Abstract:
AIMS:Utilization of silage in livestock farming is expected to increase in developing countries in the tropical and subtropical parts of the world. The aim of this study was to investigate the influence of nitrogen fertilization on the chemical composition of herbage, ensiling process and silage quality, and to contribute to the improvement of tropical-grass silage preparation. METHODS AND RESULTS:Guinea grass grown under two different nitrogen-fertilizer application conditions [1.5 kg N a(-1) (high-N) and 0.5 kg N a(-1) (low-N)] was packed in plastic bags, and its ensiling process was investigated by chemical and microbial-community analyses. Relatively well-preserved silage was obtained from high-N herbage, which accumulated a high nitrate concentration. Denaturing gradient gel electrophoresis analysis revealed that Lactobacillus plantarum dominated throughout the ensiling of high-N herbage and in the early phase of that of low-N herbage. In low-N silages prepared from ammonium sulfate- and urea-fertilized herbage, Lact. plantarum was replaced by clostridia after 40 and 15 days of ensiling, respectively. CONCLUSIONS:Nitrate content of herbage is an important factor that influences silage quality, and careful fertilization management can facilitate stable and successful fermentation of tropical-grass silage without any pretreatment. SIGNIFICANCE AND IMPACT OF THE STUDY:The positive effect of nitrate on the ensiling process of tropical-grass was proved by microbial-community analysis.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Namihira T,Shinzato N,Akamine H,Maekawa H,Matsui Tdoi
10.1111/j.1365-2672.2009.04591.xsubject
Has Abstractpub_date
2010-06-01 00:00:00pages
1954-65issue
6eissn
1364-5072issn
1365-2672pii
JAM4591journal_volume
108pub_type
杂志文章abstract:AIMS:To analyse the morphological and ultrastructural effects of lipopeptides of cell-free liquid cultures from the antagonistic Bacillus subtilis strains, UMAF6614 and UMAF6639, on the cucurbit powdery mildew fungus, Podosphaera fusca, conidial germination. METHODS AND RESULTS:Butanolic extracts from cell-free supern...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03323.x
更新日期:2007-10-01 00:00:00
abstract:AIMS:Our main interest is to check if programmed cell death (PCD) can occur in prokaryotic algae and if the morphological and biochemical features of PCD are conserved. METHODS AND RESULTS:Using TUNEL labelling, fluorescence and light microscopy and DNA gel electrophoresis, we found that cell death with features simil...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01651.x
更新日期:2002-01-01 00:00:00
abstract:AIM:To evaluate Thermus scotoductus SA-01's ability to reduce Cr(VI) aerobically. METHODS AND RESULTS:T. scotoductus SA-01 is able to reduce Cr(VI) aerobically when grown in a complex organic medium containing Cr(VI) concentrations up to 0.5 mmol l(-1). Suspension of T. scotoductus SA-01 cells also reduced Cr(VI) aero...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03429.x
更新日期:2007-11-01 00:00:00
abstract:AIMS:To investigate the effect of media composition and agroindustrial residues on bovicin HC5 production by Streptococcus bovis HC5. METHODS AND RESULTS:Batch cultures of S. bovis HC5 were grown in basal medium containing different carbon and nitrogen sources. The activity of cell-free and cell-associated bovicin HC5...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04212.x
更新日期:2009-07-01 00:00:00
abstract:AIMS:To investigate the prevalence, seasonality and genotype distribution of human noroviruses (NoVs) in wastewater in Japan. METHODS AND RESULTS:Influent and effluent water samples were collected monthly for a year from a wastewater treatment plant and examined for the presence of genogroups I and II (GI and GII) NoV...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05231.x
更新日期:2012-03-01 00:00:00
abstract::The ability of artificial neural networks (ANN), fuzzy systems (FS) and multiple linear regression (MLR) to fit the biological activity surface describing the inhibition of Listeria monocytogenes by benzoic and cinnamic acid derivatives was compared. MLR and ANN were also compared for their ability to select the prope...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract:AIMS:To optimize the nutritional and environmental conditions for growth of and poly-beta-hydroxybutyrate (PHB) accumulation in Bacillus mycoides RLJ B-017. METHODS AND RESULTS:An isolate, identified as B. mycoides, was grown on different sources of carbon and nitrogen. Among these, sucrose, beef extract and di-ammoni...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01590.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To investigate the synthesis of selected bioactive compounds in various strains belonging to the Arthrospira genus. METHODS AND RESULTS:The synthesis of vitamin C, polyphenols, vitamin B12, fatty acids and selected volatile compounds (acetone and acetaldehyde) was investigated in six strains belonging to the Arth...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12897
