Combining spore germination and heat inactivation to decontaminate materials contaminated with Bacillus anthracis spores.

Abstract:

AIMS:To add a spore germination step in order to reduce decontamination temperature and time requirements compared to the current hot, humid air decontamination parameters, which are 75-80°C, ≥72 h, 70-90% RH, down to ≤60°C and ≤24 h total decontamination time. METHODS AND RESULTS:Bacillus anthracis spore germination with l-alanine+inosine+calcium dipicolinate (CaDPA) was quantified at 0-40°C, several time points and spore concentrations of 5-9 log10 per ml. Germination efficiency at 0-40°C was >99% at <8 log10 spores per ml. The temperature optimum was 20°C. Germination efficiency was significantly higher but slower at 0°C compared to ≥30°C at ≥8 log10 spores per ml. A single germinant application followed by 60°C, 1-h treatment consistently inactivated >2 log10 (>99%) of spores. However, a repeat application of germinant was needed to achieve the objective of ≥6 log10 spore inactivation out of a 7 log10 challenge (≥99·9999%) for ≤24 h total decontamination time for nylon and aircraft performance coating. CONCLUSIONS:l-alanine+inosine+CaDPA stimulated germination across wide temperature and spore concentration ranges. SIGNIFICANCE AND IMPACT OF THE STUDY:Germination expands the scope of spore decontamination to include materials from any industry sector that can be sprayed with an aqueous germinant solution.

journal_name

J Appl Microbiol

authors

Buhr TL,Minter ZA,Kennihan NL,Young AA,Borgers-Klonkowski EL,Osborn EB,Bohmke MD,Hamilton SM,Kimani MB,Miller CT,Mackie RS,Innocenti JM,Bensman MD,Lilly SD

doi

10.1111/jam.14474

subject

Has Abstract

pub_date

2020-01-01 00:00:00

pages

124-137

issue

1

eissn

1364-5072

issn

1365-2672

journal_volume

128

pub_type

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