Abstract:
AIMS:To add a spore germination step in order to reduce decontamination temperature and time requirements compared to the current hot, humid air decontamination parameters, which are 75-80°C, ≥72 h, 70-90% RH, down to ≤60°C and ≤24 h total decontamination time. METHODS AND RESULTS:Bacillus anthracis spore germination with l-alanine+inosine+calcium dipicolinate (CaDPA) was quantified at 0-40°C, several time points and spore concentrations of 5-9 log10 per ml. Germination efficiency at 0-40°C was >99% at <8 log10 spores per ml. The temperature optimum was 20°C. Germination efficiency was significantly higher but slower at 0°C compared to ≥30°C at ≥8 log10 spores per ml. A single germinant application followed by 60°C, 1-h treatment consistently inactivated >2 log10 (>99%) of spores. However, a repeat application of germinant was needed to achieve the objective of ≥6 log10 spore inactivation out of a 7 log10 challenge (≥99·9999%) for ≤24 h total decontamination time for nylon and aircraft performance coating. CONCLUSIONS:l-alanine+inosine+CaDPA stimulated germination across wide temperature and spore concentration ranges. SIGNIFICANCE AND IMPACT OF THE STUDY:Germination expands the scope of spore decontamination to include materials from any industry sector that can be sprayed with an aqueous germinant solution.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Buhr TL,Minter ZA,Kennihan NL,Young AA,Borgers-Klonkowski EL,Osborn EB,Bohmke MD,Hamilton SM,Kimani MB,Miller CT,Mackie RS,Innocenti JM,Bensman MD,Lilly SDdoi
10.1111/jam.14474subject
Has Abstractpub_date
2020-01-01 00:00:00pages
124-137issue
1eissn
1364-5072issn
1365-2672journal_volume
128pub_type
杂志文章abstract:AIMS:The purpose of this study was to compare the effects of feeding virginiamycin or bacitracin methylene disalicylate (BMD), two in-feed antibiotics typically used by commercial poultry producers in the United States, on the chicken gastrointestinal microbiota. METHODS AND RESULTS:454 pyrosequencing of the V6-V8 reg...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12960
更新日期:2015-12-01 00:00:00
abstract:AIMS:Antibiotics and several other chemicals have been used to prevent fish diseases. However, this situation results in economic loss for the companies in the aquaculture industry and most importantly it pollutes the environment. Cinnamic acid is a naturally occurring aromatic acid and is considered to be safe for hum...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14097
更新日期:2018-09-04 00:00:00
abstract:AIMS:This meta-analysis aims to assess the effect of lactic acid bacteria (LAB) inoculation on fermentation parameters, microbiological composition and aerobic stability of corn silage. METHODS AND RESULTS:Databases (PubMed, ScienceDirect and Scopus) were searched from 1980 to 2017. The criteria for inclusion were: ra...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14084
更新日期:2018-08-24 00:00:00
abstract:AIMS:To identify and characterize adhesion-associated proteins in the potential probiotic Lactobacillus fermentum BCS87. METHODS AND RESULTS:Protein suspensions obtained from the treatment of Lact. fermentum BCS87 with 1 mol 1(-1) LiCl were analysed by Western blotting using HRP-labelled porcine mucus and mucin. Two a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04368.x
更新日期:2009-12-01 00:00:00
abstract::The genus Lactococcus comprises 12 species, some known for decades and others more recently described. Lactococcus piscium, isolated in 1990 from rainbow trout, is a psychrotrophic lactic acid bacterium, probably disregarded because most of the strains are unable to grow at 30°C. During the last 10 years, this species...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13179
更新日期:2016-10-01 00:00:00
abstract::Application of high hydrostatic pressure (200, 300, 350 and 400 MPa) at 5 degrees C for 30 min to different micro-organisms, including Gram-positive and Gram-negative bacteria, moulds and yeasts, proved to be more effective in inactivating these organisms than treatments at 20 degrees C for 10 min and at 10 degrees C ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00701.x
更新日期:1999-03-01 00:00:00
abstract:AIMS:To better understand antibiotic resistance of Enterobacter cloacae isolates originated from food animals, the phenotypic and genotypic resistance of Ent. cloacae isolates from retail ground beef, cattle farm, processing facilities and clinical settings were investigated. METHODS AND RESULTS:The ampC, ampD and amp...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03255.x
更新日期:2007-08-01 00:00:00