更新日期:2015-09-01 00:00:00
abstract:AIMS:Probiotics have the ability to enhance the immune system, produce anti-inflammatory action and promote wound healing process. The first aim of the study was to isolate pathogenic micro-organisms from sites of chronic ulcerative lesion. The second aim was to evaluate probiotic efficacy of SYNBIO® (1:1 combination o...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14482
更新日期:2020-02-01 00:00:00
abstract:AIMS:To characterize Lactobacillus strains with EPS-producing ability compared with non-EPS-producing lactobacilli of the same species for technological performance including simulated gastrointestinal tract (GIT) conditions. METHODS AND RESULTS:Characterization of EPS-producing Lactobacillus mucosae DPC 6426 in detai...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12542
更新日期:2014-08-01 00:00:00
abstract:AIMS:This study describes a novel in vitro assay that simultaneously determines antifungal efficiency and host cell toxicity using suspensions of human leukaemic cells (HL-60) infected with Candida albicans. METHODS AND RESULTS:The effect of Candida infection on host cell viability was evaluated by the microscopy of t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12817
更新日期:2015-07-01 00:00:00
abstract:AIMS:This study examined the tolerance of various species of the genus Bifidobacterium to heat and oxygen and evaluated the survival of selected strains following spray drying and during storage. METHODS AND RESULTS:Nine Bifidobacterium species were considered to be relatively tolerant to both heat and oxygen and most...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02648.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To enhance the recovery of f(2) bacteriophage and poliovirus by an established method based on the adsorption to and elution from positively-charged Al(OH)(3)-treated silica gel. METHODS AND RESULTS:Polyaluminum Chloride (PAC) was added to water samples to neutralize the negatively charged materials, which can re...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04041.x
更新日期:2009-02-01 00:00:00
abstract:AIM:This study was performed to isolate and characterize novel antifungal lipopeptide from Bacillus cereus. METHODS AND RESULTS:Elucidation of its chemical structure was carried out by electrospray ionization mass spectra (ESI-MS) and Fourier transform infrared spectroscopy (FT-IR). The compound is a cyclic heptapepti...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12324
更新日期:2013-12-01 00:00:00
abstract:AIMS:Analysis of the physiology and metabolism of Escherichia coli arcA and creC mutants expressing a bifunctional alcohol-acetaldehyde dehydrogenase from Leuconostoc mesenteroides growing on glycerol under oxygen-restricted conditions. The effect of an ldhA mutation and different growth medium modifications was also a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04668.x
更新日期:2010-08-01 00:00:00
abstract:AIMS:To investigate the genetic basis of Cr(VI) resistance and its reduction to Cr(III) in indigenous bacteria isolated from tannery effluent. METHODS AND RESULTS:Four bacteria resistant to high Cr(VI) levels were isolated and identified as Bacillus spp. Their Cr(VI) reduction ability was tested. To assess the genetic...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04326.x
更新日期:2009-11-01 00:00:00
abstract:AIMS:To investigate the factors affecting expression and solubilization of Escherichia coli maltodextrin glucosidase in E. coli. METHODS AND RESULTS:Expression level and solubilization of the recombinant E. coli maltodextrin glucosidase was studied in E. coli at different temperatures, in presence of overexpressed Gro...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03519.x
更新日期:2008-01-01 00:00:00
abstract::The survival and environmental spread of Salmonella bacteria from domestic toilets was examined in homes, where a family member had recently suffered an attack of salmonellosis. In four out of six households tested, Salmonella bacteria persisted in the biofilm material found under the recess of the toilet bowl rim whi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01091.x
更新日期:2000-07-01 00:00:00
abstract:AIMS:The objective of this study was to determine antimicrobial activities of essential oils (EOs) against bovine respiratory disease (BRD) pathogens and nasopharyngeal commensal bacteria, as well as cytotoxicity in bovine turbinate (BT) cells in vitro. METHODS AND RESULTS:The chemical composition of 16 EOs was determ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14238
更新日期:2019-06-01 00:00:00
abstract:AIMS:The cell-surface display of chitinase Chi9602ΔSP was constructed on Bacillus thuringiensis BMB171 by using two repeat N-terminal regions of autolysin (Mbgn)2 as the anchoring motif. This study aimed to prepare a chitinolytic whole-cell B. thuringiensis biocatalyst with improved catalytic and antifungal activities....
