Influence of nitrogen fertilization on tropical-grass silage assessed by ensiling process monitoring using chemical and microbial community analyses.

Abstract:

AIMS:Utilization of silage in livestock farming is expected to increase in developing countries in the tropical and subtropical parts of the world. The aim of this study was to investigate the influence of nitrogen fertilization on the chemical composition of herbage, ensiling process and silage quality, and to contribute to the improvement of tropical-grass silage preparation. METHODS AND RESULTS:Guinea grass grown under two different nitrogen-fertilizer application conditions [1.5 kg N a(-1) (high-N) and 0.5 kg N a(-1) (low-N)] was packed in plastic bags, and its ensiling process was investigated by chemical and microbial-community analyses. Relatively well-preserved silage was obtained from high-N herbage, which accumulated a high nitrate concentration. Denaturing gradient gel electrophoresis analysis revealed that Lactobacillus plantarum dominated throughout the ensiling of high-N herbage and in the early phase of that of low-N herbage. In low-N silages prepared from ammonium sulfate- and urea-fertilized herbage, Lact. plantarum was replaced by clostridia after 40 and 15 days of ensiling, respectively. CONCLUSIONS:Nitrate content of herbage is an important factor that influences silage quality, and careful fertilization management can facilitate stable and successful fermentation of tropical-grass silage without any pretreatment. SIGNIFICANCE AND IMPACT OF THE STUDY:The positive effect of nitrate on the ensiling process of tropical-grass was proved by microbial-community analysis.

journal_name

J Appl Microbiol

authors

Namihira T,Shinzato N,Akamine H,Maekawa H,Matsui T

doi

10.1111/j.1365-2672.2009.04591.x

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

1954-65

issue

6

eissn

1364-5072

issn

1365-2672

pii

JAM4591

journal_volume

108

pub_type

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