abstract::The distribution of micro-organisms in mature Serra, a traditional Portuguese cheese made from unpasteurised ewes' milk without added starter culture, was examined by light microscopy and electron microscopy. Four populations of micro-organisms were recognized according to their position within the cheese: (i) those p...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00375.x
更新日期:1998-04-01 00:00:00
abstract:AIMS:The effects of a new glutaraldehyde-didecyldimethylammonium bromide combination disinfectant (GD) on the cell surface of Staphylococcus aureus, a representative Gram-positive bacterium, were investigated in this study. METHODS AND RESULTS:Results of bacterial surface structural analysis showed that GD significant...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13702
更新日期:2018-05-01 00:00:00
abstract:AIM:To develop a reliable and simple method to produce safe fertilizers from human excreta using urea for sanitation of faeces. METHODS AND RESULTS:Urea was added to faecal matter (17% dry matter) at concentrations of 0.5-2% (w/w) and inactivation of Salmonella enterica subspecies 1 serovar Typhimurium (Salm. Typhimur...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04339.x
更新日期:2009-11-01 00:00:00
abstract:AIM:To elucidate the effect of chrysin on α-haemolysin production by Staphylococcus aureus and protection against pneumonia in a murine model. METHODS AND RESULTS:Haemolysis, Western blot and real-time RT-PCR assays were performed to evaluate the effect of chrysin on α-haemolysin secretion by Staph. aureus. The effica...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.05170.x
更新日期:2011-12-01 00:00:00
abstract:AIMS:To study the modification of the cell wall of Lactobacillus casei ATCC 393 grown in high salt conditions. METHODS AND RESULTS:Differences in the overall structure of cell wall between growth in high salt (MRS + 1 mol l(-1) NaCl; N condition) and control (MRS; C condition) conditions were determined by transmissio...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02428.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:This study used culture-dependent and culture-independent approaches to characterize bacterial communities in copper plumbing corrosion and to assess biofilm formation and copper resistance of heterotrophic bacteria isolated from copper pipes. METHODS AND RESULTS:Water and copper pipes were collected from a cold-...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04704.x
更新日期:2010-09-01 00:00:00
abstract::The effect of divalent cations on the inactivation of Escherichia coli by high hydrostatic pressure was investigated. The presence of 0.5 mmol l-1 of CaCl2, MgCl2, MnCl2 and FeCl2 reduced pressure inactivation of E. coli MG1655, while 0.5 mmol l-1 of ZnCl2, NiCl2, CuCl2 and CoCl2 increased inactivation. Baroprotection...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.1998.00577.x
更新日期:1998-10-01 00:00:00
abstract:AIMS:To estimate the effect of pH on the structures of bacterial community during fermentation of vegetable wastes and to investigate the relationship between bacterial community dynamics and product distribution. METHODS AND RESULTS:The bacterial communities in five batch tests controlled at different pH values [unco...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03321.x
更新日期:2007-10-01 00:00:00
abstract:AIMS:To generate continuous minimum inhibitory concentration (MIC) data that describes the discrete nature of experimentally derived population MIC data. METHODS AND RESULTS:A logistic model was fitted to experimentally derived MIC population cumulative distributions from clinical isolates of Haemophilus influenzae, M...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02842.x
更新日期:2006-05-01 00:00:00
abstract:AIMS:To investigate the effects of the endophyte Bacillus subtilisALB629 on the growth of cacao seedlings at early developmental stage and to evaluate its antimicrobial properties. METHODS AND RESULTS:Germinating cacao seeds were inoculated with ALB629, and seedlings growth was evaluated 30 days later. Significant inc...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12485
更新日期:2014-06-01 00:00:00
abstract:AIMS:Quantitative polymerase chain reaction (QPCR) methods for beach monitoring by estimating abundance of Enterococcus spp. in recreational waters use internal, positive controls which address only the amplification of target DNA. In this study two internal, positive controls were developed to control for both amplifi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03936.x
更新日期:2008-12-01 00:00:00
abstract::Techniques for the separation/concentration of micro-organisms from background food matrices can be applied to increase the speed of analysis and ease of isolation and detection of target micro-organisms. One recent example of such a technique is the immunomagnetic separation (IMS) procedure that has been used for the...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00257.x