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13333
更新日期:2017-01-01 00:00:00
abstract:AIMS:To evaluate the capacity of Oenococcus oeni strains to release aroma compounds from glycosylated precursors by measuring glycosidase activities with both synthetic and natural substrates. METHODS AND RESULTS:Five glycosidase activities were investigated in 47 O. oeni strains using synthetic substrates. This scree...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04878.x
更新日期:2011-01-01 00:00:00
abstract:AIMS:Lactic acid bacteria (LAB) were isolated and sequenced from the faeces of healthy dogs. Five of these strains were selected and further characterized to clarify the potential of these strains as probiotics for canine. METHODS AND RESULTS:LAB were found in 67% (14/21) of the canine faeces samples when plated on La...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02884.x
更新日期:2006-07-01 00:00:00
abstract:AIMS:To add a spore germination step in order to reduce decontamination temperature and time requirements compared to the current hot, humid air decontamination parameters, which are 75-80°C, ≥72 h, 70-90% RH, down to ≤60°C and ≤24 h total decontamination time. METHODS AND RESULTS:Bacillus anthracis spore germination ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14474
更新日期:2020-01-01 00:00:00
abstract:AIMS:To identify and examine the diversity of predominant lactic acid bacteria (LAB) in koko and koko sour water (KSW) from different Ghanaian production sites with regard to pattern of fermentation (API 50 CHL), genotype, antimicrobial activity, and resistance to low pH and bile salts. METHODS AND RESULTS:In total 21...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2004.02162.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:Implant-associated infections arise from the formation of bacterial biofilms, which are difficult to be treated with conventional antibiotics. Therefore, there is a need for new implant functionalizations, which inhibit biofilm formation. The aim of the present study was to characterize the effect of synthetic pep...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14701
更新日期:2020-11-01 00:00:00
abstract:AIMS:We aimed to systematically understand the composting processes by a comparison of microbial communities during four full-scale composting processes. METHODS AND RESULTS:Microbial communities during the four different full-scale composting processes were analysed by denaturing gradient gel electrophoresis combined...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01949.x
更新日期:2003-01-01 00:00:00
abstract::The effect of electroporation (very short duration pulses of high voltage electricity) on the viability of Giardia cysts and Cryptosporidium oocysts, and on the viability of these organisms in the presence of free chlorine, combined chlorine, hydrogen peroxide and potassium permanganate, was examined. While electropor...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00725.x
更新日期:1999-06-01 00:00:00
abstract:AIM:To investigate the aerobiology of the environment around mechanically ventilated broiler sheds with the aim of understanding dispersion in the surrounding environment. METHODS AND RESULTS:Aerosol samples were collected weekly on four different commercial broiler farms through the cycle of 55 days from 2005 to 2007...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04571.x
更新日期:2010-05-01 00:00:00
abstract:AIMS:The aims of this study were to identify the structure of antibacterial lipopeptide (LP) produced by Bacillus amyloliquefaciens strain FJAT-2349, to analyse the effects of the culture medium and temperature on LP production and to assess the biocontrol efficiency of the LPs against tomato bacterial wilt. METHODS A...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14213
更新日期:2019-05-01 00:00:00
abstract:AIMS:To investigate low molecular weight compounds produced in vitro by Lysobacter capsici AZ78 and their toxic activity against sporangia of plant pathogenic oomycetes. METHODS AND RESULTS:Assays carried out in vitro showed that L. capsici AZ78 drastically inhibits the growth of plant pathogenic oomycetes. Accordingl...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12611
更新日期:2014-10-01 00:00:00