evidence the involvement of cell metabolism in delta-endotoxin synthesis. METHODS AND RESULTS:Vegetative cells of Bt were t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12651
更新日期:2014-12-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02371.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To avoid interference by water-iodine disinfection chemistry and measure directly the effect of iodine, captured from a triiodide complex bound to a filter medium, on viability of penetrating viral particles. METHODS AND RESULTS:Aerosols of MS2 coli phage were passed through control P100 or iodinated High-Efficie...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12788
更新日期:2015-06-01 00:00:00
abstract:AIMS:To evaluate four DNA extraction methods to elucidate the most effective method for bacterial DNA recovery from human milk (HM). METHODS AND RESULTS:Human milk DNA was extracted using the following methods: (i) Qiagen MagAttract Microbial DNA Isolation Kit (kit QM), (ii) Norgen Milk Bacterial DNA Isolation Kit (ki...
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pub_type: 杂志文章
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更新日期:2021-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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更新日期:2009-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00481.x
更新日期:1998-07-01 00:00:00
abstract:AIMS:Utilization of silage in livestock farming is expected to increase in developing countries in the tropical and subtropical parts of the world. The aim of this study was to investigate the influence of nitrogen fertilization on the chemical composition of herbage, ensiling process and silage quality, and to contrib...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04591.x
更新日期:2010-06-01 00:00:00
abstract:AIMS:The medicinal fungi Inonotus xeranticus and Phellinus linteus in the family Hymenochaetaceae have been used as traditional medicines for the treatment of various diseases. However, the compound responsible for the antioxidant activity is still unknown. Therefore, this study was conducted to characterize the antiox...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03737.x
更新日期:2008-06-01 00:00:00
abstract:AIMS:The trans-10,cis-12 conjugated linoleic acid (CLA) is known for its antilipogenic effect but the mechanism is not fully clear. In this study, the potential of yeast (Saccharomyces cerevisiae) metabolism to offer evidence for the mechanism was investigated. METHODS AND RESULTS:The inhibitory effect of CLA on lipid...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13443
更新日期:2017-07-01 00:00:00
abstract::An extracellular alpha-amylase gene from the hyperthermophilic archaeon Pyrococcus woesei has been cloned and sequenced. The 1.4-kb protein-coding sequence is identical to that of the corresponding alpha-amylase gene of the closely related species P. furiosus. By using a shuttle cloning vector for halophilic bacteria,...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.00988.x
更新日期:2000-03-01 00:00:00
abstract:AIMS:To evaluate an integrated cell culture (ICC)-multiplex-nested PCR using the buffalo green monkey kidney (BGMK) cells for the simultaneous detection of both enteroviruses and adenoviruses in surface water and tap water samples and optimize the procedure for more sensitive detection of virus showing no apparent cyto...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02496.x
更新日期:2005-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13510
更新日期:2017-11-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02532.x
更新日期:2005-01-01 00:00:00
abstract::A bacterium (strain TJ330) capable of using carbon disulphide (CS2) as its sole energy source in an acidic environment was isolated from a peat biofilter used in experiments to remove CS2 and hydrogen sulphide (H2S) from air. Its physiology and taxonomy are described here. The strain oxidized CS2, H2S and elemental su...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01150.x
更新日期:2000-10-01 00:00:00
abstract:AIMS:To evaluate the performance of three Salmonella plating media (Rambach, Xylose Lysine Deoxycholate agar and modified Brilliant Green Agar plus Novobiocin) as part of the ISO 6579: 2002 (Annex D) on poultry environmental samples. METHODS AND RESULTS:The samples analysed were those for the European Union Salmonella...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04386.x
更新日期:2009-12-01 00:00:00