更新日期:1997-08-01 00:00:00
abstract:AIMS:The medicinal fungi Inonotus xeranticus and Phellinus linteus in the family Hymenochaetaceae have been used as traditional medicines for the treatment of various diseases. However, the compound responsible for the antioxidant activity is still unknown. Therefore, this study was conducted to characterize the antiox...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03737.x
更新日期:2008-06-01 00:00:00
abstract:AIMS:As part of a field-based study of the distribution and persistence of Salmonella infection on commercial egg-laying farms, sampling was carried out on one or more occasions in egg-packing areas of 12 farms infected with Salm. Enteritidis. METHODS AND RESULTS:Salmonellas were isolated by cultural methods. Contamin...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01817.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To evaluate the behaviour of Shiga toxin-producing Escherichia coli (STEC) O26 strains inoculated in manure-amended soils under in vitro conditions. METHODS AND RESULTS:Four green fluorescent protein (GFP)-labelled STEC O26 strains were inoculated in duplicate (at 10(6) CFU g(-1)) in three different manure-amende...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03532.x
更新日期:2008-01-01 00:00:00
abstract:AIMS:The aim of this study was to evaluate the inactivation efficiency of Listeria monocytogenes ATC(L3) C 7644 and Salmonella enterica serovar Typhimurium strain DS88 by combined treatment of hypericin (Hyp)-based photosensitization and high power pulsed light (HPPL). METHODS AND RESULTS:Cells were incubated with Hyp...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05296.x
更新日期:2012-06-01 00:00:00
abstract:AIMS:In China, apple production areas are largely from the coastal to inland areas and across varied climate zones. However, the relationship among soil micro-organisms, environmental factors and fruit quality has not been clearly confirmed in orchards. Here we attempted to identify the variation of soil bacteria in th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14878
更新日期:2020-10-04 00:00:00
abstract::Thirty isolates of Listeria monocytogenes and 18 of L. innocua obtained from different short-ripened cheeses manufactured in Asturias (northern Spain), were compared with each other and with reference strains using serotype, phage type and pulsed-field restriction endonuclease digestion profiles analysis of the total ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00339.x
更新日期:1998-02-01 00:00:00
abstract:AIM:To introduce the gfp gene encoding green fluorescent protein (GFP) into bradyrhizobia for their identification in nodules, soil and carrier-based inoculants. METHODS AND RESULTS:Bradyrhizobium sp. strains M29 and GN7, which nodulate mungbean (Vigna radiata), were conjugated with Escherichia coli S17-1 carrying pla...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01768.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To determine the composition and temporal stability of the gut (faecal) microbiota of sheep (Ovis aries). METHODS AND RESULTS:Microbial population dynamics was conducted using ARISA (28 sheep) and 16S rRNA sequencing (11 sheep). Firmicutes and Bacteroidetes were the predominant bacterial phyla, constituting ~80% ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14468
更新日期:2020-01-01 00:00:00
abstract:AIMS:To identify dominant bacteria in grain (barley)-fed cattle for isolation and future use to increase the efficiency of starch utilization in these cattle. METHODS AND RESULTS:Total DNA was extracted from samples of the rumen contents from eight steers fed a barley diet for 9 and 14 days. Bacterial profiles were ob...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03492.x
更新日期:2007-12-01 00:00:00
abstract:AIMS:To determine the capacity of extracts of Pelargonium reniforme and Pelargonium sidoides, plants of the Geraniaceae family, to stimulate the uptake and killing of mycobacteria by murine macrophages and to identify the constituents that are responsible. METHODS AND RESULTS:Bioassay-guided fractionation of aqueous P...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04085.x
更新日期:2009-04-01 00:00:00
abstract:AIMS:The purpose of this study was to isolate, identify and characterize an antifungal compound from Lactobacillus plantarum KCC-10 from forage silage with potential beneficial properties. METHODS AND RESULTS:The 16S rRNA gene-based phylogenetic affiliation was determined using bioinformatic tools and identified as La...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12319
更新日期:2013-11-01 00:00:00